Team:Freiburg bioware/Testpage

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  <li><a href="https://2009.igem.org/Team:Freiburg_bioware"
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class="active"><span class="l"></span><span
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class="r"></span><span class="t">Home</span></a></li>
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  <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Team"><span
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class="l"></span><span class="r"></span><span
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class="t">The Team</span></a>
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      <li><a href="#">Overview</a></li>
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      <li><a href="#">Photos</a></li>
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class="r"></span><span class="t">The
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class="r"></span><span class="t">Human
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Practice</span></a>
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href="https://2009.igem.org/Team:Freiburg_bioware/Notebook"><span
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class="art-PostHeader">Welcome</span> </h2>
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<h1>Team Freiburg Bioware</h1>
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<br />
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<p>The first German team ever to participate in iGEM is back
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again and
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after last year's second place we're highly motivated &nbsp;to make
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some good piece of synthetic biology in 2009. In this year we want to
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create an universal restriction enzyme to facilitate labwork
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and enable new techniques. <br />
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<br />
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We're looking forward to meeting you on this year's jamboree!<br />
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<h2 class="art-PostHeaderIcon-wrapper"> <span
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class="art-PostHeader">Project Summary</span> </h2>
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Universal Endonuclease &ndash; Cutting Edge Technology</b>
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<p>Gene technology is driven by the use of restriction
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endonucleases.
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Yet, constraints of limited sequence length and variation recognized by
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available restriction enzymes pose a major roadblock for synthetic
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biology. We developed the basis for universal restriction enzymes,
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primarily for routine cloning but also with potential for in vivo
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applications. We use a nucleotide cleavage domain fused to a binding
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domain, which recognizes a programmable adapter that mediates binding
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to DNA and thus cleavage. As adapter we use readily available modified
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oligonucleotides, as binding domain anticalins and as cleavage domain
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FokI moieties engineered for heterodimerization and activity. For
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cloning, this universal enzyme has merely to be mixed with the sequence
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specific oligonucleotide and the target DNA. Binding and release are
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addressed with thermocycling. Additionally, we provide concepts for in
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vivo applications by external adapter delivery, activity regulation by
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photo-switching, as well as for modifying an argonaute protein towards
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a DNA endonuclease.
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<div class="t">FREiGEM 2009</div>
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<p><a href="https://2009.igem.org/Team:Freiburg_bioware/Team"><span
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style="font-weight: bold;"><img
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style="border: 0px solid ; width: 138px; height: 40px;"
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alt="Team"
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src="https://static.igem.org/mediawiki/2009/f/f6/Freiburg09_Team.gif" /></span></a><br />
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<b>The Team</b><br />
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In 2009 our team consists of &nbsp;14 undergraduates and 4 advisors.<br />
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<a href="https://2009.igem.org/Team:Freiburg_bioware/Team">Read
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more...</a></p>
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<p><span style="font-weight: bold;"><a
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href="http://www.bioss.uni-freiburg.de"><img
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style="border: 0px solid ; width: 144px; height: 48px;"
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alt="bioss"
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src="https://static.igem.org/mediawiki/2009/3/34/Freiburg09_Bioss_portlet.jpg" /></a><br />
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Bioss</span><br />
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We want to thank our main sponsor Bioss for supporting our project.<br />
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<a href="http://www.bioss.uni-freiburg.de">Read more...</a></p>
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Latest revision as of 22:03, 20 October 2009