"
Team:Wash U/Calendar-Home/26 June 2009
From 2009.igem.org
(Difference between revisions)
(→Friday June 26, 2009) |
|||
| (14 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | + | {{WashUback}} | |
| - | -Made | + | |
| + | |||
| + | |||
| + | == Friday June 26, 2009 == | ||
| + | |||
| + | -Received Sigma shipment of tubes, reagents, and restriction enzymes | ||
| + | |||
| + | -Made TAE buffer for electrophoresis gels | ||
| + | |||
| + | -Ran PCR-amplified biobrick parts (from yesterday) on 0.8% agarose gel, to check size | ||
| + | |||
| + | -Visualized gel | ||
| + | |||
| + | -Gel layout: | ||
| + | |||
| + | Lane 1 (from left): 1 kb ladder | ||
| + | |||
| + | 2: Sample A: BBa_I15010, red light sensor, 2238 bp | ||
| + | |||
| + | 3: Sample B: BBa_R0082, OmpC promoter, 108 bp | ||
| + | |||
| + | 4: Sample C: BBa_B0015, terminator, 129 bp | ||
| + | |||
| + | 5: Sample D: BBa_J04450, RFP | ||
| + | |||
| + | 6: Sample E: BBa_B0034, RBS, 12 bp | ||
| + | |||
| + | 7: Sample F: BBa_E0240, GFP, 976 bp | ||
| + | |||
| + | 8: Sample G: BBa_J13002, TetR, 74 bp | ||
| + | |||
| + | 9: 10 kb ladder | ||
| + | |||
| + | |||
| + | Recipe for loading: | ||
| + | |||
| + | 1 μL loading dye + 8 μL sample per lane | ||
| + | |||
| + | 105 volts | ||
| + | |||
| + | |||
| + | <div style="text-align: right;"> | ||
| + | <div style="float: left;"> | ||
| + | [[Team:Wash_U/Calendar-Home/25 June 2009|Previous entry]]</div> | ||
| + | [[Team:Wash_U/Calendar-Home/29 June 2009|Next entry]]</div> | ||
| + | <div style="clear: left;"></div> | ||
| + | |||
| + | <center>[[Team:Wash U/Notebook|Back to Calendar]]</center> | ||
| + | |||
| + | |||
| + | |||
| + | {{WashUbottom}} | ||
Latest revision as of 19:57, 29 June 2009
