Team:UNIPV-Pavia/Methods Materials/Digestion
From 2009.igem.org
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<font face="Pristina" size="50" ><b>Protocols</b></font> | <font face="Pristina" size="50" ><b>Protocols</b></font> | ||
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**incubate at 37°C for 2 hours and 30 minutes | **incubate at 37°C for 2 hours and 30 minutes | ||
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- | <br></td><td | + | <br></td><td align='center' valign='top' width='300px'> |
- | <table | + | |
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+ | <tr bgcolor='yellow'><td>INGREDIENTS: | ||
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- | + | '''Roche restriction enzymes''' <hr color='white' width='70%'> | |
- | + | '''Roche buffer H''' <hr color='white' width='70%'> | |
- | + | '''Pre-warmed at 37°C bath''' <hr color='white' width='70%'> | |
- | + | '''Cut and gel-extracted vector''' <hr color='white' width='70%'> | |
- | </td></tr></table | + | '''ddH2O'''</font> |
+ | </td></tr></table><br><br><br><br> | ||
</td></tr></table> | </td></tr></table> |
Latest revision as of 10:29, 21 October 2009
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BioBrick digestion with restriction enzymes
(estimated time: 3 hours)
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