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Team:TzuChiU Formosa/Notebook
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'''2009.10.06''' OmpC promoter transformation. | '''2009.10.06''' OmpC promoter transformation. | ||
| + | Plasmid pSB1A3 biopart from 96-well plated was dissolved in 10 ul PCR water, 2 ul was used for plasmid transformation into DH5α competent cells. | ||
Revision as of 11:56, 21 October 2009
Notebook
Meeting
2009.03 IGEM recuiting Poster
People from different department groups together after they see the poster.
2009.03.14 Experience sharing
NYMU-Taipei 2008 IGEM team came to TCU and shared Experience with TZU-CHI university.
2009.05.07 Firt meeting
Introduction of IGEM and advisors.
2009.05.15 2nd meeting
Groups formed and started brain storming.
2009.05.28 3rd meeting
Brain stormung and two project out of 12 were voted.
2009.07~2009.09 English course
English conversation course on Wednesdays, Thursdays.
2009.08.09 Japanese friends visited
Interactive friendship with University of Tokyo, and Chiba University, Japan.
2009.08.31 4th meeting
Progress report of the two groups.
2009.09.14 5th meeting
chose 1 team out of 2 .
2009.09.19 6th meeting
Reference searching.
2009.09.23 7th meeting
Reference searching and Determined the experimental designs.
2009.09.30 8th meeting with adviser
Reference searching and experimental designs report to advisors.
2009.10.2 9th meeting
Duties assigned. Started WIKI editing
2009.10.11 10th meeting
Progress report and upload project, protocals to wiki.
2009.10.15 11th meeting
Progress report.
2009.10.17 12th meeting
Upload results, discussion, notebook to wiki.
2009.10.20 13th meeting
Progress report and modification of wiki with advisors.
Experiments
2009.10.06 OmpC promoter transformation. Plasmid pSB1A3 biopart from 96-well plated was dissolved in 10 ul PCR water, 2 ul was used for plasmid transformation into DH5α competent cells.
2009.10.09 Streak out OmpC promoter,pSB1A2 plasim extraction.
2009.10.11 OmpC stock.
2009.10.12 T-A cloning.
2009.10.13 T-Atransformation.
2009.10.14 Cloning PCR.
2009.10.16 T-A cloning.
2009.10.20 Digestion, Gel extration
2009.10.21 Gel extration
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