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| - | <title>The project</title>
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| - | </style>
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| - | </head>
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| - | <body>
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| - | <!-- First table displays centered iGEM 2009 Logo -->
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| - | <div id="header">
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| - | <img src="wiki banner_967px.png" />
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| - | </div>
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| - | <!-- Table displaying top row of links -->
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| - | <table class="links" >
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| - | <tr>
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| - | <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark" >Home</a></font> </td>
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| - | <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/team" >The Team</a> </font></td>
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| - | <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/project" >The Project</a> </font></td>
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| - | <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/parts" >Parts submitted</a> </font></td>
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| - | <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/modelling">Modelling</a></font> </td>
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| - | <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2009.igem.org/Team:DTU_Denmark/notebook" title="Day to day lab activity">Notebook</a>
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| - | </font></td>
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| - | <td width="163px" height="100%" valign="top">
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| - | <font color="#990000" face="arial" size="3">
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| - | <br>
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| - | The redoxilator<br><br>
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| - | </font>
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| - | <font color="" face="arial" size="2">
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| - | <a href="theory" CLASS=leftbar>- Theoretical background</a><br>
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| - | <a href="yeast" CLASS=leftbar>- Yeast as a model organism</a><br>
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| - | <a href="practicalapproach" CLASS=leftbar>- Practical approach</a><br>
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| - | <br>
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| - | </font>
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| - | <font color="#990000" face="arial" size="3">
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| - | <br>The USER assembly standard<br><br>
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| - | </font>
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| - | <font face="arial" size="2">
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| - | <a href="https://2009.igem.org/Team:DTU_Denmark/USERprinciple" CLASS=leftbar>- Principle</a><br>
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| - | <a href="https://2009.igem.org/Team:DTU_Denmark/USERconcept" CLASS=leftbar>- Proof of concept</a><br>
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| - | <a href="https://2009.igem.org/Team:DTU_Denmark/USERmanual" CLASS=leftbar>- Manual</a><br>
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| - | <a href="https://2009.igem.org/Team:DTU_Denmark/USERprogram" CLASS=leftbar>- Primer design software</a><br>
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| - | </font>
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| - | </td>
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| - | <td width="556px" height="100%" valign="top">
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| - | <font color="#990000" face="arial" size="5">
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| - | <br>
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| - | <b>The project</b><br><br><br>
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| - | </font>
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| - | <font color="#333333" face="arial" size="2.5">
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| - | <!-- INSERT MAIN TEXT HERE! (formatting: <b>bold</> <i>italic> <h4>header</h4>) -->
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| - | <font size="4"><b>Practical approach</b></font><br><br>
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| - | <font size="3"><b>In short here are the milestones of the project:</b></font><br><br>
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| - | <b>1.</b> Perform in silico modelling of NAD<sup>+</sup>/NADH oscillations, Rexivator activity at different NAD<sup>+</sup>/NADH ratios,
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| - | reporter gene degradation time, productivity, and more. This should among other things lead to the construction of computer-based model of the sensor system based on NAD<sup>+</sup>/NADH binding affinity to REX and NAD<sup>+</sup>/NADH level in yeast.<br><br>
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| - | <b>2.</b> Construct a functional NAD<sup>+</sup>/NADH sensing system in yeast with a reporter-gene output. All DNA sequences
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| - | of interest will be chemically synthesized.<br><br>
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| - | <b>3.</b> Run glucose limited fermentation and evaluate oscillative behavior of the reporter expression (quantitatively)
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| - | in relation to the yeast metabolic cycle.<br><br>
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| - | <b>4.</b> Using NAD<sup>+</sup>/NADH sensing system to construct a production-strain that produces only in a specific
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| - | phase of the metabolic cycle (e.g. when NAD<sup>+</sup> levels are high).<br><br>
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| - | <b>5.</b> Run glucose limited fermentation, and evaluate oscillative behavior of the productivity (quantitatively)
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| - | in relation to the yeast metabolic cycle. Compare productivity over time between strains.<br><br>
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| - | <b>6.</b> If necessary, change circuit logic for the production to be in a different phase of the yeast metabolic
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| - | cycle.<br><br>
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| - | <b>7.</b> Optimize the system by remodeling, based on the previous results.
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| - | </font>
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| - | <td width="182px" height="100%" valign="top" bgcolor=DDDDDD class="greybox">
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| - | <b>Synthetic Biology</b><br><br>
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| - | <!-- INSERT GREY BOX TEXT HERE! (formatting: <b>bold</> <i>italic> <h4>header</h4>) -->
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| - | <p align="left"><i>“Synthetic Biology is an art of engineering new biological systems that don’t exist in nature.”</i><br></p>
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| - | <p align="right"><i>-Paras Chopra & Akhil Kamma</i><br><br></p>
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| - | <p>In nature, biological molecules work together in complex systems to serve purposes of the cell. In synthetic biology these molecules are used as individual functional units that are combined to form tailored systems exhibiting complex dynamical behaviour. From ‘design specifications’ generated from computational modelling, engineering-based approaches enables the construction of such new specified gene-regulatory networks. The ultimate goal of synthetic biology is to construct systems that gain new functions, and the perspectives of the technology are enormous. It has already been used in several medical projects2 and is predicted to play a major role in biotech-production and environmental aspects.</p>
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| - | Comments or questions to the team? Please <a href="mailto:igem@bio.dtu.dk" CLASS=email>Email us</a> -- Comments of questions to webmaster? Please <a href="mailto:lronn@bio.dtu.dk" CLASS=email>Email us</a>
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