Team:EPF-Lausanne

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==Concept==
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[[Image:LovTAP_dimer.png|right|300px|thumb|LovTAP dimer bound to DNA]]
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Recent discoveries of photoreceptors in many organisms got us excited about the possibility of using light-responsive genetic tools in synthetic biology. Indeed, such tools could in principle induce phenotypic changes in a more localized, preciser and faster fashion than currently available chemical-based methods. To evaluate the biotechnological potential of such tools, we specifically aimed to induce a change in gene expression, more specifically to directly turn a gene on or off, in a living organism, in response to a light stimulus.
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For this purpose, we used a light-sensitive DNA binding protein "LovTAP" (for Light, Oxygen, Voltage Tryptophan-Activated Protein) to convert a light input into a chosen output, here fluorescence generated by the RFP reporter gene.
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<span class="plainlinks">[{{SERVER}}{{localurl:Team:Alberta}} https://static.igem.org/mediawiki/2008/a/af/HOMEUAB.png]</span>
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Test graffiti using the Ellison account...
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The results clearly show that this light-induced gene switch tool works ''in vivo'', demonstrating the feasibility of implementing such powerful technology for a diverse range of bio(techno)logical applications.
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{| class="wikitable" style="text-align:center; width:100%;"
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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! scope=col | [[Image:Logo_MerckSerono.png|80 px]]
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! scope=col | [[Image:Logo_Novartis.png|100 px]]
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! scope=col | [[Image:Logo_UBS.jpg|200 px]]
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! scope=col | [[Image:Logo_ciba.jpg|80 px]]
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|align="center"|[[Team:EPF-Lausanne | Team Example]]
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! scope=col | [[Image:Logo_nikon.jpg|60 px]]
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! scope=col | [[Image:Logo_tecan.gif|80 px]]
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<!--- The Mission, Experiments --->
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!align="center"|[[Team:EPF-Lausanne|Home]]
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!align="center"|[[Team:EPF-Lausanne/Team|The Team]]
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<script type="text/javascript" src="http://clustrmaps.com/admin/3d/js/clustrmaps.js"></script>
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!align="center"|[[Team:EPF-Lausanne/Project|The Project]]
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!align="center"|[[Team:EPF-Lausanne/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:EPF-Lausanne/Modeling|Modeling]]
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!align="center"|[[Team:EPF-Lausanne/Notebook|Notebook]]
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!align="center"|[[Team:EPF-Lausanne/Lectures|Lectures]]
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</div><div CLASS="epfl09bouchon"></div>

Latest revision as of 22:45, 21 October 2009



Mainpage.jpg



Concept


LovTAP dimer bound to DNA

Recent discoveries of photoreceptors in many organisms got us excited about the possibility of using light-responsive genetic tools in synthetic biology. Indeed, such tools could in principle induce phenotypic changes in a more localized, preciser and faster fashion than currently available chemical-based methods. To evaluate the biotechnological potential of such tools, we specifically aimed to induce a change in gene expression, more specifically to directly turn a gene on or off, in a living organism, in response to a light stimulus.

For this purpose, we used a light-sensitive DNA binding protein "LovTAP" (for Light, Oxygen, Voltage Tryptophan-Activated Protein) to convert a light input into a chosen output, here fluorescence generated by the RFP reporter gene.

The results clearly show that this light-induced gene switch tool works in vivo, demonstrating the feasibility of implementing such powerful technology for a diverse range of bio(techno)logical applications.





Logo MerckSerono.png Logo Novartis.png Logo Syngenta.png Logo UBS.jpg Logo ciba.jpg Logo nikon.jpg Logo tecan.gif

Locations of visitors to this page

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