Team:Tokyo-Nokogen/Project

From 2009.igem.org

(Difference between revisions)

Revision as of 00:02, 22 October 2009

In biochemistry experiments, expression and getting objective protein is popular. But the procedure is almost vexatious complication due to need many solutions and centrifugations. So we hope to solve this trouble to construct alternative easily system with synthetic biology. The system we named Escherichia coli auto protein synthesizer. It has three components. 1. light switches 2. Aggregation 3. Signal count system. A figure that shows procedures this system is below. We only have to irradiate E.coli three times and purification to get proteins in it.

By first induction with red light, objective protein is expressed in E.coli. Then same induction repeated, but Antigen 43 that is protein for aggregation E.coli is expressed by signal count system. Once medium replaced, we think that we use blue light with phycoerythrine as receptor. Thereby, holin and endolysin are expressed and lysis E.coli. Finally, by purification of objective protein, this system is completed.

@@ Line 53: / Line 53: @@
-<th scope="row"><a href="https://2009.igem.org/Team:Tokyo-Nokogen" onMouseOut="MM_swapImgRestore()" onMouseOver="MM_swapImage('Image6','','https://static.igem.org/mediawiki/2009/e/e6/Home2.png',1)"><img src="https://static.igem.org/mediawiki/2009/4/41/Home3.png" alt="" name="Image6" width="100" height="35" border="0" align="" style="margin-left:0px"></th>
+<th scope="row"><a href="https://2009.igem.org/Team:Tokyo-Nokogen" onMouseOut="MM_swapImgRestore()" onMouseOver="MM_swapImage('Image6','','https://static.igem.org/mediawiki/2009/e/e6/Home2.png',1)"><div align="center"><img src="https://static.igem.org/mediawiki/2009/4/41/Home3.png" alt="" name="Image6" width="100" height="35" border="0"></div></th>
-<td><a href="https://2009.igem.org/Team:Tokyo-Nokogen/Project" onMouseOut="MM_swapImgRestore()" onMouseOver="MM_swapImage('Image8','','https://static.igem.org/mediawiki/2009/f/f9/Project2.png',1)"><img src="https://static.igem.org/mediawiki/2009/9/9d/Project3.png" alt="" name="Image8" width="120" height="35" border="0" align="middle" style="margin-left:10px"></td>
+<td><a href="https://2009.igem.org/Team:Tokyo-Nokogen/Project" onMouseOut="MM_swapImgRestore()" onMouseOver="MM_swapImage('Image8','','https://static.igem.org/mediawiki/2009/f/f9/Project2.png',1)"><div align="center"><img src="https://static.igem.org/mediawiki/2009/9/9d/Project3.png" alt="" name="Image8" width="120" height="35" border="0"></div></td>
-<td><a href="https://2009.igem.org/Team:Tokyo-Nokogen/Members" onMouseOut="MM_swapImgRestore()" onMouseOver="MM_swapImage('Image10','','https://static.igem.org/mediawiki/2009/a/a6/Members2.png',1)"><img src="https://static.igem.org/mediawiki/2009/a/aa/Members3.png" alt="" name="Image10" width="120" height="35" border="0" align="middle" style="margin-left:10px">
+<td><a href="https://2009.igem.org/Team:Tokyo-Nokogen/Members" onMouseOut="MM_swapImgRestore()" onMouseOver="MM_swapImage('Image10','','https://static.igem.org/mediawiki/2009/a/a6/Members2.png',1)"><div align="center"><img src="https://static.igem.org/mediawiki/2009/a/aa/Members3.png" alt="" name="Image10" width="120" height="35" border="0"></div></td>
 <tr>
@@ Line 65: / Line 65: @@
 <th scope="row"><a href="https://2009.igem.org/Team:Tokyo-Nokogen/Parts"
-onMouseOut="MM_swapImgRestore()"onMouseOver="MM_swapImage('Image12','','https://static.igem.org/mediawiki/2009/e/e6/Parts2.png',1)"><img src="https://static.igem.org/mediawiki/2009/3/34/Parts3.png" alt="" name="Image12" width="100" height="35" border="0" align="middle" style="margin-left:0px"></th>
+onMouseOut="MM_swapImgRestore()"onMouseOver="MM_swapImage('Image12','','https://static.igem.org/mediawiki/2009/e/e6/Parts2.png',1)"><div align="center"><img src="https://static.igem.org/mediawiki/2009/3/34/Parts3.png" alt="" name="Image12" width="100" height="35" border="0"></div></th>
-<td><a href="https://2009.igem.org/Team:Tokyo-Nokogen/notebook" onMouseOut="MM_swapImgRestore()" onMouseOver="MM_swapImage('Image14','','https://static.igem.org/mediawiki/2009/b/bd/Notebook2.png',1)"><img src="https://static.igem.org/mediawiki/2009/0/0b/Notebook3.png" alt="" name="Image14" width="130" height="35" border="0" align="middle" style="margin-left:10px"></div></a></td>
+<td><a href="https://2009.igem.org/Team:Tokyo-Nokogen/notebook" onMouseOut="MM_swapImgRestore()"onMouseOver="MM_swapImage('Image14','','https://static.igem.org/mediawiki/2009/b/bd/Notebook2.png',1)"><div align="center"><img src="https://static.igem.org/mediawiki/2009/0/0b/Notebook3.png" alt="" name="Image14" width="130" height="35" border="0"></div></td>
-<td>&nbsp;</td>
+<td><a href="https://2009.igem.org/Team:Tokyo-Nokogen/notebook" onMouseOut="MM_swapImgRestore()"onMouseOver="MM_swapImage('Image14','','https://static.igem.org/mediawiki/2009/b/bd/Notebook2.png',1)"><div align="center"><img src="https://static.igem.org/mediawiki/2009/e/eb/Safety3.png" alt="" name="Image14" width="110" height="35" border="0"></div></td>
 			      </table>