Team:Chiba/Project/medium

From 2009.igem.org

(Difference between revisions)

Revision as of 00:28, 22 October 2009

First, we made 4 styles of medium.

No.	1	2	3	4
agar [%]	1	1	5	5
Thickness [mm]	1.5	7.0	1.5	7.0

We put Nitro Cellulose filter on these 4 styles of medium and cultured 13 of mutants LuxR each 3 colonies.

After cultivation, the mudium gel with nitro cellulose filter was quarried and layered on 2000 nM AHL containing media with same thickness. Thereafter We obtained the fluorescence pictures.

Results

1

2

3

4

The style 4 medium afforded the most delayed time among the LuxR mutants.

Namely, the thin medium of agar was better for our animation than the thick medium.

@@ Line 33: / Line 33: @@
-After culture, Nitro Cellulose filterごとそれぞれの培地をカッターで切り出して同じ厚さの2000 nMのAHLを含む培地にのせて目視で蛍光の観察を始めた。
+After cultivation, the mudium gel with nitro cellulose filter was quarried and layered on 2000 nM AHL containing media with same thickness. Thereafter We obtained the fluorescence pictures.
-*Results/結果
+*Results
 <gallery>
@@ Line 46: / Line 46: @@
+The style 4 medium afforded the most delayed time among the LuxR mutants.
+Namely, the thin medium of agar was better for our animation than the thick medium.
-この実験をした結果、各mutant LuxRが光りだす時刻の差は、4の培地で実験を行ったときに最も大きい差が見られた。
-つまり厚さが薄かったりagar が少なかったりした培地上でのmutant LuxRが光りだす時刻のばらつき具合よりも、この4の培地上でのばらつき具合の方がはっきりしていた。
-だから私たちはこの培地条件を使ってdemoをすることにした。