Team:Warsaw/Calendar-Main/23 June 2009

From 2009.igem.org

(Difference between revisions)
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<h4>Kuba</h4>
<h4>Kuba</h4>
<ul>
<ul>
-
<li>pLG612-1 digested with EcoRI and HindIII</li>
+
<br>
 +
<br>
 +
<li>'''pLG612-1 digested with EcoRI and HindIII'''</li>
</ul>
</ul>
https://static.igem.org/mediawiki/2009/2/2a/PLG(E%26H).jpg
https://static.igem.org/mediawiki/2009/2/2a/PLG(E%26H).jpg
 +
<ul>
 +
        <li>Gel (from left)</li>
 +
</ul>
 +
<ol>
 +
<li>uncut plasmid (isolate no.1)</li>
 +
<li>cut plasmid (isolate no.1)</li>
 +
<li>uncut plasmid (isolate no.2)</li>
 +
<li>cut plasmid (isolate no.2)</li>
 +
<li>uncut plasmid (isolate no.3)</li>
 +
<li>cut plasmid (isolate no.3)</li>
 +
<li>GeneRuler DNA Ladder Mix #SM0333 (Fermentas)</li>
 +
</ol>
 +
 +
<ul>
 +
<br>
 +
<br>
 +
<li>'''pVDL9 digested with NotI'''</li>
 +
</ul>
 +
https://static.igem.org/mediawiki/2009/2/2a/PLG(E%26H).jpg
 +
<ul>
 +
        <li>Gel (from left)</li>
 +
</ul>
 +
<ol>
 +
<li>GeneRuler DNA Ladder Mix #SM0333 (Fermentas)</li>
 +
<li>uncut plasmid</li>
 +
<li>cut plasmid</li>
 +
</ol>

Revision as of 23:30, 27 June 2009

digestion of plasmids pLG612-1 and pVDL9 (containing components of hemolisin secretion system)

Kuba



  • pLG612-1 digested with EcoRI and HindIII

PLG(E%26H).jpg

  • Gel (from left)
  1. uncut plasmid (isolate no.1)
  2. cut plasmid (isolate no.1)
  3. uncut plasmid (isolate no.2)
  4. cut plasmid (isolate no.2)
  5. uncut plasmid (isolate no.3)
  6. cut plasmid (isolate no.3)
  7. GeneRuler DNA Ladder Mix #SM0333 (Fermentas)


  • pVDL9 digested with NotI

PLG(E%26H).jpg

  • Gel (from left)
  1. GeneRuler DNA Ladder Mix #SM0333 (Fermentas)
  2. uncut plasmid
  3. cut plasmid
Retrieved from "http://2009.igem.org/Team:Warsaw/Calendar-Main/23_June_2009"