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Team:Cambridge/Project/CA03
From 2009.igem.org
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Our preliminary research indicated that carotenoids production in ''E.coli'' TOP10 was very weak. We decided to use another strain, ''E.coli'' MG1655, for in vivo expression of our devices. The amount of carotenoids produced became visibly higher. Generally, the colour became visible after incubation for about 12 hours. | Our preliminary research indicated that carotenoids production in ''E.coli'' TOP10 was very weak. We decided to use another strain, ''E.coli'' MG1655, for in vivo expression of our devices. The amount of carotenoids produced became visibly higher. Generally, the colour became visible after incubation for about 12 hours. | ||
| - | ''Lycopene-producing device (constitutive)'' | + | '''''Lycopene-producing device (constitutive)''''' |
| - | {| style="color:#CCC; background-color:#3D5089;" cellpadding=" | + | {| style="color:#CCC; background-color:#3D5089;" cellpadding="5" cellspacing="0" border="1" |
! Agar plate colonies | ! Agar plate colonies | ||
! Cell pellet from LB culture | ! Cell pellet from LB culture | ||
| - | |- style="color:#333; background-color:#FFFFFF;" cellpadding=" | + | |- style="color:#333; background-color:#FFFFFF;" cellpadding="5" cellspacing="0" border="1" |
|[[Image:Cam09_lyco_1.jpg]] | |[[Image:Cam09_lyco_1.jpg]] | ||
|[[Image:Cam09_lyco_2.jpg]] | |[[Image:Cam09_lyco_2.jpg]] | ||
Revision as of 12:07, 21 October 2009
Categories :
Project :
-
Overview
Sensitivity Tuner
--- Characterisation
--- Modelling
Colour Generators
--- Carotenoids (Orange/Red)
--- Melanin (Brown)
--- Violacein (Purple/Green)
The Future
Safety
Notebook :
Team Logistics :
Carotenoids
TESTING AND CHARACTERISATION
In vivo expression of our devices in E.coli MG1655
Our preliminary research indicated that carotenoids production in E.coli TOP10 was very weak. We decided to use another strain, E.coli MG1655, for in vivo expression of our devices. The amount of carotenoids produced became visibly higher. Generally, the colour became visible after incubation for about 12 hours.
Lycopene-producing device (constitutive)
| Agar plate colonies | Cell pellet from LB culture |
|---|---|
| 
|
| E.coli MG1655 transformed with K274110 and grown on agar plate overnight. | Cell pellet of E.coli MG1655 transformed with K274210 (from 20mL LB culture at 37°C for 24 hours). |
