Team:Kyoto/CiC/Notebook/week2

From 2009.igem.org

(Difference between revisions)
(→Tuseday, September 11)
 
(2 intermediate revisions not shown)
Line 1: Line 1:
==Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide ==
==Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide ==
{{:Team:Kyoto/Pad_start_dates}}
{{:Team:Kyoto/Pad_start_dates}}
-
===Monday, September 10===
+
===Monday, September 16===
====To Do on 24 July====
====To Do on 24 July====
-
*PCR (HIV TAT LALAA peptide,11R LALLA peptide)
+
*Miniprep (HIV TAT LALAA peptide_terminator)
-
*Electrophoresis
+
*Restriction Enzyme Digestion
*Restriction Enzyme Digestion
====Results====
====Results====
-
=====Electrophoresis=====
+
=====Miniprep=====
-
[[Image:Kyoto_e_090724_1.png|250px]]
+
*HIV TAT LALAAA_terminator was minipreped.
-
* Very clear bands were found. (See the gel image in right!)
+
-
* The bands are listed below.
+
-
 
+
-
{| border="1" style="text-align:center;width:50%"
+
-
|+Parts and Plasmid length
+
-
!!!3-O-2!!3-A-4
+
-
|-
+
-
|Parts length / bp||2837||487
+
-
|-
+
-
|Plasmid length / bp||3266||3266
+
-
|}
+
-
 
+
=====Digestion=====
=====Digestion=====
-
*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
+
*Digested with Spe1 and Pst1 / Xba1 and Pst1 and run on a gel.
{| border="1" style="text-align:center;width:50%"
{| border="1" style="text-align:center;width:50%"
Line 28: Line 15:
!NO.!!Sample Name!!Enzyme
!NO.!!Sample Name!!Enzyme
|-align="center"
|-align="center"
-
|1||3-O-2||Eco R1, PST1
+
|1||1-N-23||Spe1, PST1
|-align="center"
|-align="center"
-
|2||3-A-4||Eco R1, PST1
+
|2||HIVTAT||Xba1, PST1
-
|-align="center"
+
-
|3||3-O-2||Eco R1
+
-
|-align="center"
+
-
|4||3-A-4||Eco R1
+
|}
|}
Line 42: Line 25:
-
===Tuseday, September 11===
+
===Thursday, September 17===
====To Do on 24 July====
====To Do on 24 July====
-
*Ligation(HIV TAT LALAA peptide,11R LALLA peptide)  
+
*Colony PCR(T7promoter_TAT_terminater)  
-
*Transformation
+
*Electrophoresis
-
 
+
====Results====
====Results====
-
=====Ligation=====
 
-
*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
 
-
 
-
{| border="1" style="text-align:center;width:50%"
 
-
|+Sample list
 
-
!NO.!!Sample Name!!Enzyme
 
-
|-align="center"
 
-
|1||3-O-2||Eco R1, PST1
 
-
|-align="center"
 
-
|2||3-A-4||Eco R1, PST1
 
-
|-align="center"
 
-
|3||3-O-2||Eco R1
 
-
|-align="center"
 
-
|4||3-A-4||Eco R1
 
-
|}
 
-
 
=====Electrophoresis=====
=====Electrophoresis=====
[[Image:Kyoto_e_090724_1.png|250px]]
[[Image:Kyoto_e_090724_1.png|250px]]

Latest revision as of 19:07, 20 October 2009

Contents

Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide

Monday, September 16

To Do on 24 July

  • Miniprep (HIV TAT LALAA peptide_terminator)
  • Restriction Enzyme Digestion

Results

Miniprep
  • HIV TAT LALAAA_terminator was minipreped.
Digestion
  • Digested with Spe1 and Pst1 / Xba1 and Pst1 and run on a gel.
Sample list
NO.Sample NameEnzyme
11-N-23Spe1, PST1
2HIVTATXba1, PST1



Thursday, September 17

To Do on 24 July

  • Colony PCR(T7promoter_TAT_terminater)
  • Electrophoresis

Results

Electrophoresis

Kyoto e 090724 1.png

  • Very clear bands were found. (See the gel image in right!)
  • The bands are listed below.
Parts and Plasmid length
3-O-23-A-4
Parts length / bp2837487
Plasmid length / bp32663266

Retrieved from "http://2009.igem.org/Team:Kyoto/CiC/Notebook/week2"