Team:UNICAMP-Brazil/Notebooks/October 15

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*<p style=”text-align:justify;”>We did miniprep of YEP+Adh1-YFP and the following devices: b) pJEN1+YFP, c) pJEN1+Lys, d) pDLD+YFP, e) pDLD+Lys.</p>
*<p style=”text-align:justify;”>We did miniprep of YEP+Adh1-YFP and the following devices: b) pJEN1+YFP, c) pJEN1+Lys, d) pDLD+YFP, e) pDLD+Lys.</p>
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*<p style=”text-align:justify;”>We did the pre inoculun to prepare competet yeast tomorrow.</p>

Revision as of 18:45, 21 October 2009

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YeastGuard

New strategy: pGEM

  • We sent 4 biobricks to iGEM today: pJEN1, pDLD, Lysozyme, and the devices Adh1+YFP and Adh1+Lysozyme. =)

Yeast experiments

  • We transformed the YEP+Adh1-lysozyme ligation reaction in competent E. coli and plated in LB+Amp media. Hope to have colonies later!

  • We did miniprep of YEP+Adh1-YFP and the following devices: b) pJEN1+YFP, c) pJEN1+Lys, d) pDLD+YFP, e) pDLD+Lys.

  • We did the pre inoculun to prepare competet yeast tomorrow.


Raíssa and Taís




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