Team:Wash U/Calendar-Home/29 June 2009

(Difference between revisions)

Latest revision as of 15:30, 30 June 2009

-Made LB plates; restreaked cultures

-Carried out digestion of plasmids

-Carried out gel electrophoresis of digested plasmids

-Carried out [http://partsregistry.org/Help:Transformation_Protocol transformations] of Biobrick parts

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 {{WashUback}}
 == Monday June 29, 2009 ==
+-Made LB plates; restreaked cultures
+-Carried out digestion of plasmids
-Recipe for 0.8% agarose gel:
+-Carried out gel electrophoresis of digested plasmids
-) Add 0.8 g agarose to 50 mL 1X TAE buffer (1 ml 50X stock solution + 49 mL water)
+-Carried out [http://partsregistry.org/Help:Transformation_Protocol transformations] of Biobrick parts
-) Microwave on high for ~30-40 seconds (until agarose dissolved).  Stop at 10-second intervals to swirl flask.
-) Cool, then add 2.5 microliters EtBr (wear gloves!).  Swirl, and pour into gel tray.  Let sit until ready for electrophoresis.
+ <div style="text-align: right;">
+<div style="float: left;">
+[[Team:Wash_U/Calendar-Home/26 June 2009|Previous entry]]</div>
+ [[Team:Wash_U/Calendar-Home/30 June 2009|Next entry]]</div>
+<div style="clear: left;"></div>
--Made LB plates
+<center>[[Team:Wash U/Notebook|Back to Calendar]]</center>
--Carried out digestion of plasmids
--Carried out gel electrophoresis of digested plasmids
--Carried out transformations of Biobrick parts
+{{WashUbottom}}