Revision as of 19:38, 1 July 2009

Week from July 1st, to July 5th, 2009

Miniprep for:
- A8 (for dilution/transformation)
- A9 (for dilution/transformation)

We transformed 10 pg of A8 and A9 miniprepped DNA in TOP10, after a proper quantification with Nanodrop and dilution. We plated transformed bacteria and incubated them overnight at 37°C.

We infected 5 ml of LB + Amp with 10 ul of T9002 glycerol stock. We incubated the culture overnight at 37°C, 220 rpm.

We also picked a random colony from A10 plate and infected 1 ml of LB + Amp. We incubated this inoculum at 37°C, 220 rpm for 5 and 1/2 hours. Then we prepared a glycerol stock and re-filled the remaining 250 ul of culture with 5 ml of LB + Amp to grow an overnight culture (for sequencing).

Preparation of experiment with Tecan F200

Experiment with Tecan F200

Miniprep for A10 and T9002. We sent purified DNA to BMR Genomics for sequencing.

@@ Line 30: / Line 30: @@
 **A9 (for dilution/transformation)
-*We transformed 10 pg of A8 and A9 in TOP10, after a proper quantification with Nanodrop and dilution. We plated transformed bacteria and incubated them overnight at 37°C.
+*We transformed 10 pg of A8 and A9 miniprepped DNA in TOP10, after a proper quantification with Nanodrop and dilution. We plated transformed bacteria and incubated them overnight at 37°C.
 *We prepared 0.5 l of LB + Amp.