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Duke University/29 June 2009
From 2009.igem.org
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(New page: Did restriction digests <br> Ran gel to check digests) |
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| - | + | [[Team:Duke | Return]] | |
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| + | ==June 29, 2009== | ||
| + | *E: Restriction digests, Gel to check digests | ||
| + | |||
| + | <pre> | ||
| + | Digest 1 | ||
| + | NdeI (FastDigest) 1 ul | ||
| + | SpeI (NEB) 1 ul | ||
| + | 10x FastDigest buffer 2 ul | ||
| + | plasmid 1 ul (~100 ng) | ||
| + | H2O 15 ul | ||
| + | 20 ul | ||
| + | Band Sizes/Lengths: 5 kb, 3 kb, 460 bp, (80bp) | ||
| + | |||
| + | Digest 2 (Did not do) | ||
| + | NdeI (FastDigest) 2 ul | ||
| + | 10x FastDigest buffer 2 ul | ||
| + | plasmid 1 ul (~100 ng) | ||
| + | H2O 15 ul | ||
| + | 20 ul | ||
| + | Band Sizes/Lengths: 5.4 kb, 3 kb | ||
| + | |||
| + | Digest 3 | ||
| + | BamHI (FastDigest) 1 ul | ||
| + | XhoI (FastDigest) 1 ul | ||
| + | 10x FastDigest buffer 2 ul | ||
| + | plasmid 1 ul (~100 ng) | ||
| + | H2O 15 ul | ||
| + | 20 ul | ||
| + | Band Sizes/Lengths: 6.6 kb, 1.8 kb | ||
| + | |||
| + | Digest 4 | ||
| + | BamHI (FastDigest) 2 ul | ||
| + | 10x FastDigest buffer 2 ul | ||
| + | plasmid 1 ul (~100 ng) | ||
| + | H2O 15 ul | ||
| + | 20 ul | ||
| + | Band Size/Length: 8.4 kb | ||
| + | </pre> | ||
| + | |||
| + | *R: Correct bands and sizes for plasmid 2 and digests 1, 3, and 4, correct band and size for plasmid 4 | ||
| + | *C: Do more tests on plasmid 2 such as sequencing to determine if plasmid is carryover, Try different primers | ||
Latest revision as of 17:32, 21 October 2009
June 29, 2009
- E: Restriction digests, Gel to check digests
Digest 1 NdeI (FastDigest) 1 ul SpeI (NEB) 1 ul 10x FastDigest buffer 2 ul plasmid 1 ul (~100 ng) H2O 15 ul 20 ul Band Sizes/Lengths: 5 kb, 3 kb, 460 bp, (80bp) Digest 2 (Did not do) NdeI (FastDigest) 2 ul 10x FastDigest buffer 2 ul plasmid 1 ul (~100 ng) H2O 15 ul 20 ul Band Sizes/Lengths: 5.4 kb, 3 kb Digest 3 BamHI (FastDigest) 1 ul XhoI (FastDigest) 1 ul 10x FastDigest buffer 2 ul plasmid 1 ul (~100 ng) H2O 15 ul 20 ul Band Sizes/Lengths: 6.6 kb, 1.8 kb Digest 4 BamHI (FastDigest) 2 ul 10x FastDigest buffer 2 ul plasmid 1 ul (~100 ng) H2O 15 ul 20 ul Band Size/Length: 8.4 kb
- R: Correct bands and sizes for plasmid 2 and digests 1, 3, and 4, correct band and size for plasmid 4
- C: Do more tests on plasmid 2 such as sequencing to determine if plasmid is carryover, Try different primers
