Team:Wash U/Calendar-Home/29 June 2009

(Difference between revisions)

Revision as of 19:59, 29 June 2009

-Made LB plates

-Carried out digestion of plasmids

-Carried out gel electrophoresis of digested plasmids

-Carried out transformations of Biobrick parts

@@ Line 4: / Line 4: @@
 == Monday June 29, 2009 ==
-Recipe for 0.8% agarose gel:
-) Add 0.8 g agarose to 50 mL 1X TAE buffer (1 ml 50X stock solution + 49 mL water)
-) Microwave on high for ~30-40 seconds (until agarose dissolved).  Stop at 10-second intervals to swirl flask.
-) Cool, then add 2.5 microliters EtBr (wear gloves!).  Swirl, and pour into gel tray.  Let sit until ready for electrophoresis.
 -Made LB plates