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Team:SDU-Denmark/Parts
From 2009.igem.org
(Difference between revisions)
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[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=SDU-Denmark Our Parts on the registry] | [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=SDU-Denmark Our Parts on the registry] | ||
| - | *RIP (Maybe) - <partinfo>BBa_K237000</partinfo> - The heart of our project. This is the minimal sequence coding for RIP. We have submitted a ligation mixture instead of a miniprep, due to temporal issues. | + | *RIP (Maybe) - <partinfo>BBa_K237000</partinfo> - The heart of our project. This is the minimal sequence coding for RIP. We have submitted a ligation mixture instead of a miniprep, due to temporal issues. PCR of the ligation mixture indicates that RIP has been correctly inserted. |
| - | *sRIP (Maybe) - <partinfo>BBa_K237003</partinfo> - RIP with an OmpA leader sequence, designed to let RIP leave the cytoplasm. We have submitted a ligation mixture instead of a miniprep, due to temporal issues. | + | *sRIP (Maybe) - <partinfo>BBa_K237003</partinfo> - RIP with an OmpA leader sequence, designed to let RIP leave the cytoplasm. We have submitted a ligation mixture instead of a miniprep, due to temporal issues. PCR of the ligation mixture indicates that sRIP has been correctly inserted. |
*ompA (Theoretical brick) - The leader sequence used for sRIP. | *ompA (Theoretical brick) - The leader sequence used for sRIP. | ||
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*K.Prom - RBS - RIP - Term - <partinfo>BBa_K237002</partinfo> - Constitutive RIP generator | *K.Prom - RBS - RIP - Term - <partinfo>BBa_K237002</partinfo> - Constitutive RIP generator | ||
| + | =Contributions and improvements to existing parts= | ||
| + | Our biggest contribution has been proving that the backbone housed in well 1K was not psB1A3 as indicated by the partsregistry but instead psB1A2. [[Team:SDU-Denmark/Parts/backbone_troubles|Here is a full description]]. | ||
| + | Also, we believe we have improved the <partinfo>BBa_G00101</partinfo>VR primer for use with pSB1A2, psB1A3 and psB1AK3. here is the part: <partinfo>BBa_K237007</partinfo>. | ||
Revision as of 14:07, 21 October 2009
Our submitted parts
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=SDU-Denmark Our Parts on the registry]
- RIP (Maybe) - - The heart of our project. This is the minimal sequence coding for RIP. We have submitted a ligation mixture instead of a miniprep, due to temporal issues. PCR of the ligation mixture indicates that RIP has been correctly inserted.
- sRIP (Maybe) - - RIP with an OmpA leader sequence, designed to let RIP leave the cytoplasm. We have submitted a ligation mixture instead of a miniprep, due to temporal issues. PCR of the ligation mixture indicates that sRIP has been correctly inserted.
- ompA (Theoretical brick) - The leader sequence used for sRIP.
- RIP - Term - - RIP combined with a terminator
- K.Prom - RBS - RIP - Term - - Constitutive RIP generator
Contributions and improvements to existing parts
Our biggest contribution has been proving that the backbone housed in well 1K was not psB1A3 as indicated by the partsregistry but instead psB1A2. Here is a full description.
Also, we believe we have improved the VR primer for use with pSB1A2, psB1A3 and psB1AK3. here is the part: .
