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Making delayed-LuxR mutants
From 2009.igem.org
(Difference between revisions)
(New page: ===Experiments=== # luxR gene was mutated by error-prone PCR using Mn2+(Joyce, 1996) and cloned it into a pUC vector. # This library was transformed into XL10-Gold, and the plasmid was mi...) |
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# luxR gene was mutated by error-prone PCR using Mn2+(Joyce, 1996) and cloned it into a pUC vector. | # luxR gene was mutated by error-prone PCR using Mn2+(Joyce, 1996) and cloned it into a pUC vector. | ||
# This library was transformed into XL10-Gold, and the plasmid was mini-prepped to get an plasmid library (pUC-[luxR]). | # This library was transformed into XL10-Gold, and the plasmid was mini-prepped to get an plasmid library (pUC-[luxR]). | ||
| - | # The plasmid library was transformed into | + | # The plasmid library was transformed into JW1226 harboring pAC-plux-gfp, which will glow green when an active LuxR was present. |
# We screened for the fast/dull mutant of LuxR, and collected the clone's plasmid. | # We screened for the fast/dull mutant of LuxR, and collected the clone's plasmid. | ||
Latest revision as of 00:15, 22 October 2009
Experiments
- luxR gene was mutated by error-prone PCR using Mn2+(Joyce, 1996) and cloned it into a pUC vector.
- This library was transformed into XL10-Gold, and the plasmid was mini-prepped to get an plasmid library (pUC-[luxR]).
- The plasmid library was transformed into JW1226 harboring pAC-plux-gfp, which will glow green when an active LuxR was present.
- We screened for the fast/dull mutant of LuxR, and collected the clone's plasmid.
