(→Friday June 26, 2009) |
(→Friday June 26, 2009) |
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| Line 24: | Line 24: | ||
4: Sample C: BBa_B0015, terminator, 129 bp | 4: Sample C: BBa_B0015, terminator, 129 bp | ||
| - | 5: Sample D: BBa_J04450 | + | 5: Sample D: BBa_J04450, RFP |
6: Sample E: BBa_B0034, RBS, 12 bp | 6: Sample E: BBa_B0034, RBS, 12 bp | ||
-Received Sigma shipment of tubes, reagents, and restriction enzymes
-Made TAE buffer for electrophoresis gels
-Ran PCR-amplified biobrick parts (from yesterday) on 0.8% agarose gel, to check size
-Visualized gel
-Gel layout:
Lane 1 (from left): 1 kb ladder
2: Sample A: BBa_I15010, red light sensor, 2238 bp
3: Sample B: BBa_R0082, OmpC promoter, 108 bp
4: Sample C: BBa_B0015, terminator, 129 bp
5: Sample D: BBa_J04450, RFP
6: Sample E: BBa_B0034, RBS, 12 bp
7: Sample F: BBa_E0240, GFP, 976 bp
8: Sample G: BBa_J13002, TetR, 74 bp
9: 10 kb ladder
Recipe for loading:
1 μL loading dye + 8 μL sample per lane
105 volts
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