Team:Wisconsin-Madison/Notebook Protocols

From 2009.igem.org

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* Plasmid Preparation by Alkaline Lysis
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* [[Team:Wisconsin-Madison/Plasmid Preparation by Alkaline Lysis|Plasmid Preparation by Alkaline Lysis]]
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* Plasmid Preparation by Boiling Lysis
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* [[Team:Wisconsin-Madison/Plasmid Preparation by Boiling Lysis|Plasmid Preparation by Boiling Lysis]]
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* Restriction Digestion
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* [[Team:Wisconsin-Madison/Restriction Digestion|Restriction Enzyme Mapping]]
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* Transformation of Plasmids into Competant Cells
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* [[Team:Wisconsin-Madison/Transformation of Plasmids into Competent Cells|Transformation of Plasmids into Competent Cells]]
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* Preparation of Competent Cells
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* [[Team:Wisconsin-Madison/Preparation of Competent Cells|Preparation of Competent Cells]]
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* Cloning Protocols
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* [[Team:Wisconsin-Madison/Cloning Protocols|Overview of Cloning Protocol]] ** GREAT for New Teams! **
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* Polymerase Chain Reaction (PCR)
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* [[Team:Wisconsin-Madison/Polymerase Chain Reaction (PCR)|Polymerase Chain Reaction (PCR)]]
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* Ligation
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* [[Team:Wisconsin-Madison/Ligation|Ligation]]
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* Digestion - Cloning
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* [[Team:Wisconsin-Madison/Digestion - Cloning|Digestion - Cloning]]
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* Inoculation of Plasmids in Liquid Media
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* [[Team:Wisconsin-Madison/Loading Gel Protocol|Loading Gel Protocol]]
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* Inoculation of Cyanobacteria
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* [[Team:Wisconsin-Madison/Sequencing Reaction|Sequencing Reaction]]
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* Loading Gel Protocol
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* [[Team:Wisconsin-Madison/Colony Polymerase Chain Reaction (PCR)|Colony Polymerase Chain Reaction (PCR)]]
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* Plasmid Clean-up
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* [[Team:Wisconsin-Madison/Genomic DNA Extraction|Genomic DNA Extraction]]
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* Gel Extraction from Agar
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* [[Team:Wisconsin-Madison/Safety|Safety]]
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* Sequencing Reaction
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* Colony Polymerase Chain Reaction (PCR)
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* Genomic DNA Extraction
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Latest revision as of 18:26, 20 October 2009



PROTOCOLS