Team:Utah State/Notebook

From 2009.igem.org

(Difference between revisions)
Line 104: Line 104:
       </tr>
       </tr>
     <tr>
     <tr>
-
       <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Contact"><font size = 4>CONTACT </font></a></td>
+
       <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Contact"><font size = 4>CONTACT</font></a></td>
       </tr>
       </tr>
     </table></td>
     </table></td>
 +
<!---TEXT BOXES--->
     <td><table width=100% style="background:#CCCCCC; padding:7px; border-style:none">
     <td><table width=100% style="background:#CCCCCC; padding:7px; border-style:none">
-
         
+
  </td></tr>
 +
    <tr>
 +
    <td>
 +
      <font size="5" face="Century Gothic, Arial, San Serif" color =#000033>
 +
              <b><i>Notebook
 +
</b></i></font>
 +
              <HR>
 +
 
 +
  <p class = "class">
 +
This page is for the notebook - this includes notes from our official team meetings, weekly progress updates, and the lab protocols.
 +
  </P> </td></tr>
 +
</table>
 +
 
 +
<!---Official Meeting Notes--->
 +
 
 +
<br>
 +
<table width=100% style="background:#CCCCCC; padding:7px; border-style:none">
 +
  <tr>
 +
    <td>
 +
</td></tr>
 +
<tr>
 +
    <td>
 +
      <a name="Meeting">
 +
    </td>
 +
    </tr>
 +
    <tr>
 +
    <td>
 +
    <b><i><font size="4" face="Helvetica, Arial, San Serif" color =#000033>
 +
Notes from all of our Meetings
 +
    </font></b></i> <hr>
 +
<b><i>May 12<b><i>
 +
Introduction to iGEM and to team members. Reviewed last years competition. Overview of the iGEM website.
 +
Plan-Become familiar with website, review team projects from previous years, create user accounts.
 +
June 23
 +
Discussed University of Hawaii team project from 2008. Watched their presentation and discussed their project goals. Decided as a group to continue their project where they left off, after having made contact with them.
 +
Plan-Request constructs and parts from team Hawaii. Have conference call with last year's Hawaii team. Literature search.
 +
June 30
 +
Team introduction to working with a wiki. Waiting for constructs.
 +
Plan-Contribute name, brief autobiographical description, and picture to wiki.
 +
August ?
 +
Project Progress Check.
 +
Broad-Host Vector: After PCR, ligation attempts, gel observations, it appears that the Broad-Host Vector in the catalog is not functioning nor is it the anticipated length. Intend to take a known broad-host vector, perform an enzyme digestion and test ability of vector to carry red flourescent protein sequence.
 +
September 3
 +
September 10
 +
Finalized team roster and travel information. Reviewed judging criteria, our current standing as far as BioBricks designed, what could still be designed and feasibly tested. Discussed the track in which we would compete. Assignments made for next meeting.
 +
Plan-Complete abstract, finalize title and track by Sept. 18.
 +
1. Cole Peterson - Set up ligation of pRL1383a/BBa_I20260 and test.
 +
2. Alex Hatch - Submit schedule for availability to Cole, assist Cole with vector construction, write team background and post on the wiki.
 +
3. Garrett Hinton - Write a general project description for the front page of the wiki (see last years wiki, and other team's wiki's to get an idea of what you should write about and ask Libby, Trent, or Cole if you've any questions.
 +
4. Tyrell Rupp- Have a draft-version of a flash animation for our project.
 +
5. Rachel Porter- Have 3 logo ideas ready for the team to choose from.
 +
6. Jody Jerez/Jeff Karren- Edit/Post protocols on Wiki (you will need to contact other people in lab for help with revisions). Edit these so that they are also not just cut and paste from last years.
 +
September 17
 +
Had final discussions on project title and track we intend to compete in. Reviewed the project abstract. Discussed Wiki progress.
 +
</p>
 +
  </td>
 +
  </tr></tr>
 +
  </table>
 +
<br>
 +
 
 +
<!---Broad-Host Range Vectors--->
 +
<table width=100% style="background:#CCCCCC; padding:7px; border-style:none">
 +
  <tr>
 +
    <td>
 +
</td></tr>
 +
<tr>
 +
    <td>
 +
      <a name="broadhostvectors">
 +
    </td>
 +
    </tr>
 +
    <tr>
 +
    <td>
 +
    <b><i><font size="3" face="Helvetica, Arial, San Serif" color =#000033>
 +
Broad-Host Range Vectors
 +
    </font></b></i> <hr>
 +
   
 +
<p class="class">
 +
Vector text
 +
</p>
 +
 
 +
    </font></p>
 +
  </td>
 +
  </tr></tr>
 +
 
 +
<!---Go To Top of Page--->
 +
 
 +
  <tr>
 +
    <td>
 +
      <font size="3" face="Century Gothic, Arial, San Serif" color =#000033 <div align="center">
 +
  <b><a href="#top">Go to Top of Page</a></b>
 +
</div> </font>
     </td>
     </td>
     </tr>
     </tr>
 +
  </table>
-
   </tr>
+
<!---Secretion Mechanisms--->
-
</table>
+
<br>
-
</html>
+
<table width=100% style="background:#CCCCCC; padding:7px; border-style:none">
 +
  <tr>
 +
    <td>
 +
</td></tr>
 +
<tr>
 +
    <td>
 +
      <a name="secretion">
 +
    </td>
 +
    </tr>
 +
    <tr>
 +
    <td>
 +
    <b><i><font size="3" face="Helvetica, Arial, San Serif" color =#000033>
 +
Secretion: GFP, Phasins, and Bioplastics
 +
    </font></b></i> <hr>
 +
<p class="class">
 +
This is where all of the text about secretion goes - First talk about secretion in general and why we care (downstream processing, etc.); next talk about what exactly we are looking at as an example (phasin/bioplastic secretion, along with GFP as an easier-to-monitor example);
 +
</p>
 +
 +
<b><i><font size="2.5" face="Helvetica, Arial, San Serif" color =#000033>
 +
Polyhydroxyalkanoates
 +
</font><br></b></i>
 +
<p class="class">
 +
text
 +
</p>
 +
 
 +
<b><i><font size="2.5" face="Helvetica, Arial, San Serif" color =#000033>
 +
Phasin
 +
</font><br></b></i>
 +
<p class="class">
 +
text
 +
</p>
 +
 
 +
<b><i><font size="2.5" face="Helvetica, Arial, San Serif" color =#000033>
 +
Green Fluorescent Protein
 +
</font><br></b></i>
 +
<p class="class">
 +
text
 +
</p>
 +
 
 +
<b><i><font size="2.5" face="Helvetica, Arial, San Serif" color =#000033>
 +
Secretion Mechanisms in Gram-Negative Microorganisms
 +
</font><br></b></i>
 +
<p class="class">
 +
text
 +
</p>
 +
 
 +
 
 +
 
 +
  </td>
 +
   </tr></tr>
 +
<!---Go To Top of Page--->
 +
 
 +
  <tr>
 +
    <td>
 +
      <font size="3" face="Century Gothic, Arial, San Serif" color =#000033 <div align="center">
 +
  <b><a href="#top">Go to Top of Page</a></b>
 +
</div> </font>
 +
    </td>
 +
    </tr>
 +
 
 +
  </table>
 +
 
 +
 
 +
  </tr></tr>
 +
  </table>
 +
<!---BACKGROUND FORMATTING--->
<html>
<html>
<head>
<head>
Line 161: Line 318:
</html>
</html>
-
<html>
 
-
<table width=100% style="background:#CCCCCC; padding:7px; border-style:none">
 
-
 
-
  <tr>
 
-
    <td>
 
-
  This is where more text can go
 
-
   
 
-
   
 
-
    </td>
 
-
   
 
-
  </tr>
 
-
 
-
  </tr>
 
-
</table></td>
 
-
  </tr>
 
-
</table>
 
-
</body>
 
-
</html>
 
-
 
-
<html>
 
-
<table width=100% style="background:#231f20; padding:7px; border-style:none">
 
-
  <tr>
 
-
    <td>
 
-
      <font size="2" face="Century Gothic, Arial, Sans-Serif" color =#FFFFFF>
 
-
              <b><i>MAY</b></i></font>
 
-
              <HR>
 
-
<font size="2" face="Century Gothic, Arial, San Serif" color =#FFFFFF>start transcribing from lab notebooks</font>
 
-
 
-
    </td>
 
-
    </tr>
 
-
  </tr>
 
-
</table>
 
-
 
-
<!--- Official Meetings --->
 
-
<table width=100% style="background:#231f20; padding:7px; border-style:none">
 
-
  <tr>
 
-
    <td>
 
-
      <font size="2" face="Century Gothic, Arial, San Serif" color =#FFFFFF>
 
-
              <b><i>JUNE</b></i></font>
 
-
              <HR>
 
-
    </td>
 
-
    </tr>
 
-
  </tr>
 
-
</table>
 
-
 
-
</html>
 
Line 221: Line 332:
</html>
</html>
-
 
+
<html>
-
==Team Meetings==
+
<head>
-
----
+
<style type="text/css">
-
;''May 12''
+
p.class{
-
:Introduction to iGEM and to team members.  Reviewed last years competition.  Overview of the iGEM website.
+
text-indent:1.0em;
-
*Plan-Become familiar with website, review team projects from previous years, create user accounts.
+
color: #231f20;
-
 
+
font-weight : none;
-
;''June 23''
+
font-family:Tahoma, Arial, Sans-serif;
-
:Discussed University of Hawaii team project from 2008.  Watched their presentation and discussed their project goals.  Decided as a group to continue their project where they left off, after having made contact with them.
+
font size="2.5";
-
*Plan-Request constructs and parts from team Hawaii.  Have conference call with last year's Hawaii team.  Literature search.
+
}
-
 
+
p.margin
-
;''June 30''
+
{
-
:Team introduction to working with a wiki.  Waiting for constructs. 
+
margin-top:0px;
-
*Plan-Contribute name, brief autobiographical description, and picture to wiki.
+
margin-bottom:0px;
-
 
+
margin-right:50px;
-
;''August ?''
+
margin-left:50px;
-
:Project Progress Check.
+
color: #231f20;
-
::Broad-Host Vector: After PCR, ligation attempts, gel observations, it appears that the Broad-Host Vector in the catalog is not functioning nor is it the anticipated length.  Intend to take a known broad-host vector, perform an enzyme digestion and test ability of vector to carry red flourescent protein sequence. 
+
font-weight : none;
-
 
+
font-family:Tahoma, Arial, Sans-serif;
-
;''September 3''
+
font size="2.5";
-
 
+
font-style: italic;
-
;''September 10''
+
}
-
:Finalized team roster and travel information.  Reviewed judging criteria, our current standing as far as BioBricks designed, what could still be designed and feasibly tested.  Discussed the track in which we would compete.  Assignments made for next meeting.
+
ul.circle {list-style-type:circle; color: #231f20;}
-
*Plan-Complete abstract, finalize title and track by Sept. 18.
+
</style>
-
:1. Cole Peterson - Set up ligation of pRL1383a/BBa_I20260 and test.
+
</head>
-
:2. Alex Hatch - Submit schedule for availability to Cole, assist Cole with vector construction, write team background and post on the wiki.
+
-
:3. Garrett Hinton - Write a general project description for the front page of the wiki (see last years wiki, and other team's wiki's to get an idea of what you should write about and ask Libby, Trent, or Cole if you've any questions.
+
-
:4. Tyrell Rupp- Have a draft-version of a flash animation for our project.
+
-
:5. Rachel Porter- Have 3 logo ideas ready for the team to choose from.
+
-
:6. Jody Jerez/Jeff Karren- Edit/Post protocols on Wiki (you will need to contact other people in lab for help with revisions). Edit these so that they are also not just cut and paste from last years. 
+
-
 
+
-
;''September 17''
+
-
:Had final discussions on project title and track we intend to compete in.  Reviewed the project abstract.  Discussed Wiki progress.
+

Revision as of 09:55, 21 October 2009

USU iGem Untitled Document

NOTEBOOK Meeting Notes
Lab Notebook
Protocols
Notebook

This page is for the notebook - this includes notes from our official team meetings, weekly progress updates, and the lab protocols.


Notes from all of our Meetings
May 12 Introduction to iGEM and to team members. Reviewed last years competition. Overview of the iGEM website. Plan-Become familiar with website, review team projects from previous years, create user accounts. June 23 Discussed University of Hawaii team project from 2008. Watched their presentation and discussed their project goals. Decided as a group to continue their project where they left off, after having made contact with them. Plan-Request constructs and parts from team Hawaii. Have conference call with last year's Hawaii team. Literature search. June 30 Team introduction to working with a wiki. Waiting for constructs. Plan-Contribute name, brief autobiographical description, and picture to wiki. August ? Project Progress Check. Broad-Host Vector: After PCR, ligation attempts, gel observations, it appears that the Broad-Host Vector in the catalog is not functioning nor is it the anticipated length. Intend to take a known broad-host vector, perform an enzyme digestion and test ability of vector to carry red flourescent protein sequence. September 3 September 10 Finalized team roster and travel information. Reviewed judging criteria, our current standing as far as BioBricks designed, what could still be designed and feasibly tested. Discussed the track in which we would compete. Assignments made for next meeting. Plan-Complete abstract, finalize title and track by Sept. 18. 1. Cole Peterson - Set up ligation of pRL1383a/BBa_I20260 and test. 2. Alex Hatch - Submit schedule for availability to Cole, assist Cole with vector construction, write team background and post on the wiki. 3. Garrett Hinton - Write a general project description for the front page of the wiki (see last years wiki, and other team's wiki's to get an idea of what you should write about and ask Libby, Trent, or Cole if you've any questions. 4. Tyrell Rupp- Have a draft-version of a flash animation for our project. 5. Rachel Porter- Have 3 logo ideas ready for the team to choose from. 6. Jody Jerez/Jeff Karren- Edit/Post protocols on Wiki (you will need to contact other people in lab for help with revisions). Edit these so that they are also not just cut and paste from last years. September 17 Had final discussions on project title and track we intend to compete in. Reviewed the project abstract. Discussed Wiki progress.


Broad-Host Range Vectors

Vector text

Go to Top of Page

Secretion: GFP, Phasins, and Bioplastics

This is where all of the text about secretion goes - First talk about secretion in general and why we care (downstream processing, etc.); next talk about what exactly we are looking at as an example (phasin/bioplastic secretion, along with GFP as an easier-to-monitor example);

Polyhydroxyalkanoates

text

Phasin

text

Green Fluorescent Protein

text

Secretion Mechanisms in Gram-Negative Microorganisms

text

Go to Top of Page


Retrieved from "http://2009.igem.org/Team:Utah_State/Notebook"