Team:TzuChiU Formosa/Notebook

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Latest revision as of 13:43, 21 October 2009

Notebook

Team Meeting

2009.03 　IGEM recuiting Poster

Students from different departments got together after they saw the poster.

2009.03.14 　Experience sharing

NYMU-Taipei 2008 IGEM team came to TCU and shared Experience with students and faculty of Tzu-Chi University.

2009.05.07 　1st meeting

Introduction of iGEM and advisors.

2009.05.15 　2nd meeting

Groups formed and started brain storming.

2009.05.28　 3rd meeting

Brain storming and selected two projects out of 12.

2009.07~2009.09 　English courses

English conversation courses given on Wednesdays, Thursdays.

2009.08.09 　Japanese friends Visited

Exchange visit with students from University of Tokyo, and Chiba University, Japan.

2009.08.31 　4th meeting

Progress report of the two groups.

2009.09.14 　5th meeting

chose 1 team out of 2 .

2009.09.19 　6th meeting

Reference searching.

2009.09.23 　7th meeting

Reference search and design the experimental protocol.

2009.09.30 　8th meeting with advisers

Reference searching and experimental designs.

2009.10.2 　9th meeting

Duties assigned. Started wiki editing.

2009.10.11 　10th meeting

Progress report and upload project, protocals to wiki.

2009.10.15 　11th meeting

Progress report.

2009.10.17 　12th meeting

Upload results, discussion, notebook to wiki.

2009.10.20 　13th meeting

Progress report and modification of wiki with advisors. 　

Experiments

2009.10.06 　OmpC promoter transformation.

Plasmid pSB1A3 biopart from 96-well plated was dissolved in 10 ul PCR water, 2 ul was used for plasmid transformation into DH5α competent cells.

2009.10.08　Positive clone selection and plasmid isolation.

4:30 pm ~ 4:50 pm Pich single bacterial colony that had been transform with OmpC promoter containing plasmid and inoculate with 3ml LB medium containing Ampicillin and grow overnight (16-20 hours) in a 37℃ shaker incubator.

2009.10.09　Colony PCR to confirm OmpC promoter and plasmid isolation.

2:00 pm ~ 5:00 pm 10 ul overnight culture was used for colony PCR. Briefly 10 ul overnight culture was centrifuged at 13,000 rpm for 10 min, supernatant was removed, 500 ul autoclaved water was added fllowed by 100C incubation for 20 min and chilled on ice. 5 ul was used for colony PCR in a total 20 ul reaction with OmpC promoter specific primers. 5 ul of the PCR products was used for agarose gel electrophoresis to verify the positive clone.

2009.10.11　Prepare LB plates

8:30 am ~12:00 noon 500 ml LB-agar was prepared and autoclaved. When LB-agar was ready (temperature cold enough to hold, about 60°C), 500 ul ampicillin (50 mg/ml) and 500 ul kanamycin (50 mg/ml) were added and 14 LB-agar plates were prepared.

4:10 pm ~ 4:30 pm pSB1A3 preparation Three single colonies containing pSB1A3 plasmid were inoculated in 5ml LB medium containing 7μl ampicillin (50 mg/ml) and grow overnight (16-20 hours) in a 37℃ shaker incubator.

2009.10.11　Stock bacterial preparation and plating new plates for future used.

4:30 pm ~ 4:50 pm Two glycerol stock were prepared as follow : 0.5 ml overnight culture was added into 0.5 ml 20% glycerol stock in a 2 ml eppendorf tube, mix well and kept at -80C.

4:35pm~4:50pm Select two OmpC promoter transformed single colonies and inoculate 5ml LB contain 7μl Ampicillin and grow overnight (16-20 hours) in a 37℃ shaker incubator.

2009.10.12 　T-A cloning.

2009.10.13　T-Atransformation.

2009.10.14　Cloning PCR.

2009.10.16 　T-A cloning.

2009.10.20 　Digestion, Gel extration

2009.10.21 　Gel extration

.

@@ Line 11: / Line 11: @@
 ==Notebook==
-'''Meeting'''
+'''Team Meeting'''
@@ Line 17: / Line 17: @@
 '''2009.03''' 　IGEM recuiting Poster
-People from different department groups together after they see the poster.
+Students from different departments got together after they saw the poster.
@@ Line 23: / Line 23: @@
 NYMU-Taipei 2008 IGEM team came to TCU and shared
-Experience with TZU-CHI university.
+Experience with students and faculty of Tzu-Chi University.
-'''2009.05.07''' 　Firt meeting
+'''2009.05.07''' 　1st meeting
-Introduction of IGEM and advisors.
+Introduction of iGEM and advisors.
@@ Line 38: / Line 38: @@
 '''2009.05.28'''　 3rd meeting
-Brain stormung and two project out of 12 were voted.
+Brain storming and selected two projects out of 12.
-'''2009.07~2009.09''' 　English course
+'''2009.07~2009.09''' 　English courses
-English conversation course on Wednesdays, Thursdays.
+English conversation courses given on Wednesdays, Thursdays.
-'''2009.08.09''' 　Japanese friends visited
+'''2009.08.09''' 　Japanese friends Visited
-Interactive friendship with University of Tokyo, and Chiba University, Japan.
+Exchange visit with students from University of Tokyo, and Chiba University, Japan.
@@ Line 59: / Line 59: @@
 '''2009.09.14'''  　5th meeting
 chose 1 team out of  2 .
@@ Line 69: / Line 69: @@
 '''2009.09.23''' 　7th meeting
-Reference searching and Determined the experimental designs.
+Reference search and design the experimental protocol.
-'''2009.09.30'''  　8th meeting with adviser
+'''2009.09.30'''  　8th meeting with advisers
-Reference searching and experimental designs report to advisors.
+Reference searching and experimental designs.
 '''2009.10.2'''  　9th meeting
-Duties assigned. Started WIKI editing
+Duties assigned. Started wiki editing.
 '''2009.10.11'''  　10th meeting
@@ Line 111: / Line 111: @@
 '''2009.10.06''' 　OmpC promoter transformation.
+Plasmid pSB1A3 biopart from 96-well plated was dissolved in 10 ul PCR water, 2 ul was used for plasmid transformation into DH5α competent cells.
-'''2009.10.09'''　Streak out OmpC promoter,pSB1A2 plasim extraction.
+'''2009.10.08'''　Positive clone selection and plasmid isolation.
-'''2009.10.11'''　OmpC stock.
+:30 pm ~ 4:50 pm
+Pich single bacterial colony that had been transform with OmpC promoter containing plasmid and inoculate with 3ml LB medium containing Ampicillin and grow overnight (16-20 hours) in a 37℃ shaker incubator.
+'''2009.10.09'''　Colony PCR to confirm OmpC promoter and plasmid isolation.
+:00 pm ~ 5:00 pm
+ul overnight culture was used for colony PCR. Briefly 10 ul overnight culture was centrifuged at 13,000 rpm for 10 min, supernatant was removed, 500 ul autoclaved water was added fllowed by 100C incubation for 20 min and chilled on ice. 5 ul was used for colony PCR in a total 20 ul reaction with OmpC promoter specific primers. 5 ul of the PCR products was used for agarose gel electrophoresis to verify the positive clone.
+'''2009.10.11'''　Prepare LB plates
+:30 am ~12:00 noon
+ml LB-agar was prepared and autoclaved. When LB-agar  was ready (temperature cold enough to hold, about 60°C), 500 ul ampicillin (50 mg/ml) and 500 ul kanamycin (50 mg/ml) were added and 14 LB-agar plates were prepared.
+:10 pm ~ 4:30 pm	pSB1A3 preparation
+Three single colonies containing pSB1A3 plasmid were inoculated in 5ml LB medium containing 7μl ampicillin (50 mg/ml) and grow overnight (16-20 hours) in a 37℃ shaker incubator.
+'''2009.10.11'''　Stock bacterial preparation and plating new plates for future used.
+:30 pm ~ 4:50 pm
+Two glycerol stock were prepared as follow :
+.5 ml overnight culture was added into 0.5 ml 20% glycerol stock in a 2 ml eppendorf tube, mix well and kept at -80C.
+:35pm~4:50pm
+Select two OmpC promoter transformed single colonies and inoculate 5ml LB contain 7μl Ampicillin and grow overnight (16-20 hours) in a 37℃ shaker incubator.