Team:Wash U/Calendar-Home/26 June 2009

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(Difference between revisions)
(→Friday June 26, 2009)
(→Friday June 26, 2009)
Line 16: Line 16:
-Gel layout:
-Gel layout:
-
Lane 1 (from left): 100 bp ladder
+
Lane 1 (from left): 1 kb ladder
-
2: Sample A
+
2: Sample A: BBa_I15010, red light sensor, 2238 bp
-
3: Sample B
+
3: Sample B: BBa_R0082, OmpC promoter, 108 bp
-
4: Sample C
+
4: Sample C: BBa_B0015, terminator, 129 bp
-
5: Sample D
+
5: Sample D: BBa_J04450
-
6: Sample E
+
6: Sample E: BBa_B0034, RBS, 12 bp
-
7: Sample F
+
7: Sample F: BBa_E0240, GFP, 976 bp
-
8: Sample G
+
8: Sample G: BBa_J13002, TetR, 74 bp
-
9: 1 kb ladder
+
9: 10 kb ladder

Revision as of 15:45, 29 June 2009



Friday June 26, 2009

-Received Sigma shipment of tubes, reagents, and restriction enzymes

-Made TAE buffer for electrophoresis gels

-Ran PCR-amplified biobrick parts (from yesterday) on 0.8% agarose gel, to check size

-Visualized gel

-Gel layout:

Lane 1 (from left): 1 kb ladder

2: Sample A: BBa_I15010, red light sensor, 2238 bp

3: Sample B: BBa_R0082, OmpC promoter, 108 bp

4: Sample C: BBa_B0015, terminator, 129 bp

5: Sample D: BBa_J04450

6: Sample E: BBa_B0034, RBS, 12 bp

7: Sample F: BBa_E0240, GFP, 976 bp

8: Sample G: BBa_J13002, TetR, 74 bp

9: 10 kb ladder


Recipe for loading:

1 μL loading dye + 8 μL sample per lane

105 volts


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