Team:Freiburg bioware/Human Practice/Safety

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Safety<span class="art-PostHeader"></span> </h2>
Safety<span class="art-PostHeader"></span> </h2>
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===Safety===
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<div style="text-align: center;"></div>
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<br />
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About: Safety in the Lab<br>
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<p><H4>About: Safety in the Lab </H4></br><br>
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</p>
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1.Would any of our project ideas raise safety issues in terms of:<br>
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<p><b>1.Would any of our project ideas raise safety issues in terms
-
 
+
of:<br />
-
-researcher safety,<br>
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</p>
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-public safety, or<br>
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<p>-researcher safety,<br />
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-environmental safety?<br>
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-public safety, or<br />
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-environmental safety?<br /></b>
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Standards for a Security Level 1 lab:<br>  
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</p>
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<p>Standards for a Security Level 1 lab:<br />
-
The lab where our iGEM team works, is a security level 1 lab according to the Gentechnikgesetz (law for gene technology). This means that the lab conforms to standards that our instructors introduced us to in the beginning of the project in April 2009. After a safety briefing for the group, we had a “safety-tour” through the lab while our advisor Kristian told us how to handle the different instruments like centrifuges and Bunsen burners and showed us were to find the fire extinguishers, safety showers and eye showers and explained the handling. In the lab we store highly inflammable materials and gas containers in a safety cabinet.
+
</p>
-
When we were working with hazardous chemicals or genetically modified organisms we were wearing suitable protection gear such as lab coat, safety glasses and gloves.
+
<p>The lab where our iGEM team works, is a security level 1 lab
-
We disinfected our hands, the pipettes and the bench before and after working with bacteria and phages. Most of the material we use in the lab has to be sterile and it went through the autoclave before and after using. All the bacteria waste was collected in autoclave bags and autoclaved before delivered to the waste containers to prevent an environmental pollution.
+
according to the Gentechnikgesetz (law for gene technology). This means
-
While working with toxic stuff or reagents that are harmful to health we additionally used the hood as working station.  
+
that the lab conforms to standards that our instructors introduced us
-
When using liquid nitrogen or working with UV-light we were wearing safety glasses and gloves and handled our reagents with care.<br>
+
to at the beginning of the project in April 2009. After a safety
-
 
+
briefing for the group, we had a &ldquo;safety-tour&rdquo;
-
2. Is there a local biosafety group, committee, or review board at your institution?<br>
+
through the lab while
-
 
+
our adviser Kristian told us about the different chemicals we would be using, how to handle genetically modified bacteria and how to operate the different instruments
-
In Germany we have by law a safety officer in each lab/working group who controls their work and notes.<br>
+
like centrifuges and Bunsen burners. Furthermore, he showed us where to find the fire
-
 
+
extinguishers, safety showers and eye showers and explained their
-
3. What does your local biosafety group think about your project?<br>
+
handling. In the lab, we store highly inflammable materials and gas
-
 
+
containers in a safety cabinet.
-
We made sure to follow all regulations described by the Gentechnikgesetz (law for gene technology) including the Gentechnik-Sicherheitsverordnung-GenTSV (gene technology safety-regulation), the Gentechnik-Aufzeichnungsverordnung-GenTAufzV (gene technology recording regulation) and the Gentechnik-Verfahrensverordnung-GenTVfV (gene technology proceeding regulation).
+
When we were working with hazardous chemicals or genetically modified
-
Thus we classified our work as safety-level one work.<br>
+
organisms we were wearing suitable protection gear such as lab coat,
-
 
+
safety glasses and gloves.
-
4. Do any of the new BioBrick parts that you made this year raise any safety issues?<br>
+
We disinfected our hands, the pipettes and the bench before and after
-
 
+
working with bacteria and phages. Most of the material we use in the
-
No, the E. coli strains with which we did our experiments have been deactivated, so that they aren’t anymore able to cause diseases. The E.coli strains are non pathogenic and non adherent. The genes we clone are coding for non toxic and non pathogenic proteins.<br>
+
lab has to be sterile and was autoclaved before using. All the bacteria waste as well as all consumable that had been in contact with bacteria were collected in autoclave bags or containers and
-
 
+
autoclaved before delivered to the waste containers to prevent an
-
 
+
environmental contamination.
-
 
+
While working with toxic stuff or reagents that are harmful to health,
-
 
+
we additionally used a hood as working station. When using liquid
 +
nitrogen or working with UV-light, we were wearing safety glasses and
 +
gloves and handled our reagents with care.<br /><br>
 +
</p>
 +
<p><b>2. Is there a local biosafety group, committee, or review
 +
board at your institution?</b><br />
 +
</p>
 +
<p>In Germany, we have by law a safety officer in each lab/working
 +
group who controls the work as well as the lab notes.<br /><br>
 +
</p>
 +
<p><b>3. What does your local biosafety group think about your
 +
project?</b><br />
 +
</p>
 +
<p>We made sure to follow all regulations described in the
 +
Gentechnikgesetz (law for gene technology) including the
 +
Gentechnik-Sicherheitsverordnung-GenTSV (gene technology
 +
safety-regulation), the Gentechnik-Aufzeichnungsverordnung-GenTAufzV
 +
(gene technology recording regulation) and the
 +
Gentechnik-Verfahrensverordnung-GenTVfV (gene technology proceeding
 +
regulation).
 +
Thus, we classified our work as safety-level one work.<br /><br>
 +
</p>
 +
<p><b>4. Do any of the new BioBrick parts that you made this year
 +
raise any safety issues?</b><br />
 +
</p>
 +
No, the <i>E. coli</i> strains, which we used for our experiments, have
 +
been deactivated, so that they aren&rsquo;t anymore able to cause any contamination.
 +
Importantly, the <i>E. coli</i> strains we used are all non-pathogenic and non-adherent strains. The genes we
 +
cloned are coding for non-toxic and non-pathogenic proteins.
 +
</div>
 +
<div class="cleared"></div>
 +
</div>
 +
</div>
 +
</div>
 +
<br />
 +
</div>
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</div>
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<div class="cleared"></div>
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<div class="art-Footer-text">
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Latest revision as of 21:42, 21 October 2009

FREiGEM

  Safety


About: Safety in the Lab



1.Would any of our project ideas raise safety issues in terms of:

-researcher safety,
-public safety, or
-environmental safety?

Standards for a Security Level 1 lab:

The lab where our iGEM team works, is a security level 1 lab according to the Gentechnikgesetz (law for gene technology). This means that the lab conforms to standards that our instructors introduced us to at the beginning of the project in April 2009. After a safety briefing for the group, we had a “safety-tour” through the lab while our adviser Kristian told us about the different chemicals we would be using, how to handle genetically modified bacteria and how to operate the different instruments like centrifuges and Bunsen burners. Furthermore, he showed us where to find the fire extinguishers, safety showers and eye showers and explained their handling. In the lab, we store highly inflammable materials and gas containers in a safety cabinet. When we were working with hazardous chemicals or genetically modified organisms we were wearing suitable protection gear such as lab coat, safety glasses and gloves. We disinfected our hands, the pipettes and the bench before and after working with bacteria and phages. Most of the material we use in the lab has to be sterile and was autoclaved before using. All the bacteria waste as well as all consumable that had been in contact with bacteria were collected in autoclave bags or containers and autoclaved before delivered to the waste containers to prevent an environmental contamination. While working with toxic stuff or reagents that are harmful to health, we additionally used a hood as working station. When using liquid nitrogen or working with UV-light, we were wearing safety glasses and gloves and handled our reagents with care.

2. Is there a local biosafety group, committee, or review board at your institution?

In Germany, we have by law a safety officer in each lab/working group who controls the work as well as the lab notes.

3. What does your local biosafety group think about your project?

We made sure to follow all regulations described in the Gentechnikgesetz (law for gene technology) including the Gentechnik-Sicherheitsverordnung-GenTSV (gene technology safety-regulation), the Gentechnik-Aufzeichnungsverordnung-GenTAufzV (gene technology recording regulation) and the Gentechnik-Verfahrensverordnung-GenTVfV (gene technology proceeding regulation). Thus, we classified our work as safety-level one work.

4. Do any of the new BioBrick parts that you made this year raise any safety issues?

No, the E. coli strains, which we used for our experiments, have been deactivated, so that they aren’t anymore able to cause any contamination. Importantly, the E. coli strains we used are all non-pathogenic and non-adherent strains. The genes we cloned are coding for non-toxic and non-pathogenic proteins.

contact:  freigem09@googlemail.com

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