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| - | <title>Theoretical background</title>
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| - | </head>
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| - | <body>
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| - | <!-- First table displays centered iGEM 2009 Logo -->
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| - | <div id="header">
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| - | <img src="wiki banner_967px.png" />
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| - | </div>
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| - | <!-- Table displaying top row of links -->
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| - | <table class="links" >
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| - | <tr>
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| - | <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark" >Home</a></font> </td>
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| - | <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/team" >The Team</a> </font></td>
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| - | <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/project" >The Project</a> </font></td>
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| - | <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/parts" >Parts submitted</a> </font></td>
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| - | <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/modelling">Modelling</a></font> </td>
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| - | <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/notebook" title="Day to day lab activity">Notebook</a>
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| - | <td width="163px" height="100%" valign="top">
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| - | <font color="#990000" face="arial" size="3">
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| - | <br>
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| - | The redoxilator<br><br>
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| - | </font>
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| - | <font color="" face="arial" size="2">
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| - | <a href="theory" CLASS=leftbar>- Theoretical background</a><br>
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| - | <a href="yeast" CLASS=leftbar>- Yeast as a model organism</a><br>
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| - | <a href="practicalapproach" CLASS=leftbar>- Practical approach</a><br>
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| - | </font>
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| - | <font color="#990000" face="arial" size="3">
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| - | <br>The USER assembly standard<br><br>
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| - | </font>
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| - | <font face="arial" size="2">
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| - | <a href="http://www.yahoo.com" CLASS=leftbar>- Principle</a><br>
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| - | <a href="http://www.yahoo.com" CLASS=leftbar>- Proof of concept</a><br>
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| - | <a href="http://www.yahoo.com" CLASS=leftbar>- Manual</a><br>
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| - | <a href="http://www.yahoo.com" CLASS=leftbar>- Primer design software</a><br>
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| - | </font>
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| - | </td>
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| - | <b>The project</b><br><br><br>
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| - | <!-- INSERT MAIN TEXT HERE! (formatting: <b>bold</> <i>italic> <h4>header</h4>) -->
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| - | <font size="4"><b>Project abstract</b></font><br><br>
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| - | <font size="3"><b>The Redoxilator</b></font><br>
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| - | <p align="justify">By in silico design and computer modelling followed by gene synthesis, we have constructed a molecular NAD/NADH ratio sensing system in Saccharomyces cerevisiae. The sensor works as an inducible transcription factor being active only at certain levels of the NAD/NADH ratios. By the coupling of a yeast optimized fast degradable GFP, the system can be used for in vivo monitoring of NAD/NADH redox poise. A future novel application of the system is heterologous redox coupled protein production in yeast.</p>
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| - | [[Image:regulation.redox.jpg|300px|thumb|center|The redox coupled system]]
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| - | <font size="3"><b>The USER fusion standard</b></font><br>
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| - | <p align="justify">Another part of our project is the proposal of a new parts-assembly standard for Biobricks based on USER(TradeMark) cloning. With this technique, not based on restriction enzymes, all parts independent of function can be assembled without leaving any scars from the restriction enzyme digestions.</p>
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| - | <td width="182px" height="100%" valign="top" bgcolor=DDDDDD class="greybox">
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| - | <b>The yeast metabolic cycle</b><br><br>
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| - | <p align="justify">It has recently been shown by Tu <i>et al.</i> and Klevecz <i>et al.</i> that the expression of at least half of the genes monitored on a standard yeast gene chip will oscillate in a coordinated manner when grown under glucose limited conditions. The cells will shift between oxidative and reductive metabolism in a synchronized metabolic cycle with three phases: oxidative, reductive/building and reductive/
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| - | charging. As oxygen will only be consumed in the oxidative phase, the dissolved oxygen will oscillate. Many metabolites and cofactors including NADH and NAD+ will also oscillate during this cycle as NADH is converted to NAD+ when oxygen is consumed.</p>
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| - | Comments or questions to the team? Please <a href="mailto:igem@bio.dtu.dk" CLASS=email>Email us</a> -- Comments of questions to webmaster? Please <a href="mailto:lronn@bio.dtu.dk" CLASS=email>Email us</a>
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