"
Team:DTU Denmark/notebookredoxilator
From 2009.igem.org
(Difference between revisions)
| Line 188: | Line 188: | ||
<font size="4"><b>Activities relating to the redoxilator</b></font><br><br> | <font size="4"><b>Activities relating to the redoxilator</b></font><br><br> | ||
| + | |||
| + | <font size="3"><b> Thursday 15/10/2009 </b></font><br> | ||
| + | BioLector experiment initiated! Lets get some results! | ||
| + | <br><br> | ||
| + | |||
| + | <font size="3"><b> Wednesday 14/10/2009 </b></font><br> | ||
| + | Colonies were picked from plate to grow to have inoculum for the BioLector experiment tomorrow | ||
| + | <br><br> | ||
| + | |||
| + | <font size="3"><b> Monday 5/10/2009 </b></font><br> | ||
| + | Yeast was transferred to new plate to be sure we have a real transformant | ||
| + | <br><br> | ||
| + | |||
| + | <font size="3"><b> Thursday 1/10/2009 </b></font><br> | ||
| + | Construt and Leaky are ready for yeast transformation into CEN PK 113 5D (Ura-) using the URA marker on these plasmidss<br><br> | ||
| + | |||
| + | <font size="3"><b> Wednesday 30/9/2009 </b></font><br> | ||
| + | Purification of vector. Digestion with XmaI of leaky p241i was conducted (Should yield 2500 & 4500)<br><br> | ||
| + | |||
| + | <font size="3"><b> Tuesday 29/9/2009 </b></font><br> | ||
| + | Liquid over night colonies. Cultures were randomly picked using a sterile pipette tip from the transformation plate and incubated into liquid media containing kanamycin. <br><br> | ||
| + | |||
| + | |||
| + | <font size="3"><b> Monday 28/9/2009 </b></font><br> | ||
| + | The construct AKA p241i digested wiith NotI to remove the redoxilated. Following gelpurification the backbone with ligated and transformed into E coli<br><br> | ||
| + | |||
| + | |||
| + | <font size="3"><b> Friday 25/9/2009 </b></font><br> | ||
| + | Colonies seen!<br><br> | ||
| + | |||
| + | <font size="3"><b> Thursday 24/9/2009 </b></font><br> | ||
| + | Our dear redoxilator construct arrieved from California (DNA 2.0) | ||
| + | Dna extracted from filter paper and transformed into E Coli | ||
Revision as of 20:32, 21 October 2009
| Home | The Team | The Project | Parts submitted | Modelling | Notebook |
|
Activities relating to our two sub-projects: - The Redoxilator - The USERTM assembly standard - Biobricks - Protocols |
Day-to-day activities Activities relating to the redoxilator Thursday 15/10/2009 BioLector experiment initiated! Lets get some results! Wednesday 14/10/2009 Colonies were picked from plate to grow to have inoculum for the BioLector experiment tomorrow Monday 5/10/2009 Yeast was transferred to new plate to be sure we have a real transformant Thursday 1/10/2009 Construt and Leaky are ready for yeast transformation into CEN PK 113 5D (Ura-) using the URA marker on these plasmidss Wednesday 30/9/2009 Purification of vector. Digestion with XmaI of leaky p241i was conducted (Should yield 2500 & 4500) Tuesday 29/9/2009 Liquid over night colonies. Cultures were randomly picked using a sterile pipette tip from the transformation plate and incubated into liquid media containing kanamycin. Monday 28/9/2009 The construct AKA p241i digested wiith NotI to remove the redoxilated. Following gelpurification the backbone with ligated and transformed into E coli Friday 25/9/2009 Colonies seen! Thursday 24/9/2009 Our dear redoxilator construct arrieved from California (DNA 2.0) Dna extracted from filter paper and transformed into E Coli |
Work process Our team works parallel in smaller sub-teams. Some of us work hard in the lab, while others are in the process of developing software and the in silico model of the Redoxilator system. However, we constantly keep each other updated, and meet often to exchange ideas and take turns at the different tasks, thus exhausting all of our combined knowledge in every aspect of this project. |
| Comments or questions to the team? Please Email us -- Comments of questions to webmaster? Please Email us |
