Team:EPF-Lausanne/Team

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We are a team of undergraduates with an interdisiplinary background. Most of us are from the life sciences faculty but some are from microengineering, mechanical engineering and chemistry.
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We are a team of undergraduates with an interdisciplinary background. Most of us are from the School of Life Sciences but some are from microengineering, mechanical engineering and chemistry.
[[Image: Group.jpg|500px|thumb|center|Group : from left to right : up : Nicolas G, Christian, Gabriela, Tú, Nathalie, Carine, Nicolas D, Rafael, down : Heidi, Sebastian, Caroline, Mélanie]]
[[Image: Group.jpg|500px|thumb|center|Group : from left to right : up : Nicolas G, Christian, Gabriela, Tú, Nathalie, Carine, Nicolas D, Rafael, down : Heidi, Sebastian, Caroline, Mélanie]]
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<strong>Undergrads </strong><br /><br />
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<strong>UNDERGRADS </strong><br /><br />
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<strong>Instructors </strong><br /><br />
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<strong>INSTRUCTORS </strong><br /><br />
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Image:Nicolasepfl.jpg|Nicolas Dénervaud
Image:Nicolasepfl.jpg|Nicolas Dénervaud
Image:Carine.jpg|Carine Gubelmann
Image:Carine.jpg|Carine Gubelmann
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Image:Marco_Stenta.jpg|Marco Stenta
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Image:Enrico_Spiga.jpg|Enrico Spiga
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<strong>Advisors </strong><br /><br />
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<strong>ADVISORS </strong><br /><br />
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Image:bart.jpg|Bart Deplancke
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Image:sebastian.jpg|Sebastian Maerkl
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Image:felix.jpg|Felix Naef
 
Image:matteo.jpg|Matteo Dal Peraro
Image:matteo.jpg|Matteo Dal Peraro
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== '''What we want to do''' ==
 
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Light-sensitive proteins can easily be found in nature, but they have never been cloned into other cells. In this project, our aim is to design a fusion protein that would allow genetic regulation through light control.
 
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Therefore we are working on cloning strategies that would allow us to fuse a light-sensitive domain (LovTAP in our case) with a regulatory domain (like the Trp operon). The idea is to allow transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain. This transmitted conformational change would then result in an increase or decrease of the regulatory domain's affinity for the DNA promoter site.
 
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The overal effect would thus be a genetic expression controlled by light! There would be many applications to such a "switch" : it could kill bacteria at a certain point, stop their growth, or make them express specific proteins...
 
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To improve the change induced by light (which is generally very unstable), we also plan a modeling part where the aim is to find which residue we would have to mutate in order to have a stable protein after the switch.
 
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The advantage of such a system is that we could apply the light on a system and then remove it (not like if we added some liquid on the cells).
 
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== '''Where we are from''' ==
== '''Where we are from''' ==
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We are all studying at EPFL aka ''the Swiss Institute of Technology of Lausanne''. The campus is located near the shore of lake Léman in the surroundings of the city of Lausanne, 50 km away from Geneva.
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We are all studying at EPFL aka ''the Swiss Institute of Technology of Lausanne''. The campus is located near the shore of lake Léman in the surroundings of the city of Lausanne, 50 km away from Geneva.<br><br><br>
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!align="center"|[[Team:EPF-Lausanne|Home]]
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!align="center"|[[Team:EPF-Lausanne/Team|The Team]]
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Latest revision as of 18:58, 21 October 2009



Contents








Team



Who we are

We are a team of undergraduates with an interdisciplinary background. Most of us are from the School of Life Sciences but some are from microengineering, mechanical engineering and chemistry.

Group : from left to right : up : Nicolas G, Christian, Gabriela, Tú, Nathalie, Carine, Nicolas D, Rafael, down : Heidi, Sebastian, Caroline, Mélanie


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UNDERGRADS

Christian Adamczyk

Caroline Baer

Nathalie Brandenberg

Heidi Fournier

Nicolas Gobet

Mélanie Guittet or the official agarose gel loader

Tú Nguyen

Rafael Pennese

Gabriela Pregernig

Basile Wicky or the official LB/Agar plate bubble cleaner




INSTRUCTORS

Nicolas Dénervaud

Carine Gubelmann

Marco Stenta

Enrico Spiga



ADVISORS

Bart Deplancke

Sebastian Maerkl

Matteo Dal Peraro


Where we are from

We are all studying at EPFL aka the Swiss Institute of Technology of Lausanne. The campus is located near the shore of lake Léman in the surroundings of the city of Lausanne, 50 km away from Geneva.






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