From 2009.igem.org
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| - | {{:Team:BCCS-Bristol/Header}}
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| - | {{:Team:BCCS-Bristol/NotebookHeader}}
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| - | ===Week 1===
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| - | ====Canditate Proteins for Biobricks====
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| - | *Isolated 3 canditate proteins to act as carriers for our biobricks. These are FhuA,Fiu & OsmE. Started to design primers to amplify the selected genes via PCR.
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| - | *Primers designed and ordered. Waiting for their arrival to do PCR! :D
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| - | ====Reporters,RBS,Backbones====
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| - | *Decided to use 3 reporter genes, 1 RBS, 1 High Copy plasmid backbone for now.
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| - | =====Reporters=====
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| - | *RFP(Bba_E1010)
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| - | *GFP(Bba_E1040)
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| - | *LacZ(Bba_I732005)
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| - | =====RBS=====
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| - | *Bba_J61100 - From Anderson Family
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| - | =====Plasmid Backbone=====
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| - | *BBa_J04450 ; pSB1A3
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| - | *Tried to extract DNA from the iGEM biobricks and transfrom into bacteria.
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| - | *Transformations do not work properly with non-commercial E.coli strain (XL-1).
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| - | *Transformations worked the 2nd time round with commercial Nova Blue E.coli Strain. DNA samples in toolkit must be of low concentrations!
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| - | *Regrew bacterial colonies to amplify DNA of reporters,RBS,backbone.
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| - | *Miniprepped the DNA of Reporters,RBS,plasmid backbone and made glycerol stocks.
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| - | *Realised that we are faced with a problem when wanting to assemble biobricks for protein fusions.
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Latest revision as of 23:47, 20 October 2009
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