Team:UCSF/PAYLOAD

From 2009.igem.org

(Difference between revisions)
Line 44: Line 44:
<h3>Approach</h3>
<h3>Approach</h3>
<p>We designed a modular and generalizable scheme for attaching fluorescent polymer microspheres to the outside of our cells. To make the approach modular, we thought of our strategy as having three main interchangeable parts: 1) Bead attachment part, 2) Linker, 3) Cell attachment part. By ensuring that we make the parts interchangeable, we made the approach generalizable. For example, changing out the cell attachment part for a different one would allow us to attach beads to a different type of cell. Likewise, we could use different types of linkers including photocleavable or enzymatically cleavable ones. Changing out the bead attachment part could allow us to attach not only beads but other cargos to cells. </p>
<p>We designed a modular and generalizable scheme for attaching fluorescent polymer microspheres to the outside of our cells. To make the approach modular, we thought of our strategy as having three main interchangeable parts: 1) Bead attachment part, 2) Linker, 3) Cell attachment part. By ensuring that we make the parts interchangeable, we made the approach generalizable. For example, changing out the cell attachment part for a different one would allow us to attach beads to a different type of cell. Likewise, we could use different types of linkers including photocleavable or enzymatically cleavable ones. Changing out the bead attachment part could allow us to attach not only beads but other cargos to cells. </p>
-
 
+
</body>
-
 
+
</html>
-
 
+
[[Image:cellbeads_scheme.jpg|400px|thumb|center|'''Modular and generalizable scheme for attaching beads to cells''']]
 +
<html xmlns="http://www.w3.org/1999/xhtml">
 +
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8" />
 +
<title>Untitled Document</title>
 +
<style type="text/css">
 +
<!--
 +
body {
 +
margin-left: 20px;
 +
margin-right: 20px;
 +
width: 900px;
 +
}
 +
-->
 +
</style>
 +
<script src="Scripts/AC_ActiveX.js" type="text/javascript"></script>
 +
<script src="Scripts/AC_RunActiveContent.js" type="text/javascript"></script>
 +
</head>
 +
<body>
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>Some important early considerations for us were therefore: 1) Best to use a scheme that does not require much (or any) genetic manipulation, so that we can use our scheme quickly and easily with any cell or cell type, 2) Use well-established chemistries and reagents, 3) Be able to swap and interchange modular parts.</p>
<p>Some important early considerations for us were therefore: 1) Best to use a scheme that does not require much (or any) genetic manipulation, so that we can use our scheme quickly and easily with any cell or cell type, 2) Use well-established chemistries and reagents, 3) Be able to swap and interchange modular parts.</p>
Line 53: Line 70:
<h3>Results</h3>
<h3>Results</h3>
<p>We have been able to observe our cells exhibiting motility while tethered to &quot;cargos&quot; of fluorescent beads. Below are two representative movies, each taken using fluorescent beads with ConA (no linker). Frame rate is 15 seconds (real time) per frame. </p>
<p>We have been able to observe our cells exhibiting motility while tethered to &quot;cargos&quot; of fluorescent beads. Below are two representative movies, each taken using fluorescent beads with ConA (no linker). Frame rate is 15 seconds (real time) per frame. </p>
 +
</body>
 +
</html>
 +
<html><object width="425" height="344"><param name="movie" value="http://www.youtube.com/v/t3a3g538hf4&hl=en&fs=1&"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="http://www.youtube.com/v/t3a3g538hf4&hl=en&fs=1&" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="425" height="344"></embed></object></html>
 +
 +
<html><object width="425" height="344"><param name="movie" value="http://www.youtube.com/v/8RtyKD5-Y_0&hl=en&fs=1&"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="http://www.youtube.com/v/8RtyKD5-Y_0&hl=en&fs=1&" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="425" height="344"></embed></object></html>
 +
<html xmlns="http://www.w3.org/1999/xhtml">
 +
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8" />
 +
<title>Untitled Document</title>
 +
<style type="text/css">
 +
<!--
 +
body {
 +
margin-left: 20px;
 +
margin-right: 20px;
 +
width: 900px;
 +
}
 +
-->
 +
</style>
 +
<script src="Scripts/AC_ActiveX.js" type="text/javascript"></script>
 +
<script src="Scripts/AC_RunActiveContent.js" type="text/javascript"></script>
 +
</head>
 +
<body>
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>Here are some stillshots from the movies. </p>
<p>Here are some stillshots from the movies. </p>
<p>&nbsp;</p>
<p>&nbsp;</p>
 +
</body>
 +
</html>
 +
[[Image:cellbead1_enh.jpg]]
 +
[[Image:cellbead2_enh.jpg]]
 +
[[Image:cellbead3_enh.jpg]]
 +
<html xmlns="http://www.w3.org/1999/xhtml">
 +
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8" />
 +
<title>Untitled Document</title>
 +
<style type="text/css">
 +
<!--
 +
body {
 +
margin-left: 20px;
 +
margin-right: 20px;
 +
width: 900px;
 +
}
 +
-->
 +
</style>
 +
<script src="Scripts/AC_ActiveX.js" type="text/javascript"></script>
 +
<script src="Scripts/AC_RunActiveContent.js" type="text/javascript"></script>
 +
</head>
 +
<body>
 +
<h3>Summary and Outlook</h3>
<h3>Summary and Outlook</h3>
<p>We have shown that <strong>we can tether simple fluorescent bead &quot;cargos&quot; to our cells</strong>. We are using a simple, generalizable, and modular scheme to attach these cargos, and we will continue to try different attachment and linker parts to improve on our initial result. Some things we are working on include efficiency and robustness of attachment and internalization of beads by cells.</p>
<p>We have shown that <strong>we can tether simple fluorescent bead &quot;cargos&quot; to our cells</strong>. We are using a simple, generalizable, and modular scheme to attach these cargos, and we will continue to try different attachment and linker parts to improve on our initial result. Some things we are working on include efficiency and robustness of attachment and internalization of beads by cells.</p>

Revision as of 18:11, 21 October 2009

Untitled Document

Making Engineered Cells carry a PAYLOAD

Motivation: Why is this useful?

Once a cellular robot has detected and traveled to a site of disease in the human body, it should be able to perform a useful therapeutic task. One simple but very useful task that we envision our cells performing in the body is the targeted delivery of drugs or signaling molecules to diseased areas. This would be useful in the delivery of all sorts of substances to targeted areas in the body. Some examples include: 1) cases where a drug is toxic and off-target side effects are dose-limiting (e.g. chemotherapeutics used for cancer), 2) cases where precise delivery of a contrast agent to diseased areas would aid in diagnosis (e.g. sites of cancer metastasis), 3) cases where localized delivery of a specific bioactive molecule to a site would be therapeutically beneficial (e.g. delivery of specific growth factors to areas of wound healing and regeneration).

 

Cellbead.jpg

Untitled Document

 

Toward the end of engineering our cellular robots to carry useful cargos, we designed and began testing a generalizable scheme for tethering fluorescent polymer microspheres ("beads") to the outside of our cells.

Approach

We designed a modular and generalizable scheme for attaching fluorescent polymer microspheres to the outside of our cells. To make the approach modular, we thought of our strategy as having three main interchangeable parts: 1) Bead attachment part, 2) Linker, 3) Cell attachment part. By ensuring that we make the parts interchangeable, we made the approach generalizable. For example, changing out the cell attachment part for a different one would allow us to attach beads to a different type of cell. Likewise, we could use different types of linkers including photocleavable or enzymatically cleavable ones. Changing out the bead attachment part could allow us to attach not only beads but other cargos to cells.

Modular and generalizable scheme for attaching beads to cells

Untitled Document

 

Some important early considerations for us were therefore: 1) Best to use a scheme that does not require much (or any) genetic manipulation, so that we can use our scheme quickly and easily with any cell or cell type, 2) Use well-established chemistries and reagents, 3) Be able to swap and interchange modular parts.

For our pilot experiments this summer, we used the simplest possible parts that we could think of. For cellular attachment, we used the jack bean lectin protein Concanavalin A (ConA), which binds mainly to internal and nonreducing terminal alpha-mannosyl groups. We left the linker out in some cases, though in others we used polyethylene glycol (PEG) linkers. For bead attachment, we used either streptavidin-biotin chemistry, passive adsorption of protein on the surface of polystyrene beads, or carbodiimide chemistry with the crosslinker EDC.

To observe the cells, we coated glass-bottomed Lab-Tek II Chambered Coverglass slides with fibronectin, adhered cells to the slide, and then incubated with varying concentrations of modified beads followed by a wash step. We then visualized the cells using time-lapse microscopy.

Results

We have been able to observe our cells exhibiting motility while tethered to "cargos" of fluorescent beads. Below are two representative movies, each taken using fluorescent beads with ConA (no linker). Frame rate is 15 seconds (real time) per frame.

Untitled Document

 

Here are some stillshots from the movies.

 

Cellbead1 enh.jpg Cellbead2 enh.jpg Cellbead3 enh.jpg Untitled Document

Summary and Outlook

We have shown that we can tether simple fluorescent bead "cargos" to our cells. We are using a simple, generalizable, and modular scheme to attach these cargos, and we will continue to try different attachment and linker parts to improve on our initial result. Some things we are working on include efficiency and robustness of attachment and internalization of beads by cells.

We would also like to explore the ability of cells to carry larger bead cargos and different types of cargos (non-bead). In addition, we would like to study the physical limitations of these cells in carrying cargo. For example, it would be particular interesting to determine whether the cells can carry carry cargos through complex environments such as three-dimensional hydrogel environments, endothelial cell monolayers, and more.

 

NEXT - Future Applications

 

 



Home The Team The Project Parts Submitted to the Registry Our summer experience Notebook Human Practices
Retrieved from "http://2009.igem.org/Team:UCSF/PAYLOAD"