Team:Washington/Project

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(Idealized Protein Production and Purification)
 
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== Traditional Protein Purification vs Our System: Ideal Protein Purification ==
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The ability to quickly and cheaply generate purified proteins is a useful skill for aspiring synthetic biologists and iGEM participants alike, since purified proteins are used in many key laboratory processes.  Although some commonly used proteins (like the restriction enzymes EcoRI, XbaI, SpeI, and PstI) can be bought, many applications require synthetic biologists to purify their own proteins, either to study or for use in atypical biological processes.  Many members of our team have personal experience purifying proteins and have found it to be a slow and tedious process. For these reasons, the UW 2009 iGEM team has designed an Idealized Protein Purification (IPP) scheme designed to speed up protein purification, in the hopes of making protein purification facile and accessible for synthetic biologists, as well as potentially making previously difficult-to-purify proteins easier to generate.
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!align="center"|[[Team:Washington/Project/Target|Target Vector]]
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!align="center"|[[Team:Washington/Project/Secretion|Secretion System]]
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!align="center"|[[Team:Washington/Project/Display|Display System]]
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==Traditional Protein Production and Purification==
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ENTER IN PROJECT BACKGROUND INFO HERE!
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[[Image:OldPurificationGraphic.png|500px|center]]
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<!--- The Mission, Experiments --->
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Recombinant protein purification<sup>1</sup> is a method whose basic steps have not changed significantly in over two decades - thus we believe this process is due for an overhaul.  Traditional protein purification steps require expensive and specialized equipment (such as an ultracentrifuge and columns that bind His tags) that may not be accessible to the average synthetic biologist.  Traditional protein production and purification typically consists of the following three protein production steps and six purification steps:
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(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
 
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*TRADITIONAL PROTEIN PRODUCTION<sup>1</sup>
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#  Clone in a favorite protein (referenced here as afp) into an expression plasmid designed to generate lots of protein fused with a His tag.
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#  Transform this plasmid into an expression strain of bacteria specialized in protein production.
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#  Culture cells and induce protein expression, allowing cells to grow to high density.
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*TRADITIONAL PROTEIN PURIFICATION<sup>1</sup>
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#  Pellet cells, re-suspend in a lysis buffer and incubate to ensure full cell lysis, releasing proteins trapped within the cell into the media. 
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#  Subject cell debris to a long, high-speed spin in an ultra-centrifuge to remove remaining insoluble matter (membrane, insoluble proteins, etc) and leave soluble proteins in the supernatant.
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#  Filter the supernatant to remove any lingering debris before being added to an affinity column.  This ensures that the columns do not get clogged.
 +
#  Run the filtered supernatant over an affinity column several times to ensure thorough binding of His-tagged Afp to affinity column.
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#  Flow wash buffer over column to eliminate proteins that non-specifically bind to the column.
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#  Collect Afp by running an elution buffer over the column to release Afp.
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== '''Idealized Protein Purification''' ==
 
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Purified proteins are an absolute necessity for aspiring synthetic biologists and iGEM participants alike, being used in many key laboratory processes.  Though commonly used proteins like EcoRI, XbaI, SpeI, and PstI can be bought, those who research proteins and protein design, must purify their own proteinsMany of us have personal experience dully purifying protein, which makes the field as a whole slower and more tedious that it could be. For these reasons, the UW 2009 iGEM team has decided to design an Idealized Protein Purification scheme that will speed up protein purification and possibly make previously difficult to purify proteins easier to work with, in the hopes to make synthetic biology a little faster, and a little more accessible.
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Though not necessarily hard, the traditional purification process is tedious and time consuming, taking us about three and a half hours to complete on purification dayWith this in mind, we created an idealized protein purification scheme that is easier, cheaper and faster than the traditional method.
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==Idealized Protein Production and Purification==
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First, a review of the commonly used purification schemes
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[[Image:NewPurificationGraphic.png|500px|center]]
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To avoid all the laborious purification steps present in traditional protein purification we designed a system (Ideal Protein Purification) in which the cell, not the researcher, performs the purification steps to make recombinant protein product. Ideal Protein Purification reduces purification to two easy steps (cloning remains the same):
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The first step of traditional protein purification schemes is cloning in a favorite protein (referenced here as afp) into an expression plasmid designed to generate lots of protein. This plasmid is then transformed into an expression strain of bacteria streamlined for protein production, which is then cultured, induced, and allowed to grow to high density.  When they’re ready, these cells are pelletized and re-suspended in a lysis buffer, releasing all the proteins.  After 30 minutes, the lysate is spun on an ultra-centrifuge for 1 hour to pellet insoluble cell debris, leaving soluble proteins in the supernatant.  This supernatant is then filtered to remove any lingering debris before being added to an affinity column.  After running the proteins through the column a few times, a wash buffer is used to eliminate proteins that non-specifically bind to the column.  Finally an elution buffer is added, releasing your favorite protein.  Though not necessarily hard, this process is tedious and time consuming, taking us about three and a half hours to complete on purification day.  With this in mind, we created an idealized protein purification scheme that makes protein purification facile, that takes a fraction of the time older methods require.
 
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*IDEALIZED PROTEIN PRODUCTION (IPP)
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Our system is designed to secrete proteins of interest and then displays them on the cell’s surface, eliminating the lysis, filtration, and most centrifuge stepsFirst, clone a favorite protein into the target construct, an expression plasmid that adds a secretion tag and a display tag to the proteinThen transform the target plasmid into cells already containing a secretion plasmid, which contains all the parts of a type 1 secretion system, and a display plasmid, which expresses a protein that can non-covalently bond the display tag.  Culture the colonies, and induce when appropriateOnce the proteins are ready to be collected, spin down the cells, and re-suspend in an elution buffer, which contains a small molecule that disrupts the binding between display tag and display protein, causing your protein to fall off into the mediaPellet the cells again and take the supernatant which contains your purified protein. This process should take about 10 min, quick enough to do between classes. 
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# Clone afp into the IPP "target vector"This creates a fusion protein in which a "display tag" <sup>2</sup> (called the Nano-Tag) is fused to the to the N-terminus of Afp, and a "secretion tag" to the C-terminus.   
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#  Transform the target vector with the afp insert into cells already containing two additional plasmids.  The first is a "Secretion plasmid", which contains all the parts of a type 1 secretion system from ''Erwinia chrysanthemi'' <sup>3</sup> that specifically recognizes proteins that harbor the secretion tag and exports them outside of the cell.  The second is a "Display plasmid" which expresses a protein that is directed to the cell surface <sup>4</sup> and can bind to the "display tag", and then subsequently release the display tag when a specific small molecule is introduced to the system<sup>2</sup>.   
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#  Grow up cells in our specialized cell line that contains both Secretion and Display plasmids and induce the expression of afp, just as in the traditional protein purification methodAs the protein is produced, it gets secreted to the media via the secretion system, and then binds to the outside of the cells via the display system.  Now your protein of interest is non-covalently attached to the outside of the cell.
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*IDEALIZED PROTEIN PURIFICATION
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#  Spin down the cells and re-suspend in an elution buffer containing a small molecule which disrupts the interaction between the display tag and the protein it was binding to on the surface of the cellsThis causes Afp to be released from the surface of the cell.
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#  Collect Afp by pelleting the cells again and keeping the supernatant, which contains your purified protein.
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In addition to saving time, our idealized protein purification method has other potential advantages over older methods.  By keeping the cells intact, contaminants are not released into the media, which should result in purer protein product.  A host of proteins toxic to E. Coli could still be grown by secreting them before reaching high concentrations.  Alternately, some proteins can’t properly fold in E. Coli, but by secreting these proteins into the appropriate media, they might fold correctly.  Finally, there are some applications, such a detoxification, where it is desirable to secrete protein into the environment, which our system could be easily modified to do.
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This process should take about 10 minutes, quick enough to do between classes!  In addition to saving time, our idealized protein purification method has other potential advantages over older methods.  By keeping the cells intact, contaminants are not released into the media, which should result in purer protein product.  A host of proteins normally toxic to ''E. coli'' in the traditional protein purification system could be grown in the Ideal Protein Purification system since they would be secreted outside of the cell before reaching high intra-cellular concentrations.  Alternately, some proteins cannot properly fold in ''E. coli'', but by secreting these proteins into the appropriate media, they might fold correctly.  Finally, there are some environmental applications, such as detoxification, where it is desirable to have a bacterium capable of secreting protein into the environment, which our system could be easily modified to do.
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== Project Details==
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<div style="text-align:right">'''Continue to [https://2009.igem.org/Team:Washington/Project/Target Target &gt;]'''</div>
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----
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====Citations====
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=== Part 2 ===
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#Protein production and purification, Structural Genomics Consortium et.al. Nature Methods Vol.5 NO.2, February 2008
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#TAG: Lamla and Erdmann. [http://www.ncbi.nlm.nih.gov/pubmed/14680960 The Nano-tag, a streptavidin-binding peptide for the purification and detection of recombinant proteins.]
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#Palacios et al. [http://www.ncbi.nlm.nih.gov/pubmed/11157948 Subset of Hybrid Eukaryotic Proteins Is Exported by the Type I Secretion System of Erwinia chrysanthemi, Secretion of GFP in E. Coli]
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#Cell Surface Streptavidin, P. Tsai. IET Synth. Biol., Vol. 1, No. 1–2, 2007
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=== The Experiments ===
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=== Part 3 ===
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== Results ==
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Latest revision as of 20:58, 20 October 2009

Uw title logo.png

Traditional Protein Purification vs Our System: Ideal Protein Purification

The ability to quickly and cheaply generate purified proteins is a useful skill for aspiring synthetic biologists and iGEM participants alike, since purified proteins are used in many key laboratory processes. Although some commonly used proteins (like the restriction enzymes EcoRI, XbaI, SpeI, and PstI) can be bought, many applications require synthetic biologists to purify their own proteins, either to study or for use in atypical biological processes. Many members of our team have personal experience purifying proteins and have found it to be a slow and tedious process. For these reasons, the UW 2009 iGEM team has designed an Idealized Protein Purification (IPP) scheme designed to speed up protein purification, in the hopes of making protein purification facile and accessible for synthetic biologists, as well as potentially making previously difficult-to-purify proteins easier to generate.

Traditional Protein Production and Purification

OldPurificationGraphic.png

Recombinant protein purification1 is a method whose basic steps have not changed significantly in over two decades - thus we believe this process is due for an overhaul. Traditional protein purification steps require expensive and specialized equipment (such as an ultracentrifuge and columns that bind His tags) that may not be accessible to the average synthetic biologist. Traditional protein production and purification typically consists of the following three protein production steps and six purification steps:


  • TRADITIONAL PROTEIN PRODUCTION1
  1. Clone in a favorite protein (referenced here as afp) into an expression plasmid designed to generate lots of protein fused with a His tag.
  2. Transform this plasmid into an expression strain of bacteria specialized in protein production.
  3. Culture cells and induce protein expression, allowing cells to grow to high density.
  • TRADITIONAL PROTEIN PURIFICATION1
  1. Pellet cells, re-suspend in a lysis buffer and incubate to ensure full cell lysis, releasing proteins trapped within the cell into the media.
  2. Subject cell debris to a long, high-speed spin in an ultra-centrifuge to remove remaining insoluble matter (membrane, insoluble proteins, etc) and leave soluble proteins in the supernatant.
  3. Filter the supernatant to remove any lingering debris before being added to an affinity column. This ensures that the columns do not get clogged.
  4. Run the filtered supernatant over an affinity column several times to ensure thorough binding of His-tagged Afp to affinity column.
  5. Flow wash buffer over column to eliminate proteins that non-specifically bind to the column.
  6. Collect Afp by running an elution buffer over the column to release Afp.


Though not necessarily hard, the traditional purification process is tedious and time consuming, taking us about three and a half hours to complete on purification day. With this in mind, we created an idealized protein purification scheme that is easier, cheaper and faster than the traditional method.

Idealized Protein Production and Purification

NewPurificationGraphic.png

To avoid all the laborious purification steps present in traditional protein purification we designed a system (Ideal Protein Purification) in which the cell, not the researcher, performs the purification steps to make recombinant protein product. Ideal Protein Purification reduces purification to two easy steps (cloning remains the same):


  • IDEALIZED PROTEIN PRODUCTION (IPP)
  1. Clone afp into the IPP "target vector". This creates a fusion protein in which a "display tag" 2 (called the Nano-Tag) is fused to the to the N-terminus of Afp, and a "secretion tag" to the C-terminus.
  2. Transform the target vector with the afp insert into cells already containing two additional plasmids. The first is a "Secretion plasmid", which contains all the parts of a type 1 secretion system from Erwinia chrysanthemi 3 that specifically recognizes proteins that harbor the secretion tag and exports them outside of the cell. The second is a "Display plasmid" which expresses a protein that is directed to the cell surface 4 and can bind to the "display tag", and then subsequently release the display tag when a specific small molecule is introduced to the system2.
  3. Grow up cells in our specialized cell line that contains both Secretion and Display plasmids and induce the expression of afp, just as in the traditional protein purification method. As the protein is produced, it gets secreted to the media via the secretion system, and then binds to the outside of the cells via the display system. Now your protein of interest is non-covalently attached to the outside of the cell.
  • IDEALIZED PROTEIN PURIFICATION
  1. Spin down the cells and re-suspend in an elution buffer containing a small molecule which disrupts the interaction between the display tag and the protein it was binding to on the surface of the cells. This causes Afp to be released from the surface of the cell.
  2. Collect Afp by pelleting the cells again and keeping the supernatant, which contains your purified protein.


This process should take about 10 minutes, quick enough to do between classes! In addition to saving time, our idealized protein purification method has other potential advantages over older methods. By keeping the cells intact, contaminants are not released into the media, which should result in purer protein product. A host of proteins normally toxic to E. coli in the traditional protein purification system could be grown in the Ideal Protein Purification system since they would be secreted outside of the cell before reaching high intra-cellular concentrations. Alternately, some proteins cannot properly fold in E. coli, but by secreting these proteins into the appropriate media, they might fold correctly. Finally, there are some environmental applications, such as detoxification, where it is desirable to have a bacterium capable of secreting protein into the environment, which our system could be easily modified to do.

Continue to Target >

Citations

  1. Protein production and purification, Structural Genomics Consortium et.al. Nature Methods Vol.5 NO.2, February 2008
  2. TAG: Lamla and Erdmann. [http://www.ncbi.nlm.nih.gov/pubmed/14680960 The Nano-tag, a streptavidin-binding peptide for the purification and detection of recombinant proteins.]
  3. Palacios et al. [http://www.ncbi.nlm.nih.gov/pubmed/11157948 Subset of Hybrid Eukaryotic Proteins Is Exported by the Type I Secretion System of Erwinia chrysanthemi, Secretion of GFP in E. Coli]
  4. Cell Surface Streptavidin, P. Tsai. IET Synth. Biol., Vol. 1, No. 1–2, 2007