Team:Stanford/NotebookPage/22 July 2009

From 2009.igem.org

(Difference between revisions)
(→Daily Work)
Line 9: Line 9:
* Begin wiki revamp
* Begin wiki revamp
* Learn lab techniques
* Learn lab techniques
 +
*Familiarity w/ protocols for mini-preps and transformations
 +
*Successful mini-prep concentrations
 +
'''Project'''
'''Project'''
Line 15: Line 18:
'''Accomplishments'''
'''Accomplishments'''
*Documented Wiki work
*Documented Wiki work
 +
*Two rounds of mini-preps - both had low results
 +
[[Image:MiniPrep2.png |thumb|left|Mini-Prep 2 w/ Epoch Kit ]]
 +
*One Round of transformations
 +
[[Image:T22.png |thumb|left|Transformation Data]]
 +
 +
Line 21: Line 30:
'''Problems'''
'''Problems'''
-
*
+
*Low concentrations/ poor results
 +
 
'''Alternative Solutions'''
'''Alternative Solutions'''
-
*
+
*Get more familiar w/ lab techniques
 +
*Switch away from kit
 +
 
 +
'''Protocols'''
 +
*[[Media: Miniprep.doc | Plasmid DNA Miniprep Kit Protocol]]
 +
*[[Media: Transformation.pdf | Transformation protocol
==Events==
==Events==

Revision as of 06:23, 21 October 2009

Home Project Modeling Parts Notebook Team SBOL-V

June
MTWTFSS
[http://2009.igem.org/Team:Stanford/NotebookPage/1_June_2009 1] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/2_June_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/3_June_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/4_June_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/5_June_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/6_June_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/7_June_2009&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/8_June_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/9_June_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/10_June_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/11_June_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/12_June_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/13_June_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/14_June_2009&action=edit 14]
[http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/15_June_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/16_June_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/17_June_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/18_June_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/19_June_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/20_June_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/21_June_2009&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/22_June_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/23_June_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/24_June_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/25_June_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/26_June_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/27_June_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/28_June_2009&action=edit 28]
[http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/29_June_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/30_June_2009&action=edit 30]
July
MTWTFSS
    [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/1_July_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/2_July_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/3_July_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/5_July_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/6_July_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/7_July_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/8_July_2009&action=edit 8] [http://2009.igem.org/Team:Stanford/NotebookPage/9_July_2009 9] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/10_July_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/12_July_2009&action=edit 12]
[http://2009.igem.org/Team:Stanford/NotebookPage/13_July_2009 13] [http://2009.igem.org/Team:Stanford/NotebookPage/14_July_2009 14] [http://2009.igem.org/Team:Stanford/NotebookPage/15_July_2009 15] [http://2009.igem.org/Team:Stanford/NotebookPage/16_July_2009 16] [http://2009.igem.org/Team:Stanford/NotebookPage/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/19_July_2009&action=edit 19]
[http://2009.igem.org/Team:Stanford/NotebookPage/20_July_2009 20] [http://2009.igem.org/Team:Stanford/NotebookPage/21_July_2009 21] [http://2009.igem.org/Team:Stanford/NotebookPage/22_July_2009 22] [http://2009.igem.org/Team:Stanford/NotebookPage/23_July_2009 23] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/24_July_2009&action=edit 24] [http://2009.igem.org/Team:Stanford/NotebookPage/25_July_2009 25] [http://2009.igem.org/Team:Stanford/NotebookPage/26_July_2009 26]
[http://2009.igem.org/Team:Stanford/NotebookPage/27_July_2009 27] [http://2009.igem.org/Team:Stanford/NotebookPage/28_July_2009 28] [http://2009.igem.org/Team:Stanford/NotebookPage/29_July_2009 29] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/30_July_2009&action=edit 30] [http://2009.igem.org/Team:Stanford/NotebookPage/31_July_2009 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/2_August_2009&action=edit 2]
[http://2009.igem.org/Team:Stanford/NotebookPage/3_August_2009 3] [http://2009.igem.org/Team:Stanford/NotebookPage/4_August_2009 4] [http://2009.igem.org/Team:Stanford/NotebookPage/5_August_2009 5] [http://2009.igem.org/Team:Stanford/NotebookPage/6_August_2009 6] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/7_August_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/9_August_2009&action=edit 9]
[http://2009.igem.org/Team:Stanford/NotebookPage/10_August_2009 10] [http://2009.igem.org/Team:Stanford/NotebookPage/11_August_2009 11] [http://2009.igem.org/Team:Stanford/NotebookPage/12_August_2009 12] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/13_August_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/14_August_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/15_August_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/16_August_2009&action=edit 16]
[http://2009.igem.org/Team:Stanford/NotebookPage/17_August_2009 17] [http://2009.igem.org/Team:Stanford/NotebookPage/18_August_2009 18] [http://2009.igem.org/Team:Stanford/NotebookPage/19_August_2009 19] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/20_August_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/21_August_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/22_August_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/23_August_2009&action=edit 23]
[http://2009.igem.org/Team:Stanford/NotebookPage/24_August_2009 24] [http://2009.igem.org/Team:Stanford/NotebookPage/25_August_2009 25] [http://2009.igem.org/Team:Stanford/NotebookPage/26_August_2009 26] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/27_August_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/28_August_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/29_August_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/30_August_2009&action=edit 30]
[http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/31_August_2009&action=edit 31]
September
MTWTFSS
  [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/1_September_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/2_September_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/3_September_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/4_September_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/5_September_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/6_September_2009&action=edit 6]
[http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/7_September_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/8_September_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/9_September_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/10_September_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/11_September_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/12_September_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/13_September_2009&action=edit 13]
[http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/14_September_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/15_September_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/16_September_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/17_September_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/18_September_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/19_September_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/20_September_2009&action=edit 20]
[http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/21_September_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/22_September_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/23_September_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/24_September_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/25_September_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/26_September_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/27_September_2009&action=edit 27]
[http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/28_September_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/29_September_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/30_September_2009&action=edit 30]
October
MTWTFSS
      [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/1_October_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/2_October_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/3_October_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/4_October_2009&action=edit 4]
[http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/5_October_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/6_October_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/7_October_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/8_October_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/9_October_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/10_October_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/11_October_2009&action=edit 11]
[http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/12_October_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/13_October_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/14_October_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/15_October_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/16_October_2009&action=edit 16] [http://2009.igem.org/Team:Stanford/NotebookPage/17_October_2009 17] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/18_October_2009&action=edit 18]
[http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/19_October_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/20_October_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/21_October_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/22_October_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/23_October_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/24_October_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/25_October_2009&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/26_October_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/27_October_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/28_October_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/29_October_2009&action=edit 29] [http://2009.igem.org/Team:Stanford/NotebookPage/30_October_2009 30] [http://2009.igem.org/wiki/index.php?title=Team:Stanford/NotebookPage/31_October_2009&action=edit 31]


Contents

Daily Work

Goals

  • Write-up and establish Wiki goals
  • Begin wiki revamp
  • Learn lab techniques
  • Familiarity w/ protocols for mini-preps and transformations
  • Successful mini-prep concentrations


Project

  • Mini-preps and transformations in the lab

Accomplishments

  • Documented Wiki work
  • Two rounds of mini-preps - both had low results
File:MiniPrep2.png
Mini-Prep 2 w/ Epoch Kit
  • One Round of transformations
File:T22.png
Transformation Data



  • Made more vBOL images


Problems

  • Low concentrations/ poor results


Alternative Solutions

  • Get more familiar w/ lab techniques
  • Switch away from kit

Protocols

Events

  • 10 am wiki meeting with Bobby, Ming, and Joe
    • See Accomplishments for Designs
    • Goals established and designs made
  • 11:30am Mini-preps with Jerome
    • Low Yields... will redo in the afternoon
  • 2pm Talk Bernard Palsson Clark Center S360
    • Systems Biology
  • 3:15pm Modeling Team Presentation
  • Lab Work w/ Isis
    • Transformations
    • Mini-preps redo

Comments

Weekly Goals

  • Work through the code for templates
Retrieved from "http://2009.igem.org/Team:Stanford/NotebookPage/22_July_2009"