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Team:DTU Denmark/notebookredoxilator
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<font size="3"><b> Wednesday 14/10/2009 </b></font><br> | <font size="3"><b> Wednesday 14/10/2009 </b></font><br> | ||
| - | + | To have inoculum for the BioLector experiment, colonies were picked from our plates and grown till tomorrow. | |
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<font size="3"><b> Monday 5/10/2009 </b></font><br> | <font size="3"><b> Monday 5/10/2009 </b></font><br> | ||
| - | + | The yeasts were streak diluted onto a new plate to be sure that we have a single transformant. | |
<br><br> | <br><br> | ||
<font size="3"><b> Thursday 1/10/2009 </b></font><br> | <font size="3"><b> Thursday 1/10/2009 </b></font><br> | ||
| - | + | Our redoxilator construct and <i>leaky</i> construct were ready for yeast transformation into CEN PK 113 5D (Ura-) using the URA marker present these plasmids<br><br> | |
<font size="3"><b> Wednesday 30/9/2009 </b></font><br> | <font size="3"><b> Wednesday 30/9/2009 </b></font><br> | ||
| - | + | The resulting vectors were purification. The presence of our leaky redoxilator construct was verified by digestion with XmaI<br><br> | |
<font size="3"><b> Tuesday 29/9/2009 </b></font><br> | <font size="3"><b> Tuesday 29/9/2009 </b></font><br> | ||
| - | Liquid over night colonies. Cultures were randomly picked using a sterile pipette tip from the transformation plate and incubated into liquid media containing kanamycin. <br><br> | + | Liquid over night colonies were prepared. Cultures were randomly picked using a sterile pipette tip from the transformation plate and incubated into liquid media containing kanamycin. <br><br> |
<font size="3"><b> Monday 28/9/2009 </b></font><br> | <font size="3"><b> Monday 28/9/2009 </b></font><br> | ||
| - | + | To test how leaky our redoxilator construct was, the redoxilator part was removed by NotI digested followed by gel-purification. The backbone missing the redox sensor/activator was ligated and transformed into <i>E. coli</i><br><br> | |
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<font size="3"><b> Thursday 24/9/2009 </b></font><br> | <font size="3"><b> Thursday 24/9/2009 </b></font><br> | ||
Our dear redoxilator construct arrieved from California (DNA 2.0) | Our dear redoxilator construct arrieved from California (DNA 2.0) | ||
| - | + | The DNA was extracted from filter paper using MilliQ water and transformed into <i>E. Coli</i> | |
Revision as of 20:58, 21 October 2009
| Home | The Team | The Project | Parts submitted | Modelling | Notebook |
|
Activities relating to our two sub-projects: - The Redoxilator - The USERTM assembly standard - Biobricks - Protocols |
Day-to-day activities Activities relating to the redoxilator Thursday 15/10/2009 BioLector experiment initiated! Lets get some results! Wednesday 14/10/2009 To have inoculum for the BioLector experiment, colonies were picked from our plates and grown till tomorrow. Monday 5/10/2009 The yeasts were streak diluted onto a new plate to be sure that we have a single transformant. Thursday 1/10/2009 Our redoxilator construct and leaky construct were ready for yeast transformation into CEN PK 113 5D (Ura-) using the URA marker present these plasmids Wednesday 30/9/2009 The resulting vectors were purification. The presence of our leaky redoxilator construct was verified by digestion with XmaI Tuesday 29/9/2009 Liquid over night colonies were prepared. Cultures were randomly picked using a sterile pipette tip from the transformation plate and incubated into liquid media containing kanamycin. Monday 28/9/2009 To test how leaky our redoxilator construct was, the redoxilator part was removed by NotI digested followed by gel-purification. The backbone missing the redox sensor/activator was ligated and transformed into E. coli Friday 25/9/2009 Colonies seen! Thursday 24/9/2009 Our dear redoxilator construct arrieved from California (DNA 2.0) The DNA was extracted from filter paper using MilliQ water and transformed into E. Coli |
Work process Our team works parallel in smaller sub-teams. Some of us work hard in the lab, while others are in the process of developing software and the in silico model of the Redoxilator system. However, we constantly keep each other updated, and meet often to exchange ideas and take turns at the different tasks, thus exhausting all of our combined knowledge in every aspect of this project. |
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