Team:Chiba/Project/Signaling-system

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:::::<Font Size="7">'''''E''.coli Time Manager '''</Font>  <html><a href="http://www.chiba-u.ac.jp/e/" target="_blank"><img src="https://static.igem.org/mediawiki/2009/5/5f/Logo_chiba-u.gif" align="right" width="250"></a></html>
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{| style="color:white;" cellpadding="2" cellspacing="0" border="0" width="100%" align="center" class="menu" |align="center"
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!align="center"|[[Team:Chiba|Home]]
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!align="center"|[[Team:Chiba/Team|The Team]]
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!align="center"|[[Team:Chiba/Parts|Parts]]
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!align="center"|[[Team:Chiba/Reference|Reference]]
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!align="center"|[[Team:Chiba/Notebook|Notebook]]
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!align="center"|[[Team:Chiba/Notebook/protocol|Protocols]]
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!align="center"|[[Team:Chiba/Links|Links]]
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!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
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!align="center"|[[Team:Chiba/Contact|Contact]]
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<table class="calendar" width="100%" border="1" cellpadding="1" cellspacing="0" align="center" bordercolor="#800000" align="center">
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'''[[Team:Chiba/Project|The Project]]'''
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'''1,''' [[Team:Chiba/Project#Introduction|Introduction]]
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'''2,''' [[Team:Chiba/Project#Project_Design|Project Design]]
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'''3,''' [[Team:Chiba/Project#Experiments,_Results_&_Discussion|Experiments, Results & Discussion]]
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3-1, [[Team:Chiba/Project#Making_LuxR_Mutants|Making LuxR Mutants]]
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3-2, [[Team:Chiba/Project#Characterization_of_LuxR_Mutants|Characterization of LuxR Mutants]]
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3-3, [[Team:Chiba/Project#Demonstration|Demonstration]]
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'''5,''' [[Team:Chiba/Project#Conclusions|Conclusions]]
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__NOTOC__
==== Signaling System ====
==== Signaling System ====
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*なんじゃこりゃーなおします。--[[User:Yoshimi|Yoshimi]] 13:22, 20 October 2009 (UTC)
 
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*sender使わないからいらないのでは？というか，cell-cell comunicationは使ってなくてただのtranscription activatorとしてluxRを使っているだけ...という面がつよい...ので，luxRの転写活性化がどのようにおこるか，をメインにしてはいかが?--[[User:Maiko|Maiko]] 13:43, 20 October 2009 (UTC)
 
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*次項のスクリーニングでsenderを使うので、ここでも図にSenderを入れておくべきだと考えます。--[[User:Yoshimi|Yoshimi]] 14:10, 20 October 2009 (UTC)
 
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*まあAHL合成用としてluxIが入った細胞は使うのだが，プロジェクト的にはcell-cell communicationは関係ないので，「センダー」「レシーバ」の概念をいれないほうが話の筋はとおる気が...？luxIは横でフツーに説明すればok。とおもいまいた。が，まあigem communityでluxR-luxIがわからない人はいないと思うので書いてもまあ問題はないかもしれません。 --[[User:Maiko|Maiko]] 14:27, 20 October 2009 (UTC)
 
[[Image:Chiba_quorumsensing.gif|600px]]
[[Image:Chiba_quorumsensing.gif|600px]]

Latest revision as of 22:55, 21 October 2009

E.coli Time Manager


Home The Team Parts Reference Notebook Protocols Links Acknowledgements Contact

The Project

1, Introduction

2, Project Design

3, Experiments, Results & Discussion

3-1, Making LuxR Mutants

3-2, Characterization of LuxR Mutants

3-3, Demonstration

5, Conclusions

Signaling System

Chiba quorumsensing.gif


In this project, we use acylated homoserine lactones (AHLs), signaling molecules used for [http://en.wikipedia.org/wiki/Quorum_sensing quorum sensing] in gram negative bacteria. Senders express LuxI or similar enzymes, which catalyze the production of AHLs, under the control of a constitutive (Tet) promoter. Each cell thus generates AHL more or less at a constant rate. AHL can freely permeate cell membranes and are detected by neighboring cells. Receivers constitutively express LuxR proteins (or a similar ortholog), the protein that detects AHL concentrations. When AHLs bind LuxR proteins, the AHL-LuxR complex activates the Lux promoter. The threshold [AHL] at which switching occurs is determined by the affinity of AHL for the particulr LuxR ortholog. about quorum sensing)

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