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| - | __NOTOC__
| + | {{Template:LCG_top_Netscape}}<!--Do not remove the first and last lines in this page!--> |
| - | <html> | + | |
| | | | |
| - | <head>
| + | {| |
| | + | |-valign="top" border="0" |
| | + | |width="900px" style="padding: 0 20px 0 0;"| |
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| - | <!--Script for looking for mimeTypes (Flash player!!!)-->
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| - | <script language="JavaScript">
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| - | var mime;
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| - | if(navigator.mimeTypes != 0){
| + | ='''The Project in a nutshell'''= |
| - | mime = "mimeTypes";
| + | |
| - | }
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| - | mime="noMimeTypes";
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| - | document.write("Sorry, no flash" + mime);
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| + | |
| - | <!--Script for changing images-->
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| - | function mouseOver(){
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| - | document.getElementById("b1").src ="https://static.igem.org/mediawiki/2009/6/64/Phages_flash_player_b.png";
| + | |
| - | }
| + | |
| - | function mouseOut(){
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| - | }
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| | + | Bacteriophage infection represents an interesting process in microbiology and industry. The idea of being able to contend at a population level with such infections is the main motivation for the development of our project.<br> |
| | | | |
| - | </script> | + | We propose a population level approach relaying on a defense system delivered by an engineered version of the enterobacteria phage P4. The purpose of the defense construction is to modify bacteria in order to hold back the process of infection by triggering a cellular death response when a cell encounters a specific component of the infective phage. Such response will be fast enough to stop the formation process of viral particles, thus preventing the phage proliferation and population decline.<br> |
| | | | |
| | + | The delivery system takes advantage of the satellite properties of P4 phage. This means that a P4 phage engineered with the defense construction will be able to infect an ''E.coli'' strain which harbors some genes from helper phage P2 that are used for complementing and completing P4 life cycle, hence creating a production line of our version of P4.<br> |
| | + | Besides, the defense system will consist of DNA and RNA degradation by toxins which will be transcribed by T3 or T7 RNA-Polymerases fast enough to stop phage assembly and scattering in the environment. Simultaneously, a quorum sensing signal will be diffusing to the non-infected bacteria acting as a transcriptional activator of an antisense RNA against bacteriophage's transcriptional machinery , hence "warning" the population to prepare against further T3 or T7 infection.<br> |
| | + | <br> |
| | + | We implemented a stochastic [[Team:LCG-UNAM-Mexico: | multi-scale model]]. The model will simulate the behaviour at the intracellular scale using [[Team:LCG-UNAM-Mexico:Molecular model | stochastic molecular simulations]] and at the populations scale using a [[Team:LCG-UNAM-Mexico:CA | Cellular Automata]] and a [[Team:LCG-UNAM-Mexico:odes | system of ODE's]]. <br> Simulations results are in good agreement with existing experimental data. Thanks to the structure and design of the model this can be easily modified in order to simulate infection dynamics for different bacteria and phages. Furthermore, our Molecular model can be used as a reliable tool for sampling biomolecules distributions involved in phage infection processes. <br> |
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| - | </head> | + | <html> |
| - | | + | <header> |
| - | <style> | + | <script language="JavaScript"> |
| - | body{
| + | function mouseOver_comic(){ |
| - | background:url(https://static.igem.org/mediawiki/2009/a/a8/BG4.png) repeat top left;
| + | document.getElementById("comic").src ="https://static.igem.org/mediawiki/2009/6/60/Comic_LCG_bb.png"; |
| | + | } |
| | + | function mouseOut_comic(){ |
| | + | document.getElementById("comic").src ="https://static.igem.org/mediawiki/2009/a/a8/Comic_LCG_ba.png"; |
| | } | | } |
| - | h1.firstHeading { display: none; }
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| - | p {text-align: justify;}
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| - | a:link { color: #659D32; text-decoration: none}
| + | </script> |
| - | a:visited { color#659D32; text-decoration: none}
| + | </header> |
| - | a:hover { color:#f29400; text-decoration: none}
| + | |
| - | a:active { color:#f29400; text-decoration: none}
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| - | | + | |
| - | #bodyContent { padding: 10px auto; width: 900px; margin: auto; clear: none; }
| + | |
| - | | + | |
| - | table#team_members { text-align: justify; border: 0; }
| + | |
| - | table#team_members h2, table#team_members h3 { clear: both; }
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| - | h1.firstHeading { display: none; }
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| - | | + | |
| - | p {text-align: justify;}
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| | + | <style> |
| | + | #bodyContent { padding: 10px auto; width: 1000px; margin: auto; clear: none; } |
| | a:link { color: #659D32; text-decoration: none} | | a:link { color: #659D32; text-decoration: none} |
| - | a:visited {color:#659D32; text-decoration: none} | + | a:visited { color:#659D32; text-decoration: none} |
| | a:hover { color:#f29400; text-decoration: none} | | a:hover { color:#f29400; text-decoration: none} |
| | a:active { color:#f29400; text-decoration: none} | | a:active { color:#f29400; text-decoration: none} |
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| - | #bodyContent { padding: 10px auto; width: 900px; margin: auto; clear: none; }
| + | </style> |
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| - |
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| - | /*
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| - | Script-Free 3-Level Menu 1.3.4
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| - | www.CesarDaniel.info/Tech/ScriptFree3LevelMenu.html
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| - | ...............................................................................................
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| - | Sections
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| - |
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| - | * Reset
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| - | * Menu Bar
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| - | * Drop-Down Menus
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| - | * Side Menus
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| - | * Browser Specific
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| - | * Default Colors (Required)
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| - |
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| - | */
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| - | /*---------------------------------------------------------------------------------------- Reset */
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| - | div.MenuBar,
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| - | div.MenuBar ul,
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| - | div.MenuBar li,
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| - | div.MenuBar a,
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| - | div.MenuBar table,
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| - | div.MenuBar span {
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| - | margin: 0;
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| - | border: 0;
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| - | padding: 0;
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| - | z-index: 255;
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| - | }
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| - | div.MenuBar ul li ul.DropDownMenu {
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| - | display: none; /* Hides all drop-down menus. */
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| - | }
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| - | /*
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| - | li:hover works in IE7 and FF2.
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| - | a:hover works in IE6 and FF2.
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| - | a:hover breaks li:hover in FF2.
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| - | */
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| - | div.MenuBar ul li:hover ul.DropDownMenu li ul.SideMenu,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a ul.SideMenu {
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| - | display: none; /* Hides all side menus. */
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| - | }
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| - | /*------------------------------------------------------------------------------------- Menu Bar */
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| - | div.MenuBar {
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| - | font: 13px Arial, Helvetica, sans-serif;
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| - | height: 25px; /* Total height 27px. */
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| - | width: 900px;
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| - | }
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| - | div.MenuBar ul li {
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| - | display:block;
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| - | float: left;
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| - | list-style-type: none !important;
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| - | margin: 0 !important;
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| - | position: relative; /* Sets the positioning context for each drop-down menu. */
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| - | }
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| - | div.MenuBar ul li a {
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| - | display: block;
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| - | height: 17px; /* Keep height + padding-top + padding-bottom sync with the menu bar height. */
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| - | padding-top: 4px;
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| - | padding-bottom: 4px;
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| - | padding-left: 5em; /* Sets the left space between top-level items. */
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| - | padding-right: 5em; /* Sets the right space between top-level items. */
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| - | text-decoration: none !important;
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| - | }
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| - | /*------------------------------------------------------------------------------ Drop-Down Menus */
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| - | div.MenuBar ul li:hover ul.DropDownMenu,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu {
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| - | display: block;
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| - | width: 13em; /* Drop-down menu width.
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| - | Use MenuTailor.css to customize. */
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| - | padding: 1px; /* Sets the drop-down menu "effective border" width. */
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| - | position: absolute;
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| - | top: 25px; /* Places the drop-down menu under the menu bar.
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| - | Keep it sync with the menu bar height. */
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| - | left: 0; /* Aligns the drop-down menu to its top-level item. */
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| - | }
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| - | div.MenuBar ul li:hover ul.DropDownMenu li a,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a {
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| - | width: 13em; /* Keep it sync with the drop-down menu width.
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| - | Use MenuTailor.css to customize. */
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| - | padding-left: 0;
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| - | padding-right: 0;
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| - | }
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| - | ul.DropDownMenu li a span {
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| - | display: block;
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| - | padding-left: 0.75em; /* Sets the left space of each drop-down menu item. */
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| - | padding-right: 0.25em; /* Sets the right space of each drop-down menu item. */
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| - | text-align: right; /* Aligns the >> symbol to the right. */
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| - | }
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| - | ul.DropDownMenu li a span span {
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| - | float: left; /* Aligns the text (back) to the left. */
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| - | }
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| - | /*----------------------------------------------------------------------------------- Side Menus */
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| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
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| - | display: block;
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| - | width: 11em; /* Side menu width.
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| - | Use MenuTailor.css to customize. */
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| - | padding: 1px; /* Sets the side menu "effective border" width. */
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| - | position: absolute;
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| - | top: -1px; /* Aligns the side menu to its drop-down menu item.
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| - | Keep it sync with the side menu "effective border" width. */
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| - | left: 13em; /* Places the side menu to the right of the drop-down menu.
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| - | Keep it sync with the drop-down menu width.
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| - | Use MenuTailor.css to customize. */
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| - | }
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| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
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| - | width: 11em; /* Keep it sync with the side menu width.
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| - | Use MenuTailor.css to customize. */
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| - | }
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| - | div.MenuBar ul li ul.DropDownMenu li ul.SideMenu li a span {
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| - | padding-left: 1.5em; /* Sets the left space of each side menu item. */
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| - | padding-right: 0.5em; /* Sets the right space of each side menu item. */
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| - | text-align: left;
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| - | }
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| - | /*----------------------------------------------------------------------------- Browser Specific */
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| - | * html div.MenuBar {
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| - | position: relative; /* For IE55 and IE6.
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| - | Hidden (* html) from non-IE browsers. */
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| - | }
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| - | * html div.MenuBar ul li a {
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| - | float: left; /* For IE55 and IE6.
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| - | Breaks O9.
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| - | Hidden (* html) from non-IE browsers. */
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| - | }
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| - | * html ul.DropDownMenu li a:hover {
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| - | cursor: hand; /* For IE55.
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| - | Hidden (* html) from non-IE browsers. */
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| - | }
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| - | ul.DropDownMenu li a:hover {
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| - | cursor: pointer; /* For IE6 and IE7.
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| - | Hidding it (* html) from non-IE browsers breaks IE7! */
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| - | }
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| - | * html div.MenuBar a:hover {
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| - | text-decoration: none; /* For IE55 and IE6.
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| - | Hidden (* html) from non-IE browsers. */
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| - | }
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| - | * html div.MenuBar ul li table,
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| - | * html div.MenuBar ul li table td {
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| - | border: 0; /* For IE55 and IE6.
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| - | Hidden (* html) from non-IE browsers. */
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| - | }
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| - | /*------------------------------------------------------------------------------- Default Colors */
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| - | div.MenuBar {
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| - | background-color: #87C988;
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| - | border-color: #6EA06E;
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| - | }
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| - | div.MenuBar a {
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| - | background-color: #87C988; /* Top-level unselected items. */
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| - | color: white;
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| - | }
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| - | div.MenuBar ul li:hover a,
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| - | div.MenuBar ul li a:hover {
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| - | background-color: #3D602F; /* Top-level selected item. */
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| - | color: white;
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| - | }
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| - | /*...............................................................................................*/
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| - | div.MenuBar ul li:hover ul.DropDownMenu,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu {
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| - | background-color: #87C988; /* Sets the drop-down menu "effective border" color. */
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| - | }
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| - | div.MenuBar ul li:hover ul.DropDownMenu li a,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a {
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| - | background-color: #87C988; /* Drop-down menu unselected items.
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| - | Sets the drop-down menu "effective background" color. */
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| - | color: white;
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| - | }
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| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover {
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| - | background-color: #3D602F; /* Drop-down menu selected item. */
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| - | color: white;
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| - | }
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| - | /*...............................................................................................*/
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| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
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| - | background-color: #87C988; /* Sets the side menu "effective border" color. */
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| - | }
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| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
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| - | background-color: #87C988; /* Side menu unselected items.
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| - | Sets the side menu "effective background" color. */
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| - | color: white;
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| - | }
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| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
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| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
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| - | background-color: #3D602F; /* Side menu selected item. */
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| - | color: white;
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| - | }
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| - | /*-----------------------------------------------------------------------------------------------*/
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| - | </style>
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| | <body> | | <body> |
| | | | |
| | + | <a href="https://2009.igem.org/Team:LCG-UNAM-Mexico:Comic" ><img border="0" src="https://static.igem.org/mediawiki/2009/a/a8/Comic_LCG_ba.png" id="comic" onmouseover="mouseOver_comic()" onmouseout="mouseOut_comic()"></a> |
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| - | <!-- Begin of Checking for Flash availability -->
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| - | <script language="JavaScript">
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| - | <!--document.write("funciono");-->
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| - | if(mime=="mimeTypes"){
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| - | if(navigator.mimeTypes["application/x-shockwave-flash"]){
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| - | <!--document.write("Yey!!! tengo flash mira!!!" + mime);-->
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| - | document.write('<div id="header"><EMBED src="https://static.igem.org/mediawiki/2009/b/bc/Banner_LCG.swf" quality=high bgcolor=#FFFFFF WIDTH="900" HEIGHT="207"></EMBED></div>');
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| - | }
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| - | }
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| - | else{
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| - | mime="noMimeTypes";
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| - | <!--document.write("Sorry, no flash" + mime);-->
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| - | document.write('<table border="0" width="900" cellpadding="80"><tr><th><a href="http://get.adobe.com/es/flashplayer/"><img border="0" height="172" width="200" src="https://static.igem.org/mediawiki/2009/b/b2/Phages_flash_player_a.png" id="b1" onmouseover="mouseOver()" onmouseout="mouseOut()"></a></th><th><p><a href"Flashless.html"><img border="0" height="172" width="200" src="https://static.igem.org/mediawiki/2009/d/d0/Nonflash_a.png" id="b2" onmouseover="mouseOver2()" onmouseout="mouseOut2()"></a></th></tr></table>');
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| - | </script>
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| - | <!-- Begin of Script-Free 3-Level Menu 1.3 Example -->
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| - | <div class="MenuBar" id="LCG">
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| - | <ul>
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| - | <li>
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| - | <a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/intento1" onMouseOver="window.status= 'Click here to return Home'; return true;">Home
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| - | <!--[if gt IE 6]><!--></a><!--<![endif]-->
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| - | <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
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| - | <!--[if lte IE 6]></td></tr></table></a><![endif]-->
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| - | </li>
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| - | <a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Record">Record
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| - | <!--[if gt IE 6]><!--></a><!--<![endif]-->
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| - | <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
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| - | <a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Project">Project</a>
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| - | <ul class="DropDownMenu" id="Ddm1">
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| - | <li><a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Parts"><span><span>Parts</span></span></a></li>
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| - | <li><a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Resources"><span><span>Resources</span></span></a></li>
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| - | <li><a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Description"><span><span>Description</span>>></span></a>
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| - | <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
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| - | <ul class="SideMenu" id="Sm11">
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| - | <li><a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Description"><span><span>Design</span></span></a></li>
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| - | <li><a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Description"><span><span>Delivery</span></span></a></li>
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| - | <li><a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Description"><span><span>Defense</span></span></a></li>
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| - | <a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Team">Team</a>
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| - | <ul class="DropDownMenu" id="Ddm2">
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| - | <li><a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Team"><span><span>Aknowledgments</span></span></a></li>
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| - | <li><a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Team"><span><span>Collaborations</span></span></a></li>
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| - | <li><a href="https://2009.igem.org/Team:LCG-UNAM-Mexico/Team"><span><span>Sponsors</span></span></a></li>
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| - | </ul>
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| - | <!-- End of Script-Free 3-Level Menu 1.3 Example -->
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| | </body> | | </body> |
| | </html> | | </html> |
| | | | |
| - | {|
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| - | |-valign="top" border="0"
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| - | |width="900px" style="padding: 0 20px 0 0;"|
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| - |
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| - |
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| - | =='''The Project'''==
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| - |
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| - |
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| - | Bacteriophage infection represents a common matter in science and industry. We propose to contend with such infections at a population level by triggering a defense system delivered by an engineered version of the satellite phage P4. This phage will be produced into an E. coli strain which harbors some genes from the helper phage P2 that are used for complementing and completing P4 life cycle, hence creating a production line of our version of P4.
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| - |
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| - | This will enable phage production and subsequent delivery of the defense system into wild-type E. coli and possibly other bacterial host strains.
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| - |
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| - | The defense will consist of DNA and RNA degradation by toxins which will be activated
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| - | by T3 or T7 RNA-Polymerases and transcribed fast enough to stop phage assembly and scattering in the environment.
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| - |
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| - | Moreover, the system includes the spread of a quorum sensing signal, hence "warning" the population to prepare against further T3 or T7 infection.
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| | | | |
| - | We will implement a stochastic population model to simulate the infection processes and quantify the efficiency of our system.
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"