Team:Wash U/Calendar-Home/26 June 2009

(Difference between revisions)

Latest revision as of 19:57, 29 June 2009

-Received Sigma shipment of tubes, reagents, and restriction enzymes

-Made TAE buffer for electrophoresis gels

-Ran PCR-amplified biobrick parts (from yesterday) on 0.8% agarose gel, to check size

-Visualized gel

-Gel layout:

Lane 1 (from left): 1 kb ladder

2: Sample A: BBa_I15010, red light sensor, 2238 bp

3: Sample B: BBa_R0082, OmpC promoter, 108 bp

4: Sample C: BBa_B0015, terminator, 129 bp

5: Sample D: BBa_J04450, RFP

6: Sample E: BBa_B0034, RBS, 12 bp

7: Sample F: BBa_E0240, GFP, 976 bp

8: Sample G: BBa_J13002, TetR, 74 bp

9: 10 kb ladder

Recipe for loading:

1 μL loading dye + 8 μL sample per lane

105 volts

@@ Line 1: / Line 1: @@
--Received Sigma shipment of tubes, reagents (but no enzymes or miniprep kits)
+{{WashUback}}
+== Friday June 26, 2009 ==
+-Received Sigma shipment of tubes, reagents, and restriction enzymes
 -Made TAE buffer for electrophoresis gels
+-Ran PCR-amplified biobrick parts (from yesterday) on 0.8% agarose gel, to check size
+-Visualized gel
+-Gel layout:
+Lane 1 (from left): 1 kb ladder
+: Sample A: BBa_I15010, red light sensor, 2238 bp
+: Sample B: BBa_R0082, OmpC promoter, 108 bp
+: Sample C: BBa_B0015, terminator, 129 bp
+: Sample D: BBa_J04450, RFP
+: Sample E: BBa_B0034, RBS, 12 bp
+: Sample F: BBa_E0240, GFP, 976 bp
+: Sample G: BBa_J13002, TetR, 74 bp
+: 10 kb ladder
+Recipe for loading:
+&mu;L loading dye + 8 &mu;L sample per lane
+volts
+ <div style="text-align: right;">
+<div style="float: left;">
+[[Team:Wash_U/Calendar-Home/25 June 2009|Previous entry]]</div>
+ [[Team:Wash_U/Calendar-Home/29 June 2009|Next entry]]</div>
+<div style="clear: left;"></div>
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+{{WashUbottom}}