Team:Kyoto/CiC/Notebook/week2

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==Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide ==
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{{:Team:Kyoto/Pad_start_dates}}
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===Monday, September 16===
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====To Do on 24 July====
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*Miniprep (HIV TAT LALAA peptide_terminator)
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*Restriction Enzyme Digestion
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====Results====
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=====Miniprep=====
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*HIV TAT LALAAA_terminator was minipreped.
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=====Digestion=====
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*Digested with Spe1 and Pst1 / Xba1 and Pst1 and run on a gel.
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{| border="1" style="text-align:center;width:50%"
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|+Sample list
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!NO.!!Sample Name!!Enzyme
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|-align="center"
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|1||1-N-23||Spe1, PST1
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|-align="center"
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|2||HIVTAT||Xba1, PST1
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|}
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{{:Team:Kyoto/Pad_between_dates}}
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===Thursday, September 17===
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====To Do on 24 July====
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*Colony PCR(T7promoter_TAT_terminater)
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*Electrophoresis
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====Results====
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=====Electrophoresis=====
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[[Image:Kyoto_e_090724_1.png|250px]]
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* Very clear bands were found. (See the gel image in right!)
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* The bands are listed below.
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{| border="1" style="text-align:center;width:50%"
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|+Parts and Plasmid length
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!!!3-O-2!!3-A-4
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|-
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|Parts length / bp||2837||487
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|-
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|Plasmid length / bp||3266||3266
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|}
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{{:Team:Kyoto/Pad_endof_dates}}
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<html></div><!-- /#rightcontents --></html>

Latest revision as of 19:07, 20 October 2009

Contents

Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide


Monday, September 16

To Do on 24 July

  • Miniprep (HIV TAT LALAA peptide_terminator)
  • Restriction Enzyme Digestion

Results

Miniprep
  • HIV TAT LALAAA_terminator was minipreped.
Digestion
  • Digested with Spe1 and Pst1 / Xba1 and Pst1 and run on a gel.
Sample list
NO.Sample NameEnzyme
11-N-23Spe1, PST1
2HIVTATXba1, PST1





Thursday, September 17

To Do on 24 July

  • Colony PCR(T7promoter_TAT_terminater)
  • Electrophoresis

Results

Electrophoresis

Kyoto e 090724 1.png

  • Very clear bands were found. (See the gel image in right!)
  • The bands are listed below.
Parts and Plasmid length
3-O-23-A-4
Parts length / bp2837487
Plasmid length / bp32663266