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Team:Nevada/Team
From 2009.igem.org
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== '''What we did''' == | == '''What we did''' == | ||
| - | [[Nick Tschernia]] | + | <div style="padding:20px;"> |
| - | + | [[Nick Tschernia]] | |
| - | + | * Entered the Nevada Team into the iGEM Competition a week before the submission date. | |
| - | + | * Organized weekly meetings for project brainstorming and timeline. | |
| - | + | * Recruited the iGEM Team through a highly selective process (whoever was left at the end of the brainstorming sessions). | |
| - | + | * Organized the source plate distribution and transformation of the basic parts. | |
| - | + | * Sequence analysis of the Biobrick parts formed. | |
| + | * Primer design for adding Biobrick restriction sites for formation of Biobrick parts. | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | [[Leigh Armijo]] | + | [[Janice Cho]] |
| - | + | * Formulated the original idea of using cinnamaldehyde as a mosquito larvicide. | |
| - | + | * Performed initial research on the use of cinnamaldehyde. | |
| + | * Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000. | ||
| + | * Helped conduct a three-way ligation to create final construct with the parts: BBa_K262000 and BBa_K262002. | ||
| + | |||
| + | |||
| + | [[Leigh Armijo]] | ||
| + | * Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000. | ||
| + | * Helped conduct a three-way ligation to create a construct with BBa_K262000, 4-coumarate:CoA ligase, and BBa_B0014. | ||
| + | |||
[[Sheena Jones]] | [[Sheena Jones]] | ||
| - | + | * In charge of maintaining and propagating ''Wolffia'' and ''Lemna'' plant species. | |
| - | + | * Generated callus culture in preparation for gene transformation. | |
| - | + | ||
| - | + | ||
[[Tony Moschetti]] | [[Tony Moschetti]] | ||
| - | + | * Topo-cloned cinnamoyl-CoA reductase (gene 3)for preparation of a Biobrick part. | |
| - | + | * Made Biobrick part containing cinnamoyl-CoA reductase (gene 3) and double terminator (BBa_K262002) | |
| - | + | * Transformed 4-coumarate-CoA ligase (gene 2) and cinnamoyl-CoA reductase (gene 3 - BBa_K262001) into Biobrick backbones. | |
| - | + | ||
| - | + | ||
| - | + | ||
[[J Malikowski]] | [[J Malikowski]] | ||
| - | + | * Induced expression in non-biobrick expression vector containing 4-coumarate-CoA ligase (gene 2). Detection of product formation at 311 nm. Further studies will be conducted in the near future. | |
| - | + | * Helped to troubleshoot minipreps and other protocols. Resident expert on minipreps. | |
| - | + | * Trained Tyler to be an excellent Molecular Biologist. | |
| - | + | * Advisory role at lab meetings (How does the Harper lab do it?) | |
| - | + | ||
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| - | + | ||
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| - | [[ | + | [[Tyler Peterson]] |
| - | + | * Major player in media preparation, agarose gels, stock solutions | |
| - | + | * Helped with minipreps and ''E. coli'' transformation. | |
| - | + | ||
| + | |||
| + | [[Chris Martinez]] | ||
| + | * Made db3.1 and NEB10 competent cells. | ||
| + | * Overall goal was to upregulate the cinnamaldehyde pathway in plants ''Wolffia'' and various ''Lemna'' species | ||
| + | * Transformation of cinnamoyl-CoA Reductase (Gene 3) into TOPO D Vector | ||
| + | * Used LR CLonase Protocal to move cinnamoyl-CoA Reductase (Gene 3) into pK7WG2D.1 Binary Vector which will be used to grow up Agrobacterium, which will be used to infect the plants ''Wolffia'' and ''Lemna'' species | ||
| + | |||
| + | |||
| + | [[Joey Khwaja]] | ||
| + | * Made db3.1 and NEB10 competent cells. | ||
| + | * Researched into making a Biobrick plant part. | ||
| + | * Designed the iGEM Nevada Team shirts. | ||
| + | </div> | ||
== '''Meet the Team''' == | == '''Meet the Team''' == | ||
Revision as of 11:13, 20 October 2009
Back: Dr. Shintani, J, Alex, Tyler. Middle: Janice, Leigh, Joey, Chris. Front: Nick and Dr. Howard
Contents |
Who we are
Advisors
- Advisor 1: Dr. Christie Howard
- Advisor 2: Dr. Patricia Ellison
- Advisor 3: Dr. Dave Shintani
- Grad Student 1: Chad Sanada
Undergrads
- Student 1: Nick Tschernia
- Student 2: Tony Moschetti
- Student 3: Joey Khwaja
- Student 4: Chris Martinez
- Student 5: Janice Cho
- Student 6: Leigh Armijo
- Student 7: Sheena Jones
- Student 8: Tyler Peterson
- Student 9: J Malikowski
- Student 10: Alex Dussaq
What we did
- Entered the Nevada Team into the iGEM Competition a week before the submission date.
- Organized weekly meetings for project brainstorming and timeline.
- Recruited the iGEM Team through a highly selective process (whoever was left at the end of the brainstorming sessions).
- Organized the source plate distribution and transformation of the basic parts.
- Sequence analysis of the Biobrick parts formed.
- Primer design for adding Biobrick restriction sites for formation of Biobrick parts.
- Formulated the original idea of using cinnamaldehyde as a mosquito larvicide.
- Performed initial research on the use of cinnamaldehyde.
- Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000.
- Helped conduct a three-way ligation to create final construct with the parts: BBa_K262000 and BBa_K262002.
- Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000.
- Helped conduct a three-way ligation to create a construct with BBa_K262000, 4-coumarate:CoA ligase, and BBa_B0014.
- In charge of maintaining and propagating Wolffia and Lemna plant species.
- Generated callus culture in preparation for gene transformation.
- Topo-cloned cinnamoyl-CoA reductase (gene 3)for preparation of a Biobrick part.
- Made Biobrick part containing cinnamoyl-CoA reductase (gene 3) and double terminator (BBa_K262002)
- Transformed 4-coumarate-CoA ligase (gene 2) and cinnamoyl-CoA reductase (gene 3 - BBa_K262001) into Biobrick backbones.
- Induced expression in non-biobrick expression vector containing 4-coumarate-CoA ligase (gene 2). Detection of product formation at 311 nm. Further studies will be conducted in the near future.
- Helped to troubleshoot minipreps and other protocols. Resident expert on minipreps.
- Trained Tyler to be an excellent Molecular Biologist.
- Advisory role at lab meetings (How does the Harper lab do it?)
- Major player in media preparation, agarose gels, stock solutions
- Helped with minipreps and E. coli transformation.
- Made db3.1 and NEB10 competent cells.
- Overall goal was to upregulate the cinnamaldehyde pathway in plants Wolffia and various Lemna species
- Transformation of cinnamoyl-CoA Reductase (Gene 3) into TOPO D Vector
- Used LR CLonase Protocal to move cinnamoyl-CoA Reductase (Gene 3) into pK7WG2D.1 Binary Vector which will be used to grow up Agrobacterium, which will be used to infect the plants Wolffia and Lemna species
- Made db3.1 and NEB10 competent cells.
- Researched into making a Biobrick plant part.
- Designed the iGEM Nevada Team shirts.
Meet the Team
