Team:Kyoto/CiC/Notebook/week2
From 2009.igem.org
(Difference between revisions)
(Removing all content from page) |
|||
Line 1: | Line 1: | ||
+ | ==Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide == | ||
+ | {{:Team:Kyoto/Pad_start_dates}} | ||
+ | ===Monday, September 10=== | ||
+ | ====To Do on 24 July==== | ||
+ | *PCR (HIV TAT LALAA peptide,11R LALLA peptide) | ||
+ | *Electrophoresis | ||
+ | *Restriction Enzyme Digestion | ||
+ | ====Results==== | ||
+ | =====Electrophoresis===== | ||
+ | [[Image:Kyoto_e_090724_1.png|250px]] | ||
+ | * Very clear bands were found. (See the gel image in right!) | ||
+ | * The bands are listed below. | ||
+ | {| border="1" style="text-align:center;width:50%" | ||
+ | |+Parts and Plasmid length | ||
+ | !!!3-O-2!!3-A-4 | ||
+ | |- | ||
+ | |Parts length / bp||2837||487 | ||
+ | |- | ||
+ | |Plasmid length / bp||3266||3266 | ||
+ | |} | ||
+ | |||
+ | =====Digestion===== | ||
+ | *Digested with EcoR1 / EcoR1 and Pst1 and run on a gel. | ||
+ | |||
+ | {| border="1" style="text-align:center;width:50%" | ||
+ | |+Sample list | ||
+ | !NO.!!Sample Name!!Enzyme | ||
+ | |-align="center" | ||
+ | |1||3-O-2||Eco R1, PST1 | ||
+ | |-align="center" | ||
+ | |2||3-A-4||Eco R1, PST1 | ||
+ | |-align="center" | ||
+ | |3||3-O-2||Eco R1 | ||
+ | |-align="center" | ||
+ | |4||3-A-4||Eco R1 | ||
+ | |} | ||
+ | |||
+ | |||
+ | {{:Team:Kyoto/Pad_between_dates}} | ||
+ | |||
+ | |||
+ | |||
+ | ===Tuseday, September 11=== | ||
+ | ====To Do on 24 July==== | ||
+ | *Ligation(HIV TAT LALAA peptide,11R LALLA peptide) | ||
+ | *Transformation | ||
+ | |||
+ | ====Results==== | ||
+ | =====Ligation===== | ||
+ | *Digested with EcoR1 / EcoR1 and Pst1 and run on a gel. | ||
+ | |||
+ | {| border="1" style="text-align:center;width:50%" | ||
+ | |+Sample list | ||
+ | !NO.!!Sample Name!!Enzyme | ||
+ | |-align="center" | ||
+ | |1||3-O-2||Eco R1, PST1 | ||
+ | |-align="center" | ||
+ | |2||3-A-4||Eco R1, PST1 | ||
+ | |-align="center" | ||
+ | |3||3-O-2||Eco R1 | ||
+ | |-align="center" | ||
+ | |4||3-A-4||Eco R1 | ||
+ | |} | ||
+ | |||
+ | =====Electrophoresis===== | ||
+ | [[Image:Kyoto_e_090724_1.png|250px]] | ||
+ | * Very clear bands were found. (See the gel image in right!) | ||
+ | * The bands are listed below. | ||
+ | |||
+ | {| border="1" style="text-align:center;width:50%" | ||
+ | |+Parts and Plasmid length | ||
+ | !!!3-O-2!!3-A-4 | ||
+ | |- | ||
+ | |Parts length / bp||2837||487 | ||
+ | |- | ||
+ | |Plasmid length / bp||3266||3266 | ||
+ | |} | ||
+ | {{:Team:Kyoto/Pad_endof_dates}} | ||
+ | <html></div><!-- /#rightcontents --></html> |
Revision as of 18:48, 20 October 2009
Contents |
Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide
Monday, September 10
To Do on 24 July
- PCR (HIV TAT LALAA peptide,11R LALLA peptide)
- Electrophoresis
- Restriction Enzyme Digestion
Results
Electrophoresis
- Very clear bands were found. (See the gel image in right!)
- The bands are listed below.
3-O-2 | 3-A-4 | |
---|---|---|
Parts length / bp | 2837 | 487 |
Plasmid length / bp | 3266 | 3266 |
Digestion
- Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
NO. | Sample Name | Enzyme |
---|---|---|
1 | 3-O-2 | Eco R1, PST1 |
2 | 3-A-4 | Eco R1, PST1 |
3 | 3-O-2 | Eco R1 |
4 | 3-A-4 | Eco R1 |
Tuseday, September 11
To Do on 24 July
- Ligation(HIV TAT LALAA peptide,11R LALLA peptide)
- Transformation
Results
Ligation
- Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
NO. | Sample Name | Enzyme |
---|---|---|
1 | 3-O-2 | Eco R1, PST1 |
2 | 3-A-4 | Eco R1, PST1 |
3 | 3-O-2 | Eco R1 |
4 | 3-A-4 | Eco R1 |
Electrophoresis
- Very clear bands were found. (See the gel image in right!)
- The bands are listed below.
3-O-2 | 3-A-4 | |
---|---|---|
Parts length / bp | 2837 | 487 |
Plasmid length / bp | 3266 | 3266 |