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Team:Kyoto/CiC/Notebook/week2
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==Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide == | ==Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide == | ||
{{:Team:Kyoto/Pad_start_dates}} | {{:Team:Kyoto/Pad_start_dates}} | ||
| - | ===Monday, September | + | ===Monday, September 16=== |
====To Do on 24 July==== | ====To Do on 24 July==== | ||
| - | * | + | *Miniprep (HIV TAT LALAA peptide_terminator) |
| - | + | ||
*Restriction Enzyme Digestion | *Restriction Enzyme Digestion | ||
====Results==== | ====Results==== | ||
| - | ===== | + | =====Miniprep===== |
| - | + | *HIV TAT LALAAA_terminator was minipreped. | |
| - | * | + | |
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| - | + | ||
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=====Digestion===== | =====Digestion===== | ||
| - | *Digested with | + | *Digested with Spe1 and Pst1 / Xba1 and Pst1 and run on a gel. |
{| border="1" style="text-align:center;width:50%" | {| border="1" style="text-align:center;width:50%" | ||
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!NO.!!Sample Name!!Enzyme | !NO.!!Sample Name!!Enzyme | ||
|-align="center" | |-align="center" | ||
| - | |1|| | + | |1||1-N-23||Spe1, PST1 |
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|-align="center" | |-align="center" | ||
| - | | | + | |2||HIVTAT||Xba1, PST1 |
|} | |} | ||
Revision as of 19:03, 20 October 2009
Contents |
Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide
Monday, September 16
To Do on 24 July
- Miniprep (HIV TAT LALAA peptide_terminator)
- Restriction Enzyme Digestion
Results
Miniprep
- HIV TAT LALAAA_terminator was minipreped.
Digestion
- Digested with Spe1 and Pst1 / Xba1 and Pst1 and run on a gel.
| NO. | Sample Name | Enzyme |
|---|---|---|
| 1 | 1-N-23 | Spe1, PST1 |
| 2 | HIVTAT | Xba1, PST1 |
Tuseday, September 11
To Do on 24 July
- Ligation(HIV TAT LALAA peptide,11R LALLA peptide)
- Transformation
Results
Ligation
- Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
| NO. | Sample Name | Enzyme |
|---|---|---|
| 1 | 3-O-2 | Eco R1, PST1 |
| 2 | 3-A-4 | Eco R1, PST1 |
| 3 | 3-O-2 | Eco R1 |
| 4 | 3-A-4 | Eco R1 |
Electrophoresis
- Very clear bands were found. (See the gel image in right!)
- The bands are listed below.
| 3-O-2 | 3-A-4 | |
|---|---|---|
| Parts length / bp | 2837 | 487 |
| Plasmid length / bp | 3266 | 3266 |
