Team:UNIPV-Pavia/Methods Materials/Digestion
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<font face="Pristina" size="50" ><b>Protocols</b></font> | <font face="Pristina" size="50" ><b>Protocols</b></font> | ||
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- | + | =='''BioBrick digestion with restriction enzymes'''== | |
''(estimated time: 3 hours)'' | ''(estimated time: 3 hours)'' | ||
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*To open vectors: | *To open vectors: | ||
**a volume containing 1 µg of purified plasmid | **a volume containing 1 µg of purified plasmid | ||
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**25 µl final volume | **25 µl final volume | ||
**incubate at 37°C for 2 hours and 30 minutes | **incubate at 37°C for 2 hours and 30 minutes | ||
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+ | <tr bgcolor='yellow'><td>INGREDIENTS: | ||
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+ | '''Roche restriction enzymes''' <hr color='white' width='70%'> | ||
+ | '''Roche buffer H''' <hr color='white' width='70%'> | ||
+ | '''Pre-warmed at 37°C bath''' <hr color='white' width='70%'> | ||
+ | '''Cut and gel-extracted vector''' <hr color='white' width='70%'> | ||
+ | '''ddH2O'''</font> | ||
+ | </td></tr></table><br><br><br><br> | ||
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Latest revision as of 10:29, 21 October 2009
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BioBrick digestion with restriction enzymes
(estimated time: 3 hours)
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