Team:uOttawa/Parts

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         <a href="https://2009.igem.org/Team:uOttawa/Project">Project</a></li>
         <a href="https://2009.igem.org/Team:uOttawa/Project">Project</a></li>
         <li>
         <li>
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         <a href="https://2009.igem.org/Team:uOttawa/Parts">Parts</a></li>
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         <a href="https://2009.igem.org/Team:uOttawa/Biobricks">Biobricks</a></li>
         <li>
         <li>
         <a href="https://2009.igem.org/Team:uOttawa/Modeling">Modeling</a></li>
         <a href="https://2009.igem.org/Team:uOttawa/Modeling">Modeling</a></li>
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<a href="https://2009.igem.org/Team:uOttawa/Notebook">Notebook</a></li>
<a href="https://2009.igem.org/Team:uOttawa/Notebook">Notebook</a></li>
       <li>
       <li>
 +
        <li> <a href="https://2009.igem.org/Team:uOttawa/Acknowlegments">Acknowledgments</a></li>
         <a href="https://2009.igem.org/Team:uOttawa/Project"">FR</a></li>
         <a href="https://2009.igem.org/Team:uOttawa/Project"">FR</a></li>
<li>
<li>
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     </div>
     </div>
     <h1 id="header">
     <h1 id="header">
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       <a href="/">uOttawa IGEM2009</a></h1>
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       uOttawa IGEM2009</h1>
     <div id="wrap">
     <div id="wrap">
       <div id="page">
       <div id="page">
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         <h2>The Project</h2>
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         <h2>Biobrick </h2>
         <p>
         <p>
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          'With more and easier access to high-calorie
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We have contributed to the iGEM biobrick registry by submitting several parts which can be used in eukaryotic organisms, namely in Yeast and Mammalian cells. The Gal 1/10 divergent promoter is essentially composed of two promoters and is capable of driving gene expression bidirectionally. Gene expression is driven in one direction by the Gal 1 promoter and by the Gal 10 promoter in the other direction, both promoters are inducible by galactose present in the cell medium. The construct was isolated from the pRS4T123 plasmid, generously donated to us by the James Collins Lab. Due  to it's divergent design, the dual promoter is compact and thus easy to work with. Hilary Phenix and Vida Adebi helped us with flow cytometry and they also generated a yeast strain containing a Gal 1/10 divergent promoter, with Gal 10 driving GFP expression. We characterized the Gal 10 component of the promoter in yeast by performing a dose response experiment where we induced the yeast with various concentrations of galactose and measured the resulting GFP output using a flow cytometer. We also contributed a CYC1 terminator containing a stop codon to the registry, a commonly used terminator sequence in yeast which was also isolated form the pRS4T123 plasmid . Including a stop codon with the terminator allows this construct to be fused with proteins in the biofusion format (assembly standard 23). We also submitted a CMV promoter, naturally found in the Cytomegalovirus. The submitted promoter was isolated from a plasmid that was created in our lab, pCMV is a constitutive promoter commonly used in mammalian cloning vectors. We submitted these parts in a effort provide at least some basic components to the registry that can be used by future iGEM teams who wish to engineer eukaryotic cells
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foods the worldwide prevalence of obesity has
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been on the rise in the past quarter century. In
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Canada alone, the average rate of obesity has
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doubled from 1979 to 2004, and approximately
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23% of Canadian adults are obese (As estimated
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by Statistics Canada). Obesity is typically
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associated with many adverse health conditions
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and puts an enormous strain on the public
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healthcare system. Our goal is to engineer a
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strain of Lacobacillus to express the enzymes
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required for the synthesis of cellulose from glucose
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in an attempt to reduce the caloric intake
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of obese individuals. Cellulose is a polymer of
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linked D-glucose units that cannot be digested
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by humans. Lactobacillus is a strain of bacteria,
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which is commonly used to make yogurt,
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cheese, beer and other fermented foods, and
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is in fact part of the natural human gut fl ora.
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The idea would be to have an obese individual
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ingest yogurt containing our engineered strain
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of bacteria as a probiotic. That way after they
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have consumed their meal, a portion of the
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glucose from the meal would be converted into
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cellulose in the intestines, effectively reducing
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their caloric intake.
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        </p>
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        <p class="info">
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          |          <a href="#">March 26th</a>
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        </p>
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        <h2>Lorem ipsum</h2>
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        <p>
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          Li Europan lingues es membres del sam familie. Lor separat existentie es un myth. Por scientie, musica, sport etc, li tot Europa usa li sam vocabularium. Li lingues differe solmen in li grammatica, li pronunciation e li plu commun vocabules. Omnicos directe al desirabilita; de un nov lingua franca: on refusa continuar payar custosi traductores. It solmen va esser necessi far uniform grammatica, pronunciation e plu sommun paroles.
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        </p>
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        <p>
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          Ma quande lingues coalesce, li grammatica del resultant lingue es plu simplic e regulari quam ti del coalescent lingues. Li nov lingua franca va esser plu simplic e regulari quam li existent Europan lingues. It va esser tam simplic quam Occidental: in fact, it va esser Occidental. A un Angleso it va semblar un simplificat Angles, quam un skeptic Cambridge amico dit me que Occidental es.
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        </p>
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        <p class="info">
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          |          <a href="#">March 26th</a>
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        </p>
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        <h2>Lorem ipsum</h2>
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          Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut laoreet dolore magna aliquam erat volutpat. Ut wisi enim ad minim veniam, quis nostrud exerci tation ullamcorper suscipit lobortis nisl ut aliquip ex ea commodo consequat. Duis autem vel eum iriure dolor in hendrerit in vulputate velit esse molestie consequat, vel illum dolore eu feugiat nulla facilisis at vero eros et accumsan et iusto odio dignissim qui blandit praesent luptatum zzril delenit augue duis dolore te feugait nulla facilisi.
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        </p>
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        <p>
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          Epsum factorial non deposit quid pro quo hic escorol. Olypian quarrels et gorilla congolium sic ad nauseum. Souvlaki ignitus carborundum e pluribus unum. Defacto lingo est igpay atinlay. Marquee selectus non provisio incongruous feline nolo contendre. Gratuitous octopus niacin, sodium glutimate. Quote meon an estimate et non interruptus stadium. Sic tempus fugit esperanto hiccup estrogen. Glorious baklava ex librus hup hey ad infinitum. Non sequitur condominium facile et geranium incognito. Epsum factorial non deposit quid pro quo hic escorol. Marquee selectus non provisio incongruous feline nolo contendre Olypian quarrels et gorilla congolium sic ad nauseum. Souvlaki ignitus carborundum e pluribus unum.
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        </p>
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        <p class="info">
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          |<a href="#">March 26th</a>
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         </p>
         </p>
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<p> </p>
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       </div>
       </div>
       <hr class="hide" />
       <hr class="hide" />
       <div id="sidebar">
       <div id="sidebar">
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         <h3>Previous Posts</h3>
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         <h3>uOttawa iGEM2009</h3>
         <ul>
         <ul>
           <li>
           <li>
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           <a href="#">Lorem ipsum</a></li>
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           <a href="http://www.ipm-int.org/boxmode/pdf/Ethics.pdf" target="_parent">ETHICS</a></li>
           <li>
           <li>
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           <a href="#">Lorem ipsum</a></li>
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           <a href="http://www.ipm-int.org/boxmode/pdf/Security.pdf">SECURITY</a></li>
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          <a href="#">Lorem ipsum</a></li>
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          <a href="#">Lorem ipsum</a></li>
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         </ul>
         </ul>
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         <h3>Links</h3>
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<h3>Health</h3>
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         <ul>
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          <li>  <a href="http://www.ipm-int.org/boxmode/pdf/Probiotics in the Food Industry.pdf" target="_parent">probiotics</a></li>
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          <li>  <a href="http://www.ipm-int.org/boxmode/pdf/Acetobacter_xylinum.pdf">Acetobacter xylinum</a></li>
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          <li>  <a href="http://www.ipm-int.org/boxmode/pdf/Obesity.pdf">Obesity</a></li>
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        </ul>
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      <h3>Sponsors</h3>
         <ul>
         <ul>
           <li>
           <li>
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           <a href="http://www.ukthoughts.co.uk/">UK Thoughts</a></li>
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           <a href="http://www.uottawa.ca">uottawa</a></li>
           <li>
           <li>
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           <a href="#">Link Item 2</a></li>
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           <a href="http://www.medicine.uottawa.ca/">Faculty of medicine</a></li>
           <li>
           <li>
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           <a href="#">Link Item 3</a></li>
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           <a href="http://www.engineering.uottawa.ca/">Faculty of engineering</a></li>
           <li>
           <li>
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           <a href="#">Link Item 4</a></li>
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           <a href="http://www.uottawa.ca/research/">VP research</a></li>
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          <li>
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          <a href="http://www.epocal.com/">Epocal</a></li>
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          <li>
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          <a href="http://www.medicine.uottawa.ca/crem/eng/">CREM-CRME</a></li>
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          <li>
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          <a href="http://www.gehealthcare.com/caen/">GE healthcare</a></li>
 +
         
         </ul>
         </ul>
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         <h3>Bits</h3>
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         <h3>Latest Events</h3>
         <ul id="bits">
         <ul id="bits">
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          <li>Lorem ipsum dolor sit amet, consectetuer adipiscing elit</li>
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<li>the waterloo getogether with other ontario teams</li>        
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          <li>Lorem ipsum dolor sit amet, consectetuer adipiscing elit</li>
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<li>tech museum on the 5th of May</li>
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           <li>Lorem ipsum dolor sit amet, consectetuer adipiscing elit</li>
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           <li>Day one, where do we start</li>
         </ul>
         </ul>
       </div>
       </div>
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     <div id="footer">
     <div id="footer">
       <p>
       <p>
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         &copy; 2006 Site Title, all rights reserved.<br />
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         &copy; 2009 uOttawa iGEM, all rights reserved.<br />
         Powered by  
         Powered by  
         <a href="http://www.example.com/" title="Powered by .."> uottawa igem2009</a>,  
         <a href="http://www.example.com/" title="Powered by .."> uottawa igem2009</a>,  
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
 
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook.  PLEASE keep all of your pages within your teams namespace. 
 
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|-
 
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
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|align="center"|[[Team:uOttawa | Team Example]]
 
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
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!align="center"|[[Team:uOttawa|Home]]
 
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!align="center"|[[Team:uOttawa/Team|The Team]]
 
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!align="center"|[[Team:uOttawa/Project|The Project]]
 
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!align="center"|[[Team:uOttawa/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:uOttawa/Modeling|Modeling]]
 
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!align="center"|[[Team:uOttawa/Notebook|Notebook]]
 
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(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
 
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===Note===
 
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If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here.  This is not necessary but it may be a nice list to keep track of.
 

Latest revision as of 19:03, 21 October 2009

uOttawa IGEM2009

uOttawa IGEM2009

Biobrick

We have contributed to the iGEM biobrick registry by submitting several parts which can be used in eukaryotic organisms, namely in Yeast and Mammalian cells. The Gal 1/10 divergent promoter is essentially composed of two promoters and is capable of driving gene expression bidirectionally. Gene expression is driven in one direction by the Gal 1 promoter and by the Gal 10 promoter in the other direction, both promoters are inducible by galactose present in the cell medium. The construct was isolated from the pRS4T123 plasmid, generously donated to us by the James Collins Lab. Due to it's divergent design, the dual promoter is compact and thus easy to work with. Hilary Phenix and Vida Adebi helped us with flow cytometry and they also generated a yeast strain containing a Gal 1/10 divergent promoter, with Gal 10 driving GFP expression. We characterized the Gal 10 component of the promoter in yeast by performing a dose response experiment where we induced the yeast with various concentrations of galactose and measured the resulting GFP output using a flow cytometer. We also contributed a CYC1 terminator containing a stop codon to the registry, a commonly used terminator sequence in yeast which was also isolated form the pRS4T123 plasmid . Including a stop codon with the terminator allows this construct to be fused with proteins in the biofusion format (assembly standard 23). We also submitted a CMV promoter, naturally found in the Cytomegalovirus. The submitted promoter was isolated from a plasmid that was created in our lab, pCMV is a constitutive promoter commonly used in mammalian cloning vectors. We submitted these parts in a effort provide at least some basic components to the registry that can be used by future iGEM teams who wish to engineer eukaryotic cells

Retrieved from "http://2009.igem.org/Team:uOttawa/Parts"