Team:Wash U/Calendar-Home/29 June 2009

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(New page: Recipe for 0.8% agarose gel: 1) Add 0.8 g agarose to 50 mL 1X TAE buffer (1 ml 50X stock solution + 49 mL water) 2) Microwave on high for ~30-40 seconds (until agarose dissolved). Stop ...)
 
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Recipe for 0.8% agarose gel:
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1) Add 0.8 g agarose to 50 mL 1X TAE buffer (1 ml 50X stock solution + 49 mL water)
 
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2) Microwave on high for ~30-40 seconds (until agarose dissolved).  Stop at 10-second intervals to swirl flask.
 
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3) Cool, then add 2.5 microliters EtBr (wear gloves!).  Swirl, and pour into gel tray.  Let sit until ready for electrophoresis.
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== Monday June 29, 2009 ==
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-Made LB plates; restreaked cultures
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-Carried out digestion of plasmids
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-Carried out gel electrophoresis of digested plasmids
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-Carried out [http://partsregistry.org/Help:Transformation_Protocol transformations] of Biobrick parts
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Latest revision as of 15:30, 30 June 2009


Monday June 29, 2009

-Made LB plates; restreaked cultures

-Carried out digestion of plasmids

-Carried out gel electrophoresis of digested plasmids

-Carried out [http://partsregistry.org/Help:Transformation_Protocol transformations] of Biobrick parts


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