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From 2009.igem.org

Revision as of 19:05, 21 October 2009 (view source) Abrahamavelar (Talk | contribs) (New page: To ligate two biobricks into a base vector. Part 1: 2 µl Part 2: 2 µl Destination Plasmid: 2 µl 10X T4 Ligase Buffer: 2 µl T4 Ligase: 1 µl H2O: 11 µl Incubate at room tempera...) ← Older edit		Revision as of 19:08, 21 October 2009 (view source) Abrahamavelar (Talk | contribs) Newer edit →
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	To ligate two biobricks into a base vector.		To ligate two biobricks into a base vector.
-		+	<br><br>
-	Part 1: 2 µl	+	Part 1: 2 µl<br>
-	Part 2: 2 µl	+	Part 2: 2 µl<br>
-	Destination Plasmid: 2 µl	+	Destination Plasmid: 2 µl<br>
-	10X T4 Ligase Buffer: 2 µl	+	10X T4 Ligase Buffer: 2 µl<br>
-	T4 Ligase: 1 µl	+	T4 Ligase: 1 µl<br>
-	H2O: 11 µl	+	H2O: 11 µl<br>
	Incubate at room temperature for 10 minutes and then inactivate the enzyme at 80°C for 20 minutes.		Incubate at room temperature for 10 minutes and then inactivate the enzyme at 80°C for 20 minutes.
	Transform 5 μl of the ligation product into 50 μl of competent DH5-alpha E. coli cells.		Transform 5 μl of the ligation product into 50 μl of competent DH5-alpha E. coli cells.
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Revision as of 19:08, 21 October 2009

Part 1: 2 µl
Part 2: 2 µl
Destination Plasmid: 2 µl
10X T4 Ligase Buffer: 2 µl
T4 Ligase: 1 µl
H2O: 11 µl

Incubate at room temperature for 10 minutes and then inactivate the enzyme at 80°C for 20 minutes.

Transform 5 μl of the ligation product into 50 μl of competent DH5-alpha E. coli cells.

Retrieved from "http://2009.igem.org/Team:LCG-UNAM-Mexico/Resources/Protocols/Ligation"