Team:UNICAMP-Brazil/Yeastguard/Killing

From 2009.igem.org

(Difference between revisions)
Line 2: Line 2:
__NOTOC__
__NOTOC__
=The Yeastguard: Killing=
=The Yeastguard: Killing=
-
<p style=”text-align:justify;”></p>
+
<p style=”text-align:justify;”>The killing mechanism was designed based on the contaminants´ features. The lactobacilli are gram-positive bacteria and consequently have specific compounds in the cell wall. In addition, lysozyme is a protein that acts on the cell wall of gram-positive bacteria, catalyzing the hydrolysis of the peptidoglycan. Taking this into account, we decided to use the Lysozyme as a way to kill the contaminants and not cause injury to the yeasts.
 +
The link between the recognition and killing mechanisms is the device that consists of one of the sensitive lactate promoters and the lysozyme.</p>
==Killing protein: Lysozyme==
==Killing protein: Lysozyme==

Revision as of 21:25, 21 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif

The Yeastguard: Killing

The killing mechanism was designed based on the contaminants´ features. The lactobacilli are gram-positive bacteria and consequently have specific compounds in the cell wall. In addition, lysozyme is a protein that acts on the cell wall of gram-positive bacteria, catalyzing the hydrolysis of the peptidoglycan. Taking this into account, we decided to use the Lysozyme as a way to kill the contaminants and not cause injury to the yeasts. The link between the recognition and killing mechanisms is the device that consists of one of the sensitive lactate promoters and the lysozyme.

Killing protein: Lysozyme