Team:Kyoto/CiC/Notebook/week2

From 2009.igem.org

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(→Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide)
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===Tuseday, September 11===
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===Thursday, September 17===
====To Do on 24 July====
====To Do on 24 July====
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*Ligation(HIV TAT LALAA peptide,11R LALLA peptide)  
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*Colony PCR(T7promoter_TAT_terminater)  
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*Transformation
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*Electrophoresis
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====Results====
====Results====
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=====Ligation=====
 
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*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
 
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{| border="1" style="text-align:center;width:50%"
 
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|+Sample list
 
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!NO.!!Sample Name!!Enzyme
 
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|-align="center"
 
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|1||3-O-2||Eco R1, PST1
 
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|-align="center"
 
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|2||3-A-4||Eco R1, PST1
 
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|-align="center"
 
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|3||3-O-2||Eco R1
 
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|-align="center"
 
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|4||3-A-4||Eco R1
 
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|}
 
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=====Electrophoresis=====
=====Electrophoresis=====
[[Image:Kyoto_e_090724_1.png|250px]]
[[Image:Kyoto_e_090724_1.png|250px]]

Latest revision as of 19:07, 20 October 2009

Contents

Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide

Monday, September 16

To Do on 24 July

  • Miniprep (HIV TAT LALAA peptide_terminator)
  • Restriction Enzyme Digestion

Results

Miniprep
  • HIV TAT LALAAA_terminator was minipreped.
Digestion
  • Digested with Spe1 and Pst1 / Xba1 and Pst1 and run on a gel.
Sample list
NO.Sample NameEnzyme
11-N-23Spe1, PST1
2HIVTATXba1, PST1



Thursday, September 17

To Do on 24 July

  • Colony PCR(T7promoter_TAT_terminater)
  • Electrophoresis

Results

Electrophoresis

Kyoto e 090724 1.png

  • Very clear bands were found. (See the gel image in right!)
  • The bands are listed below.
Parts and Plasmid length
3-O-23-A-4
Parts length / bp2837487
Plasmid length / bp32663266

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