Team:HKU-HKBU/Motor Preliminary Trials

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Revision as of 12:00, 21 October 2009

Binding performance test using a membrane

We used Immobilon-P transfer membrane to evaluate the performance of streptavidin-biotin binding of bacteria onto a surface. Before cutting apart the membrane into small pieces, pre-activation was applied to it first (see protocol membrane biotinylation). After the pre-activation, the membrane was first sheared into strings manually by scissors, with the width and thickness being approximately 100μm. We then used Leica-crytomicrotome to cut the “threads” into even smaller fragments, with the length of which being 60μm. The dimension of the binding performance testing device is aproximately 100μm×60μm×100μm. Since the surface of membrane had already been activated, the polar-expression bacteria could bind onto such biotin-coated motors.

Step1. The fragmentation of the Immobolin-P membrane with the help of a mini-homogenizer

Step2.The fragmentation of the Immobolin-P membrane with a generated shearing force by moistening and followed by vortexing

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 {{Team:HKU-HKBU/header}}
-Before the final version of motor that was using the [[Team:HKU-HKBU/Protocols#Photolithography | photolithography photoetching method]] based on silicon, we did several trials to approach the qualified micromotor with the characteristics as:
+==Binding performance test using a membrane==
-.It should be shaped into very refined tiny structures.
-.It should be able to undergo chemical modifications to coat the biotin on the surface.
+We used Immobilon-P transfer membrane to evaluate the performance of streptavidin-biotin binding of bacteria onto a surface. Before cutting apart the membrane into small pieces, pre-activation was applied to it first  (see protocol [[Team:HKU-HKBU/Protocols#Membrane_Biotinylation | membrane biotinylation]]). After the pre-activation, the membrane was first sheared into strings manually by scissors, with the width and thickness being approximately 100μm. We then used Leica-crytomicrotome to cut the “threads” into even smaller fragments, with the length of which being 60μm. The dimension of the binding performance testing device is aproximately 100μm×60μm×100μm. Since the surface of membrane had already been activated, the polar-expression bacteria could bind onto such biotin-coated motors.
-.It should be rigid and stable in the bacterial medium.
-Here we report our trials by using Immobolin-P membrane which is easy to cut and coat with biotin.
+[[Image:HKU-HKBU_motor_results_1.png| left | thumb |200px|Step1. The fragmentation of the Immobolin-P membrane with the help of a mini-homogenizer]]
-.The fragmentation of the Immobolin-P membrane with the help of a mini-homogenizer
-The Immobolin-P membrane was first made wet and consequently homogenized with a mini-homogenizer.
-Results: The membrane could not be broken into small pieces.
-[[Image:HKU-HKBU_motor_results_1.png| right | thumb |200px|Fig 1. The fragmentation of the Immobolin-P membrane with the help of a mini-homogenizer]]
 [[Image:HKU-HKBU_motor_results_2.png‎| center | thumb |200px |Step2.The fragmentation of the Immobolin-P membrane with a generated shearing force by moistening and followed by vortexing]]
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