Team:Chiba/Project/medium

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(Difference between revisions)

Latest revision as of 00:38, 22 October 2009

First, we made 4 styles of medium.

No.	1	2	3	4
agar [%]	1	1	5	5
Thickness [mm]	1.5	7.0	1.5	7.0

We put Nitro Cellulose filter on these 4 styles of medium and cultured 13 of mutants LuxR each 3 colonies.

After cultivation, the mudium gel with nitro cellulose filter was quarried and layered on 2000 nM AHL containing media with same thickness. Thereafter We obtained the fluorescence pictures.

Results

1

2

3

4

The No.4 medium afforded the most delayed time among the LuxR mutants.

Namely, the thin medium of agar was better for our animation than the thick medium.

@@ Line 1: / Line 1: @@
-まず、次の４種類の培地を作成した。
+First, we made 4 styles of medium.
@@ Line 19: / Line 19: @@
 </tr>
 <tr>
-<td>培地の厚さ [mm]</td>
+<td>Thickness [mm]</td>
 <td></td>
 <td>1.5</td>
@@ Line 30: / Line 30: @@
-この４種類の培地上にNitro Cellulose filterをのせ、その上でそれぞれ13種のMutantsを3 coloniesずつ培養した。
+We put Nitro Cellulose filter on these 4 styles of medium and cultured 13 of mutants LuxR each 3 colonies.
-培養後、Nitro Cellulose filterごとそれぞれの培地をカッターで切り出して同じ厚さの2000 nMのAHLを含む培地にのせて目視で蛍光の観察を始めた。
+After cultivation, the mudium gel with nitro cellulose filter was quarried and layered on 2000 nM AHL containing media with same thickness. Thereafter We obtained the fluorescence pictures.
+*Results
+<gallery>
+Image:Chiba_10mL1%25mts-GFPuv1625.jpg|1
+Image:Chiba_40mL1%25mts-GFPuv1625.jpg|2
+Image:Chiba_10mL5%25mts-GFPuv1625.jpg|3
+Image:Chiba_40mL5%25mts-GFPuv1625.jpg|4
+</gallery>
+The No.4 medium afforded the most delayed time among the LuxR mutants.
+Namely, the thin medium of agar was better for our animation than the thick medium.
-この実験をした結果、各mutant LuxRが光りだす時刻の差は、4の培地で実験を行ったときに最も大きい差が見られた。
-つまり厚さが薄かったりagar が少なかったりした培地上でのmutant LuxRが光りだす時刻のばらつき具合よりも、この4の培地上でのばらつき具合の方がはっきりしていた。
-だから私たちはこの培地条件を使ってdemoをすることにした。