"
Team:Wash U/Calendar-Home/1 September 2009
From 2009.igem.org
(Difference between revisions)
| (3 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| + | {{WashUback}} | ||
| + | |||
| + | |||
| + | == Tuesday September 1, 2009 == | ||
| + | |||
| + | |||
9/1 Minipreps | 9/1 Minipreps | ||
| Line 19: | Line 25: | ||
|} | |} | ||
| - | Did 2 digests of RBS30_cph8 with 1.5 ug each according to prefix-insertion instructions from the Silver lab- cut with EcoRI and PstI | + | Did 2 digests of RBS30_cph8 with 1.5 ug each according to prefix-insertion instructions from the Silver lab- cut with EcoRI and PstI (A1 and A2) |
| - | Did 1 digest each of 1.5 ug of ompR_term_ompC_GFP and ompR_term_ompC_pucB/A as the vector for prefix insertion- cut with EcoRI and XbaI. | + | Did 1 digest each of 1.5 ug of ompR_term_ompC_GFP (B) and ompR_term_ompC_pucB/A (C) as the vector for prefix insertion- cut with EcoRI and XbaI. |
The Results of this digest are: | The Results of this digest are: | ||
| - | + | The first 4 lanes are the two rxns for RBS30_cph8. The top band is the one that was cutout (ab. 2.3 kb) | |
| - | + | ||
| - | The first 4 lanes are the two rxns for RBS30_cph8. The top band is the one that was cutout | + | |
The next 2 lanes are ompR_term_ompC_GFP and the last two are ompR_term_ompC_pucB/A | The next 2 lanes are ompR_term_ompC_GFP and the last two are ompR_term_ompC_pucB/A | ||
| + | |||
| + | [[Image:9_1 gel purify.jpg]] | ||
| + | |||
| + | |||
| + | |||
| + | Concentrations: of products after Gel purification: | ||
| + | |||
| + | A1) A2) B) C) | ||
| + | |||
| + | |||
| + | The products were ligated in the following ratios after gel purification for 1 hr at 22 deg C, followed by 20 min at 80 deg C: | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | <div style="text-align: right;"> | ||
| + | <div style="float: left;"> | ||
| + | [[Team:Wash_U/Calendar-Home/31 August 2009|Previous entry]]</div> | ||
| + | [[Team:Wash_U/Calendar-Home/14 September 2009|Next entry]]</div> | ||
| + | <div style="clear: left;"></div> | ||
| + | |||
| + | <center>[[Team:Wash U/Notebook|Back to Calendar]]</center> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | {{WashUbottom}} | ||
Latest revision as of 20:47, 20 October 2009
