Team:UNIPV-Pavia/Notebook/Week3Sep

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<div>
<div>
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<html><a name="week_start"></a></html>
= Week from September 14th, to September 20th, 2009 =
= Week from September 14th, to September 20th, 2009 =
<html>
<html>
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<tr>
<tr>
<td align="left" width="50%">
<td align="left" width="50%">
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Sep">
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Sep#week_start">
  <img src="https://2009.igem.org/Special:FilePath?file=Left_arrow.png"/>
  <img src="https://2009.igem.org/Special:FilePath?file=Left_arrow.png"/>
</a>
</a>
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Sep">Previous Week</a>
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Sep#week_start">Previous Week</a>
</td>
</td>
<td align="right" width="50%">  
<td align="right" width="50%">  
-
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week4Sep">Next Week</a>
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week4Sep#week_start">Next Week</a>
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week4Sep">
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week4Sep#week_start">
  <img src="https://2009.igem.org/Special:FilePath?file=Right_arrow.png"/>
  <img src="https://2009.igem.org/Special:FilePath?file=Right_arrow.png"/>
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== <html><font class="dayw_style">September, 14th</font></html> ==
== <html><font class="dayw_style">September, 14th</font></html> ==
 +
*Cloning
 +
*Wiki updating
<div align="right">
<div align="right">
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== <html><font class="dayw_style">September, 15th</font></html> ==
== <html><font class="dayw_style">September, 15th</font></html> ==
 +
*Fermentation experiment setup
 +
*Wiki updating
<div align="right">
<div align="right">
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== <html><font class="dayw_style">September, 16th</font></html> ==
== <html><font class="dayw_style">September, 16th</font></html> ==
 +
*Fermentation experiment
 +
*Wiki updating
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== <html><font class="dayw_style">September, 17th</font></html> ==
== <html><font class="dayw_style">September, 17th</font></html> ==
 +
''Experiment with Tecan F200''
 +
*<html><a href="http://aimed11.unipv.it/iGEM2009/TestTECANInfiniteF200/A1A2A7 TEST 17-09-09.pdf" target="_blank">Download Protocol</a></html>
 +
*<html><a href="http://aimed11.unipv.it/iGEM2009/TestTECANInfiniteF200/Ethanol test TEST 17-09-09.pdf" target="_blank">Download Protocol</a></html>
===== pH sensor =====
===== pH sensor =====
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**RBS33
**RBS33
**A2
**A2
-
**pNhaA + GFP (from now on it we'll be indicated only as pNhaA)
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**K116002 (from now on it will be indicated only as pNhaA)
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*Overnight incubation of the three culctures.
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*Overnight incubation of these three cultures at 37°C, 220 rpm.
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===== pH sensor =====
===== pH sensor =====
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*We put 50 ul into 5 ml LBK + Amp falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four hours and a half.
+
''1st experiment''
-
*OD measure of the three falcon with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
+
*We put 50 ul into 5 ml LBK + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four hours and a half at 37°C, 220 rpm.
 +
*OD measure of the three falcons with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
*We diluted:
*We diluted:
**RBS into:
**RBS into:
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*** LBK + Amp pH 7,5
*** LBK + Amp pH 7,5
*** LBK + Amp pH 8,5
*** LBK + Amp pH 8,5
 +
*We started this 6 hours' experiment that performed absorbance and fluorescence measures (on three different wells) for each sample every 5 minutes.
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== <html><font class="dayw_style">September, 19th</font></html> ==
== <html><font class="dayw_style">September, 19th</font></html> ==
 +
*Wiki updating
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[[#top|Top]]
[[#top|Top]]
</div>
</div>
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== <html><font class="dayw_style">September, 20th</font></html> ==
 
 +
== <html><font class="dayw_style">September, 20th</font></html> ==
 +
*Cloning
 +
*Wiki updating
<div align="right">
<div align="right">
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<tr>
<tr>
<td align="left" width="50%">
<td align="left" width="50%">
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<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Sep">
+
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Sep#week_start">
  <img src="https://2009.igem.org/Special:FilePath?file=Left_arrow.png"/>
  <img src="https://2009.igem.org/Special:FilePath?file=Left_arrow.png"/>
</a>
</a>
-
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Sep">Previous Week</a>
+
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week2Sep#week_start">Previous Week</a>
</td>
</td>
<td align="right" width="50%">  
<td align="right" width="50%">  
-
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week4Sep">Next Week</a>
+
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week4Sep#week_start">Next Week</a>
-
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week4Sep">
+
<a href="https://2009.igem.org/Team:UNIPV-Pavia/Notebook/Week4Sep#week_start">
  <img src="https://2009.igem.org/Special:FilePath?file=Right_arrow.png"/>
  <img src="https://2009.igem.org/Special:FilePath?file=Right_arrow.png"/>
</a>
</a>

Latest revision as of 23:38, 21 October 2009

EthanolPVanimation.gif

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September 2009
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October 2009
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Week from September 14th, to September 20th, 2009

Previous Week Next Week

September, 14th

  • Cloning
  • Wiki updating

Top

September, 15th

  • Fermentation experiment setup
  • Wiki updating

Top

September, 16th

  • Fermentation experiment
  • Wiki updating


Top

September, 17th

Experiment with Tecan F200

pH sensor
  • Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
    • RBS33
    • A2
    • K116002 (from now on it will be indicated only as pNhaA)
  • Overnight incubation of these three cultures at 37°C, 220 rpm.


Top

September, 18th

pH sensor

1st experiment

  • We put 50 ul into 5 ml LBK + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four hours and a half at 37°C, 220 rpm.
  • OD measure of the three falcons with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
  • We diluted:
    • RBS into:
      • LBK + Amp pH 5,5
      • LBK + Amp pH 6,6
      • LBK + Amp pH 7,5
      • LBK + Amp pH 8,5
    • pNhaA into:
      • LBK + Amp pH 5,5
      • LBK + Amp pH 6,6
      • LBK + Amp pH 7,5
      • LBK + Amp pH 8,5
    • A2 into:
      • LBK + Amp pH 5,5
      • LBK + Amp pH 6,6
      • LBK + Amp pH 7,5
      • LBK + Amp pH 8,5
  • We started this 6 hours' experiment that performed absorbance and fluorescence measures (on three different wells) for each sample every 5 minutes.


Top

September, 19th

  • Wiki updating


Top

September, 20th

  • Cloning
  • Wiki updating

Top

Previous Week Next Week
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