Team:Paris/Transduction overview2 strategy

From 2009.igem.org

(Difference between revisions)
(New page: ===B. Our Strategy=== <html> </div> <div id="paris_content_boxtop"> </div> <div id="paris_content"> </html> ====B.1 The Fec operon ==== Few ABC transporter such as FecABCD (iron transpo...)
(→Our strategy: The Fec operon)
 
(13 intermediate revisions not shown)
Line 1: Line 1:
-
===B. Our Strategy===
+
<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Transduction_overview#top | Receiving the message]] > [[Team:Paris/Transduction_overview#bottom | Our strategy]]
 +
{{Template:Paris2009}}
 +
{{Template:Paris2009_menu4}}
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
==Our strategy: The Fec operon ==
<html>
<html>
 +
<style type="text/css">
 +
#left-side {
 +
    position: absolute;
 +
    height: 23px;
 +
    width: 30px;
 +
    top: 0px;
 +
    left: 150px;
 +
    margin-top:10px;
 +
    padding-top: 7px;
 +
    background: url(https://static.igem.org/mediawiki/2009/1/1b/Left_menu_pari.png);
 +
    z-index:4;
 +
}
 +
 +
#middle-side {
 +
    height: 25px;
 +
    width: 370px;
 +
    position: absolute;
 +
    top: 0px;
 +
    left: 160px;
 +
    margin-top:10px;
 +
    padding-top: 5px;
 +
    background: #dadada;
 +
    z-index:5;
 +
}
 +
 +
#right-side {
 +
    position: absolute;
 +
    height: 23px;
 +
    width: 30px;
 +
    margin-top:10px;
 +
    padding-top: 7px;
 +
    top: 0px;
 +
    left: 510px;
 +
    background: url(https://static.igem.org/mediawiki/2009/4/40/Right_menu_paris.png);
 +
    z-index:4;
 +
}
 +
 +
#left-side2 {
 +
    position: absolute;
 +
    height: 23px;
 +
    width: 30px;
 +
    top: 35px;
 +
    left: 175px;
 +
    margin-top:10px;
 +
    padding-top: 7px;
 +
    background: url(https://static.igem.org/mediawiki/2009/4/40/Left_menu_paris2.png);
 +
    z-index:4;
 +
}
 +
 +
#middle-side2 {
 +
    height: 25px;
 +
    width: 310px;
 +
    position: absolute;
 +
    top: 35px;
 +
    left: 185px;
 +
    margin-top:10px;
 +
    padding-top: 5px;
 +
    background: white;
 +
    z-index:5;
 +
}
 +
 +
#right-side2 {
 +
    position: absolute;
 +
    height: 23px;
 +
    width: 30px;
 +
    margin-top:10px;
 +
    padding-top: 7px;
 +
    top: 35px;
 +
    left: 490px;
 +
    background: url(https://static.igem.org/mediawiki/2009/f/f8/Right_menu_paris2.png);
 +
    z-index:4;
 +
}
 +
 +
a.menu_sub {
 +
    padding-left: 7px;
 +
    padding-right: 7px;
 +
}
 +
 +
a.menu_sub_active {
 +
    padding-left: 7px;
 +
    padding-right: 7px;
 +
    color:#b0310e;
 +
    font-weight:bold;
 +
}
 +
</style>
 +
<div id="left-side"></div>
 +
<div id="middle-side"><center>
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Transduction_overview2#bottom"> Main </a>|
 +
<a class="menu_sub" href="https://2009.igem.org/Team:Paris/Transduction_overview2_transduction#bottom"> Transduction</a>|
 +
<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Transduction_overview2_strategy#bottom"> Our strategy</a>|
 +
<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Transduction_overview2_construction#bottom"> Construction</a>
 +
</center>
</div>
</div>
-
<div id="paris_content_boxtop">
+
<div id="right-side"></div>
 +
<div id="left-side2"></div>
 +
<div id="middle-side2"><center>
 +
<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Transduction_overview2_strategy#Our_strategy:_The_Fec_operon"> The Fec operon</a>|
 +
<a class="menu_sub" href="https://2009.igem.org/Team:Paris/Transduction_overview2_strategy#Our_strategy:_The_trick_TCS"> The trick TCS</a>
 +
</center>
</div>
</div>
-
<div id="paris_content">
+
<div id="right-side2"></div>
</html>
</html>
-
 
-
====B.1 The Fec operon ====
 
Few ABC transporter such as FecABCD (iron transporter) are able to induce a response regardless of the tranlocation, due to the activity of FecA, moreover some mutant can also have a constitutive expression of FecABCD .
Few ABC transporter such as FecABCD (iron transporter) are able to induce a response regardless of the tranlocation, due to the activity of FecA, moreover some mutant can also have a constitutive expression of FecABCD .
Line 22: Line 127:
'''problems :''' the message is unidirectionnal and unrepeatable. It would just be a proof of principle that a vesicle-mediated controlled communication is possible.
'''problems :''' the message is unidirectionnal and unrepeatable. It would just be a proof of principle that a vesicle-mediated controlled communication is possible.
 +
<html>
 +
</div>
 +
<div id="paris_content_boxtop">
 +
</div>
 +
<div id="paris_content">
 +
</html>
-
====B.2 The trick TCS====
+
==Our strategy: The trick TCS==
We could ,with the help of Alfonso Jaramillo, design a synthetic PBP which could detect the substrate we choosed and activate a specific HK.
We could ,with the help of Alfonso Jaramillo, design a synthetic PBP which could detect the substrate we choosed and activate a specific HK.
Line 29: Line 140:
Even if the idea of creating a synthethic protein was very attractive, it would have been difficult to have concrete result in just 3 months.
Even if the idea of creating a synthethic protein was very attractive, it would have been difficult to have concrete result in just 3 months.
 +
 +
 +
{{Template:Paris2009_guided|Transduction_overview2_transduction#bottom|Ethics_overview#top}}

Latest revision as of 20:47, 21 October 2009

iGEM > Paris > Receiving the message > Our strategy




Our strategy: The Fec operon

Few ABC transporter such as FecABCD (iron transporter) are able to induce a response regardless of the tranlocation, due to the activity of FecA, moreover some mutant can also have a constitutive expression of FecABCD .


The plan would be to use FecA- mutant receiver and FecA+ mutant donor to transfert the constitutive FecA protein to the receiver. In this case the receiver will express the FecABCD operon without being induce by ferric citrate in the medium , and so we could place under the control of the Fec ABCD promoter, which is called pfec, the gene sequence encoding for the response. For the moment a response that would be easy to detect is the fluorescence of the RFP and the biobrick BBa-J61002 is the perfect candidate to test the system.


We also discovered that some fecR and fecI mutants can be use to amplify the signal because they have a constitutive activity. So we put under the control of pfec a FecR and FecI mutated. When they will be expressed, they will be activators of pfec and consequently of RFP. Normaly we would be able to obtain a increasing fluorescence.


fec operon induction


problems : the message is unidirectionnal and unrepeatable. It would just be a proof of principle that a vesicle-mediated controlled communication is possible.

Our strategy: The trick TCS

We could ,with the help of Alfonso Jaramillo, design a synthetic PBP which could detect the substrate we choosed and activate a specific HK. The previous work done by Valencia in 2006 was to design vanilin-sensitive PBP and a network for a graduated response whereas we just need a proteic sensitive PBP and a binary type of response.

Even if the idea of creating a synthethic protein was very attractive, it would have been difficult to have concrete result in just 3 months.


Open book.gif

← Previous - Next →

Retrieved from "http://2009.igem.org/Team:Paris/Transduction_overview2_strategy"